臺灣博碩士論文加值系統

本研究將Candida rugosa脂解酵素，以物理吸附方式固定於具有微孔的聚
丙烯擔體上，對此固定化酵素的溶液、溫度及貯存穩定性加以探討。應用
此固定化酵素及Lipozyme IM20脂解酵素催化Borage oil及Evening
primrose oil催化選擇性水解及酯化反應以濃縮γ-亞麻酯酸(GLA)。將溫
度、pH值控制於30℃、7.0下，以最佳酵素載量為75U Candida rugosa
Lipase/100mg PP對Borage oil水解反應的Michaelis常數及最大速率常數
分別是0.107M及393.89 U/mg protein，產物抑制反應之產物-酵素複合物
的解離常數為25mM。

Lipase(triacylglycerol ester hydrolase, E.C.3.1.1.3) from
Candida rugosa was immobilized on hydrophobic microporous
polypropylene supports by physical adsorption. The immobilized
enzyme catalysts were employed for the selective hydrolysis and
esterification of borage oil and evening primrose oil to
contrate their GLA contents in glycerides. The Michaelis
constant Km and the maximum rate constant Vm for the lipolysis
of borage oil by Candida rugosa are 0.107 M and 393.89 U/mg
protein, respectively. Product inhibition with a dissociation
constant of the enzyme-product complex Ki=25 mM was confirmed.