臺灣博碩士論文加值系統

在細胞生物學的研究上，蛋白質控制調節大部份細胞的生物活性，大多數的蛋白質會與其他蛋白質結合以進行細胞內的功能。在蛋白質交互作用的研究上，動力學分析的是相當有效的方法。光纖感知器具有系統架構簡單，塑膠光纖可用於一次性量測（disposable）的優點，可作為蛋白質動力學常數的量測工具。
有研究指出，C-反應蛋白（C-reactive protein, CRP），是一種發炎反應時所產生的急性相蛋白質，它亦被發現與心血管疾病有相關性。最近有研究則進一步指出另一種不同型態的CRP─mCRP和心血管疾病有更直接的相關性，本論文利用光纖生感知器測得mCRP與anti-CRP交互作用的動力學常數並提供對mCRP與心血管疾病的早期診斷研究之參數。

In cell biological research, protein control mediates many of biological activities of cells. Most of proteins function in association with other protein to gain an cellular function. Therefore protein-protein interaction becomes essential to understand biological activities that have been studied by determination of kinetic constants. The fiber optic biosensor is one of the easiest way to setup to study the kinetics of protein-protein interaction in real time where the optical fiber is disposable in the measurement. Then fiber optic biosensor is a novel equipment for kinetic constants measurement.
C-reactive protein (CRP), an acute phase protein of inflammation is related to the risk factor for cardiovascular diseases. In recently study, a different expression of CRP, the modified CRP (mCRP), shows more dependence on cardiovascular diseases significantly than CRP. In this study, a fiber optic biosensor was setup in order to determine mCRP/anti-CRP binding kinetics to investigate the condition between mCRP and the risk factor of cardiovascular diseases quantitatively. The experimental result will be useful for the purpose of earlier diagnosis of the cardiovascular diseases.

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