臺灣博碩士論文加值系統

將麗格秋海棠 ( Begonia × hiemalis‘Barkos’ ) 葉片及葉柄接種於含有NAA ( 0.1、1 mg/l ) 及BA ( 0.1、0.5、1、5 mg/l ) 不同濃度組合之MS培養基，培養八週後，以1 mg/l NAA及0.5 mg/l BA組合之MS培養基最佳，每葉片可誘導8.5個不定芽，每葉柄可誘導12.6個不定芽。切離位置以靠近葉柄部位的葉片，不定芽誘導率最高達100 %，每葉片可誘導8.4個不定芽，顯著地高於葉片中間部位及葉緣部位的培殖體。經由石蠟切片結果得知，麗格秋海棠葉片及葉柄誘導不定芽形成，是由表皮附近的薄壁細胞進行細胞分裂，而後分化形成具有莖頂分生組織和葉始原體等構造，並無完整體胚的形成，表示麗格秋海棠主要是由器官分化的途徑再生成植株。將葉片或葉柄誘導形成之不定芽，連同培殖體培養於各種MS鹽類濃度之固體或液體培養基進行不定芽生長，全量MS基本鹽類最適宜不定芽生長，將葉片誘導不定芽置於全量MS固體或液體培養基，每培殖體平均可再生成植株分別為10.9及12.2個植株，植株高度分布於2-4 cm。而麗格秋海棠葉柄誘導形成的不定芽，不論固體或液體培養基，僅少數芽發育至完整植株，僅2.1-4.6個植株。將瓶苗發根植株種植於栽培介質的盆中，內盛泥炭土、珍珠石、蛭石依等體積比均勻混合，四週後其存活率可達九成以上，經種植至開花外表型態觀察與母株無異。

Adventitious shoots were successfully regenerated using leaf or petiole of explants from Rieger Begonia ( Begonia × hiemalis ‘Barkos’ ). Leaf or petiole sections were cultured on MS medium containing different concentration of NAA ( 0.1、1 mg/l ) and BA ( 0.1、0.5、1、5 mg/l ). The culture medium was optimized for the leaf or petiole explants derived from shoot which was supplemented on MS medium with 1 mg/l NAA and 0.5 mg/l BA and observed 8.5 or 12.6 shoots from each explant after eight weeks, respectively. Hundred percent shoot regeneration and 8.4 shoots per explant was obtained when leaf cutting position near the petiole position of leaf explants and it was significantly different from leaf cutting position near the middle and margin of explants. Histological studies showed that adventitious shoots originated from both leaf or petiole epidermis layer and then differentiate to the formation of apical meristems and leaf primordial structures and it did not show intact somatic embryogenesis. Histological observation revealed that regeneration of leaf or petiole explants via organogenesis. Further elongation of adventitious shoots was obtained from each explant on full strength MS solid medium or liquid- shake medium with average of 10.9 and 12.2 plantlets, respectively. However, shoot only a few plantlets was obtained shoot of petiole explants on full strength MS solid medium or liquid-shake medium. All the regenerated plantlets were 90 % survival rate and continued to grow after the transfer to plastic pots. No morphological aberrations were observed in the regenerated plants.

誌謝.......................................................I
中文摘要..................................................II
英文摘要.................................................III
目錄......................................................IV
表目次...................................................VII
圖目次..................................................VIII
附錄目次..................................................IX
壹、前言...................................................1
貳、前人研究...............................................3
一、麗格秋海棠之遺傳組成與品種特性.....................3
二、影響麗格秋海棠組織培養之因子.......................4
(一) 光............................................4
(二) 溫度..........................................6
(三) 母株生育環境..................................6
(四) 發育時期......................................7
(五) 品種特性......................................8
(六) 接種方向......................................9
(七) 培養基組成...................................10
1. 生長調節劑................................10
2. 糖類......................................12
3. 氮源......................................13
4. 鹽基濃度..................................14
(八) 培養方式.....................................15
三、溫度及光週期對麗格秋海棠開花特性之影響............16
参、材料與方法............................................19
一、供試材料..........................................19
二、試驗方法..........................................19
(一) 不定芽的誘導.................................19
1. 試驗材料的消毒............................19
2. 葉片或葉柄不同培殖體切離位置對麗格秋海秋
棠不定誘導之影響..........................19
(二) 麗格秋海棠不定芽分化途徑之石蠟切片觀察.......20
(三) 影響不定芽生長的因子.........................20
1. MS鹽基濃度及固體或液體培養方式對麗格秋海
棠不定芽生長之影響........................20
2. 分切對麗格秋海棠葉片或葉柄不定芽生長之影響21
(四) 培養基成份及培養環境.........................21
1. 培養基成份................................21
2. 培養環境..................................21
(五) 再生植株生長開花特性之調查...................22
(六) 統計分析.....................................22
1. 試驗數據測定及計算標準....................22
2. 統計分析..................................23
肆、結果..................................................24
一、影響不定芽形成的因子..............................24
(一) 葉片或葉柄不同培殖體切離位置對麗格秋海棠不定芽
誘導之影響...................................24
(二) 不同麗格秋海棠品種對於誘導不定芽形成之影.....28
二、麗格秋海棠不定芽分化途徑之石蠟切片觀察............28
三、影響不定芽生長的因子..............................33
(一) MS鹽基濃度及固體或液體培養對麗格秋海棠不定芽生
長之影響.....................................33
(二) 分切對麗格秋海棠葉片或葉柄不定芽生長之影響...33
四、再生植株開花生長特性之調查........................44
(一) 再生植株健化.................................44
(二) 摘心對麗格秋海棠再生植株開花生長之影響.......44
伍、討論..................................................48
陸、參考文獻..............................................54
柒、附錄..................................................62

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