臺灣博碩士論文加值系統

本文章主要為從廢水處理之活性污泥萃取出的微生物溶解性產物（Soluble Microbial Products, SMP），鬆散鍵結性胞外聚合物質（Loosely Bound Extracellular Polymeric Substances, LBEPS）與緊密性鍵結胞外聚合物質（Tightly Bound Extracellular Polymeric Substances, TBEPS）中的溶解態有機物質（Dissolved Organic Matter, DOM），用尺寸排斥層析法（Size Exclusion Chromatography, SEC）依照分子量分類，利用紫外-可見光吸收光譜（波長230、254、280nm）、溶解性總有機碳（Total Dissolved Organic Carbon, DOC）與三維螢光（Excitation-Emission Matrix, EEM）作不同區間的特性分析。發現在SMP中分子量大於20000Da的有機物佔總體DOM的33%，芳香族類的蛋白質佔了其中大部分。LBEPS與TBEPS中DOM的組成大於20000Da的大分子約有22%，其組成份以腐質酸類的物質與蛋白質為主。多元氯化鋁（Polyaluminum Chloridel，PACl）可有效移除具大分子量之DOM。從EEM與UV吸收光譜可發現大分子量之蛋白質與腐質酸類在經過PACl加藥程序後被移除，但PACl無法移除分子量小於1000Da的DOM之低芳香性之有機酸類。由上述之實驗發現SMP與LBEPS之溶解態有機物的物理化學性質相似但不完全一樣，由SEC分離後之樣品經分析後發現TBEPS之組成與SMP及LBEPS相當不同。

並利用薄膜程序分析經過加藥條理後之水樣比較DOC之移除率與UV吸收光譜。發現經過UF膜（PES，0.22μm）DOC移除率可以達到80％以上，其中對薄膜阻塞的貢獻度以LBEPS最大。

The dissolved organic matters (DOM) in the soluble microbial products (SMP), loosely bound extracellular polymeric substances (LBEPS), and tightly bound extracellular polymeric substances (TBEPS) extracted from a wastewater sludge were fractionated using size exclusion chromatography, and were characterized by ultraviolet absorbance at 230, 254, and 280 nm, the dissolved organic carbon (DOC) contents, and the excitation-emission-matrix (EEM) spectra. The DOM of molecular weight (MW) > 20000 Da accounted for 33% for SMP, which incorporated proteins of high aromaticity, and 22% for both LBEPS and TBEPS, which were composed of organic acids-like substances and proteins respectively. Polyaluminum chloride (PACl) coagulation could effectively remove this fraction of DOM. On the other hand, the DOM of MW< 1000 Da were mostly non-aromatic organic acids (and probably also polysaccharides) of low aromaticity for SMP and both EPS, and were hardly removed by PACl coagulation. Based on the EEM and UV absorbance data, proteins of high MW and humic substances were easily removed by PACl coagulation. The present study indicates that, although the chemical characteristics of DOM in SMP and LBEPS were alike, but not identical. The DOM in TBEPS differed significantly from SMP or LBEPS based either on DOC distributions or on EEM fingerprints.

中文摘要………………………………………………………………………..Ⅰ
英文摘要………………………………………………………………………..Ⅱ
圖目錄………………………………………………………………………….. Ⅳ
表目錄...……………………………………………………………………….. Ⅵ
第一章 前言………………………………………………………………... 1
第二章 文獻回顧……………………………………………………………2
第三章 實驗步驟與藥品…………………………………………………....7
3-1 實驗藥品………………………………………………………………...7
3-2 實驗步驟………………...........................................................................9
第四章 結果與討論……………………………………………………… 12
4-1 萃取物之特性………………………………………………………... 15
4-2 SEC fraction …………………………………………………………...19
4-3 PACl混凝 ……………………………………………………………..30
4-4 加藥後過濾…………………………………………………………… .40
第五章 結論……………………………………………………………….. 50
參考文獻………………………………………………………………………..52

陳明源 “多重染色方案-胞外聚合物於生物聚集體之分佈”,碩士論文,國立台灣大學化學工程研究所,台北,2006

翁韻雅 “以高分子凝集劑處理高濁度原水之研究”,碩士論文,國立成功大學環境工程研究所,台南,2003

Amirthrajah,A.;O’Melia,C.R.,.”Coagulation and Floccuation,” Chap6,Water Quality and Treatment 5th,1990,AWWA, p.6.1

Aqino, S.F.D.,. ”Formation of soluble microbial products (SMP) in anaerobic digesters during stress conditions,” Ph.D. Thesis, 2004 Imperial College,London.

