臺灣博碩士論文加值系統

目的：本研究在探討服用美國人蔘與游泳運動對果糖誘發胰島素阻抗（Insulin Resistant）大鼠葡萄糖代謝之影響。方法：本實驗針對28隻8週齡Wistar品系的雄性大鼠進行研究，先進行8週的果糖誘發胰島素阻抗，再將大鼠分為4組，分為控制組（Control）7隻、控制運動組（Exercise）7隻、人蔘組（Ginseng）7隻、人蔘運動組（Ginseng Exercise）7隻。分組後進行28天的游泳和美國人蔘攝食介入。28天介入後進行OGTT測試，觀察血糖濃度（GAUC、HOMA-IR），OGTT測試後1星期進行解剖，採取肝臟（Liver）、腓腸肌紅肌（RG）、腓腸肌白肌（WG）組織，並測試組織中肝醣含量與GLUT4的含量。結果：研究顯示，經過8週21%誘發胰島素阻抗的大鼠在經過28天游泳和美國人蔘攝食介入後，發現在血糖濃度GAUC數值，控制組和人蔘＋運動組相比，兩者之間有大的差異性（d＝1.476）；在血糖濃度HOMA-IR數值，控制組和人蔘＋運動組相比，兩者之間也有大的差異性（d＝1.544）。在肝臟、腓腸肌紅肌、白肌肝醣含量方面，控制組和人蔘＋運動組相比差異度都小（d＝0.122）。腓腸肌紅肌、白肌GLUT4含量，控制組和人蔘＋運動組相比差異度也都小（d＝0.312）。結論：得知服用100mg/kg美國人蔘加上每日游泳45分鐘的介入對胰島素阻抗的大鼠是可以更有效降低血糖濃度，但肝醣合成能力與肌肉中GLUT4的含量並非其達成其效果的主要原因。

關鍵字：HOMA-IR、GAUC、肝醣、GLUT4

Purpose: This study was to investigate the effects of American ginsengs combined with swimming on high-fructose diet induced insulin resistance rats. Methods: 28 eight weeks age Wister rats had free access to drinking water containing 21% fructose for 8 weeks to induce insulin resistance. The rats were divided into four groups randomly (Control, control exercise, ginseng, ginseng exercise), and underwent different treatments for another 28 days. After 28 days, OGTT was used to detect blood glucose (GAUC and HOMA-IR).One week later, the rats were anesthetized at which time liver was excised to measure glycogen, and red muscle of gastrocnemius and white muscle of gastrocnemius were excised to test GLUT4. Results: There were significant differences in the GAUC (d=1.476) and HOMA-IR (d=1.544) between control and ginseng exercise after 28 days intervention. Glycogen showed little difference in liver between control and ginseng exercise (d=0.122). Neither RG nor WG showed a difference ofGLUT4 between control and ginseng exercise (d=0.312). Conclusion: American ginsengs combined with swimming decreases blood glucose in insulin resistance rats, not due to the effects of glycogen and GLUT4.

Key word: HOMA-IR、GAUC、Glycogen、GLUT4

目 錄
第壹章　緒論 1
第一節 問題背景 1
第二節 研究目的 3
第三節 研究假設 4
第四節 名詞操作性定義 4
第貳章　文獻探討 7
第一節　胰島素阻抗(INSULIN RESISTANT)的定義 7
第二節　果糖(FRUCTOSE)誘發胰島素阻抗機轉 10
第三節　運動可以影響身體對胰島素的敏感度 12
第四節　人蔘對胰島素敏感度的影響 14
第五節　小結 16
第參章　研究方法與步驟 17
第一節　實驗對象 17
第二節　實驗設計與步驟 17
第三節 實驗介入與工具 19
第四節　血液與組織樣本處理分析 27
第五節　統計分析 29
第肆章　結果 31
第一節　果糖誘發後血糖值(GLUCOSE) 31
第二節、美國人蔘與游泳介入後其血糖濃度值 33
第三節 美國人蔘與游泳介入後肌肉與肝臟肝醣的含量 36
第四節 美國人蔘與游泳介入後肌肉中GLUT4的含量 40
第伍章　討論 43
第一節　誘發胰島素阻抗後其血糖變化 43
第二節　美國人蔘與游泳介入後其血糖濃度的變化 44
第三節　美國人蔘與游泳介入後肌肉與肝臟肝醣含量的變化 46
第四節　美國人蔘與游泳介入後肌肉中GLUT4含量的變化 47
第陸章　結論與建議 49
第一節　結論 49
第二節　建議 49
參考文獻 51
附 錄 67
附錄一 MERCODIA ULTRASENSITIVE RAT INSULIN ELISA 67
附錄二　肌肉肝醣分析(SKELETAL MUSCLE GLYCOGEN CONTENT ASSAY) 69
附錄三　LOWRY ASSAY PROTOCOL 73
附錄四　GLUT4西方點墨分析（WESTERN BLOTTING PROTEIN ASSAY） 75
附錄五　OGTT果糖組血糖值原始資料 83
附錄六 OGTT控制組血糖值原始資料 85
附錄七　血糖濃度GAUC、HOMA-IR原始資料 87
附錄八　肝醣含量原始資料 89
附錄九　GLUT4含量原始資料 91
附錄十 結果分析摘要表 93

表目錄
表1 GAUC變異數同質性檢定 93
表2 GAUC均等平均數的WELCH檢定 93
表3 GAUC單因子變異數分析摘要表 93
表4 HOMA-IR變異數同質性檢定 93
表5 HOMA-IR均等平均數的WELCH檢定 93
表6 HOMA-IR單因子變異數分析摘要表 94
表7 肝臟肝醣含量變異數同質性檢定 94
表8 肝臟肝醣含量單因子變異數分析摘要表 94
表9 腓腸肌紅肌肝醣含量變異數同質性檢定 94
表10 腓腸肌紅肌肝醣含量單因子變異數分析摘要表 94
表11 腓腸肌白肌肝醣含量變異數同質性檢定 95
表12 腓腸肌白肌肝醣含量單因子變異數分析摘要表 95
表13 腓腸肌紅肌GLUT4含量變異數同質性檢定 95
表14 腓腸肌紅肌GLUT4含量單因子變異數分析摘要表 95
表15 腓腸肌白肌GLUT4含量變異數同質性檢定 95
表16 腓腸肌白肌GLUT4含量均等平均數的WELCH檢定 96
表17 腓腸肌白肌GLUT4含量單因子變異數分析摘要表 96

 
圖目錄
圖1 胰島素對血漿中對葡萄糖的影響 8
圖2 升糖激素對血中葡萄糖的影響 9
圖3果糖誘發胰島素阻抗的機轉 11
圖4血糖機與血糖試紙 19
圖5 二次純水機 20
圖6 高速離心機 20
圖7迴轉式恆溫振盪器 21
圖8 微盤清洗儀 21
圖9多功能免疫分析儀 22
圖10先進標準型電子天平 22
圖11精密恆溫水槽 23
圖12分光光譜儀 23
圖13 組織均質機 24
圖14 電泳槽 24
圖15 電源供應器 25
圖16半濕式轉漬器 25
圖17冷光分析儀 26
圖18 OGTT測驗流程圖 27
圖19 OGTT 血糖趨勢圖 32
圖20 血糖濃度GAUC 34
圖21 血糖濃度HOMA-IR數值 35
圖22 肝臟肝糖含量 37
圖23 腓腸肌紅肌肝醣 38
圖24 腓腸肌白肌肝醣 39
圖25 腓腸肌紅肌GLUT4含量 41
圖26 腓腸肌白肌GLUT4含量 42

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