臺灣博碩士論文加值系統

穗花蛇菰( Balanophora laxiflora Hemsl. )為台灣固有的一年生至多年生之全寄生性植物，其野生數量因人類採集而漸減，亟待建立人工栽培系統以滿足藥用需求。然而台灣穗花蛇菰種子發芽過程尚無文獻記載，冷清草為穗花蛇菰寄主之一，其植株小而數量多，本試驗探討建立冷清草繁殖系統，嘗試將穗花蛇菰種子寄生於冷清草，試驗結果如下：
一、冷清草
（一）試管外
冷清草種子發芽為需光性且種子存在於粒徑介於0.25至1.5 mm之森林土壤中。10 mg/l gibberellic acid ( GA )處理可提高發芽率至90%，添加森林土壤可使幼苗維持較佳生長情形，以扦插方式可快速獲得大量冷清草植株。
（二）試管內
紙橋法可使冷清草根部充分獲得伸展，具有較佳生長勢。然冷清草幼苗生長緩慢，播種120天後雙葉展開僅1.5 cm。單節扦插以0.1 mg/l 6-benzylaminopurine ( BA )處理有較高的發根率及株高，有利進行穗花蛇菰種子接種。
二、穗花蛇菰
（一）試管外
不同粒徑森林土壤添加發芽刺激物Nijmegen 1與GA可觀察到絲狀物形成，但非穗花蛇菰種子發芽。將粒徑小於0.25 mm森林細土接種於冷清草根部，可發現瘤狀物形成，但塑膠切片顯示瘤狀物並非蛇菰寄生。穗花蛇菰種子以不同濃度GA進行預培養，再以發芽刺激物Nijmegen 1誘導發芽，種子於形態上無改變，顏色呈褐色至深褐色，甚至呈現黑色。
（二）試管內
穗花蛇菰種子無菌播種一年後仍未發芽，但塑膠切片觀察確認所用材料是蛇菰種子，此為照片呈現之首例，有別以往模式圖，惟其活力與成熟度有待進一步驗證。將穗花蛇菰種子接種於冷清草根部並以玻片將種子與冷清草培養基隔開，處理後第二週部分種子褐化，形態上無改變，未見蛇菰種子發芽。由於玻片上根系無法在4週內恢復生長，試驗結果有待繼續觀察。

中文摘要…………………………………………………………….…..…….i
英文摘要……………………………………………………...….….…...…...iii
目次……………………………………………………………………...……v
表目次……………………………………………………….....…….…...…..vi
圖目次……………………………………………………..…………........…vii
壹、 前言…………………………………………………………...………..1
貳、 前人研究………………………………………………..….…..………3
參、 材料方法…………………………………………..………….………12
肆、 結果與討論……………………………………………...……………17
一、種子活力檢定—TTC法………………………………..……..……….17
二、冷清草種子發芽試驗…………………………………….….………...21
三、冷清草幼苗生長…………………………………………..……….….23
四、冷清草扦插繁殖………………………………………….…..……….26
五、穗花蛇菰種子發芽試驗….…………………………………….……...28
六、直播冷清草根部寄生物觀察…………………………………..……...33
七、森林細土接種後冷清草根部寄生物觀察…………………..…….…...36
八、試管內接種穗花蛇菰種子…………………………………..………...38
伍、 參考文獻……………………………………………...………………40

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