Boero, V. J., Eckenfelder, W. W., and Bowers, A. R., “Soluble Microbial Product Formation in Biological-Systems,” Water Science and Technology, 1991, 23(4-6), 1067-1076.

Bruus, J. H., Nielsen, P. H., and Keiding, K., “On the Stability of Activated-Sludge Flocs with Implications to Dewatering,” Water Research, 1992, 26 (12), 1597-1604.

Boero, V. J., Bowers, A. R., and Eckenfelder, W. W., “Molecular weight distribution of soluble microbial products in biological systems,” Water Science and Technology,
1996, 34(5-6), 241-248.

Bose, P.; Reckhow, D. A.,”Adsorption of natural organic matter on preformed aluminum hydroxide flocs,” Journal of Environmental Engineering-Asce,1998, 124 (9), 803-811.

Chudoba, J.,”Inhibitory Effect of Refractory Organic-Compounds Produced by Activated-Sludge Microorganisms on Microbial Activity and Flocculation,” Water Research, 1985, 19(2), 197-200.

Chaffin, W. L., Collins, B. , Marx, J. N., Cole, G. T. and Morrow, K. J., “Characterization of Mutant Strains of Candida-Albicans Deficient in Expression of a Surface Determinant,” Infection and Immunity ,1993, 61(8), 3449-3458.


Chu, H. P., Li, X. Y., “Membrane fouling in a membrane bioreactor (MBR): Sludge cake formation and fouling characteristics,” Biotechnology and Bioengineering 2005, 90(3), 323-331.

Cheryan, M. , and Rajagopalan, N., “Membrane processing of oily streams. Wastewater treatment and waste reduction,” Journal of Membrane Science , 1998, 151
(1), 13-28.

Chen, W., Westerhoff, P., Leenheer, J. A., Booksh, K.,”Fluorescence excitation - Emission matrix regional integration to quantify spectra for dissolved organic matter,” Environmental Science & Technology, 2003, 37(24), 5701-5710.

Comte, S., Gulbaud, G.., and Baudu, M., “Biosorption properties of extracellular polymeric substances (EPS) resulting from activated sludge according to their type: Soluble or bound,” Process Biochemistry, 2006, 41(4), 815-823.

Durmaz, B., and Sanin, F. D., “Effect of carbon to nitrogen ratio on the composition of microbial extracellular polymers in activated sludge,” Water Science and Technology, 2001, 44(10), 221-229.

Edzwald, J. K., and Tobiason, J. E., “Enhanced coagulation: US requirements and a broader view,” Water Science and Technology, 1999, 40(9), 63-70.

Eckenfelder WW, Jr., “Point toxics control for industrial wastewaters,” Civil Engng Prac, 1988, 3, 98–112.

Furumai, H., and Rittmann, B. E., “Advanced Modeling of Mixed Populations of Heterotrophs and Nitrifiers Considering the Formation and Exchange of Soluble Microbial Products,” Water Science and Technology, 1992, 26(3-4), 493-502.

Fearing, D.A., Goslan, E.H., Banks, J., Wilson, D., Hillis P., Campbell, A.T. and Parsons, S.A., “Staged coagulation for treatment of refractory orgasnics,” Journal of Environmental Engineering-Asce, 2004, 130(9), 975-982

Hilal, N., Al-Zoubi, H.; Mohammad, A. W., and Darwish, N. A., “Nanofiltration of highly concentrated salt solutions up to seawater salinity,” Desalination 2005, 184(1-3), 315-326.

John E., James K.,“Chemical aspects of coagulation using aluminum salts—I. Hydrolytic reactions of alum and polyaluminum chloride,” Water Research, 1990 24(12), 1519-1526.

Jarusutthirak, C., and Amy, G.,“Role of soluble microbial products (SMP) in membrane fouling and flux decline,” Environmental Science & Technology, 2006, 40(3), 969-974.

Kudermann, G., Blaufuss, K. H., Luhrs, C., Vielhaber, W., and Collisi, U., “Ultra Trace Analysis of High-Purity Aluminum. Fresenius,” Journal of Analytical Chemistry, 1992, 343(9-10), 734-740.

Kuo W. C. “Production of soluble microbial chelators and their impact on anaerobic treatment,” Ph.D.thesis, 1993 University of Iowa, Iowa City

Koros, W. J., Ma, Y. H., and Shimidzu, T., “Terminology for membranes and membrane processes,” Journal of Membrane Science, 1996, 120(2), 149-159.

Kuo, W. C., Sneve, M. A., and Parkin, G. F., “Formation of soluble microbial products during anaerobic treatment,” Water Environment Research, 1996, 68(3), 279-285.

Laspidou, C. S., and Rittmann, B. E., “A unified theory for extracellular polymeric substances, soluble microbial products, and active and inert biomass,” Water Research, 2002, 36(11), 2711-2720.

Leung, ”Effect of extracellular polymeric substances (EPS) of activated sludge and diatom blooms on their bioflocculation behaviour,” MS Thesis, 2003, Hong Kong University, Hong Kong.

Li X. Y., Wang H. W., Leung RPC, “Influence of loosely-bound extracellular polymeric substances on flocculation and sedimentation of activated sludge,” International Symposium on Biotechnology for Environmental Pollution Control, Beijing, China, 2004, p.191-196.

Liu, H., and Fang, H. H. P., ”Extraction of extracellular polymeric substances (EPS) of sludges,” Journal of Biotechnology, 2002, 95(3), 249-256.

Liu, Y., Yang, S. F., Liu, Q. S., and Tay, J. H., “The role of cell hydrophobicity in the formation of aerobic granules,” Current Microbiology, 2003, 46(4), 270-274.


Logan, B. E., and Qing, J., “Molecular-Size Distributions of Dissolved Organic-Matter,” Journal of Environmental Engineering-Asce, 1990, 116(6), 1046-1062.

Mahmoud, N., Zeeman, G., Gijzen, H., and Lettinga, G., “Solids removal in upflow anaerobic reactors, a review,” Bioresource Technology, 2003, 90(1), 1-9.

Magbanua, B. S., and Bowers, A. R., “Characterization of soluble microbial products (SMP) derived from glucose and phenol in dual substrate activated sludge bioreactors,” Biotechnology and Bioengineering, 2006, 93(5), 862-870.
Namkung, E., and Rittmann, B. E., “Soluble Microbial Products (Smp) Formation Kinetics by Biofilms,” Water Research, 1986, 20(6), 795-806.

Namkung, E., and Rittmann, B. E., “Effects of Smp on Biofilm-Reactor Performance,” Journal of Environmental Engineering-Asce, 1988, 114(1), 199-210.

Nelson, Y. M., Lion, L. W., Shuler, M. L., and Ghiorse, W. C., “Modeling oligotrophic biofilm formation and lead adsorption to biofilm components,” Environmental Science & Technology, 1996, 30(6), 2027-2035.

Noguera, D. R., Araki, N., and Rittmann, B. E., “Soluble Microbial Products (Smp) in Anaerobic Chemostats,” Biotechnology and Bioengineering, 1994, 44(9), 1040-1047.

Nielsen, P. H.; Jahn, A.; Palmgren, R., Conceptual model for production and composition of exopolymers in biofilms. Water Science and Technology 1997, 36, (1), 11-19.

Poxon, T. L., and Darby, J. L., “Extracellular polyanions in digested sludge: Measurement and relationship to sludge dewaterability,” Water Research, 1997, 31(4), 749-758.

Park, N., Kwon, B., Kim, I. S., and Cho, J. W., “Biofouling potential of various NF membranes with respect to bacteria and their soluble microbial products (SMP): Characterizations, flux decline, and transport parameters,” Journal of Membrane Science, 2005, 258(1-2), 43-54.

Rappaport, S. M., Richard, M. G., Hollstein, M. C., and Talcott, R. E., “Mutagenic Activity in Organic Wastewater Concentrates,” Environmental Science & Technology, 1979, 13(8), 957-961.

Ramesh, A., D.J. Lee, and S.G. Hong, “Soluble microbial products (SMP) and soluble extracellular polymeric substances (EPS) from wastewater sludge,” Applied Microbiology and Biotechnology, 2006, 73(1), 219-225.

Rouxhet, P. G., and Mozes, N., “Physical-Chemistry of the Interface between Attached Microorganisms and Their Support,” Water Science and Technology, 1990, 22(1-2), 1-16.

Shon, H. K., Guo, W. S., Vigneswaran, S., Ngo, H. H., and Kim, I. S., “Effect of flocculation in membrane-flocculation hybrid system in water reuse,” Separation Science and Technology, 2004, 39(8), 1871-1883.

Shen, C. F., Kosaric, N., and Blaszczyk, R., “Properties of Anaerobic Granular Sludge as Affected by Yeast Extract, Cobalt and Iron Supplements,” Applied Microbiology and Biotechnology, 1993, 39(1), 132-137.

Schmidt, J. E., and Ahring, B. K., “Extracellular Polymers in Granular Sludge from Different Upflow Anaerobic Sludge Blanket (Uasb) Reactors,” Applied Microbiology and Biotechnology, 1994, 42(2-3), 457-462.

Tchobanoglous, G., Darby, J., Bourgeous, K., McArdle, J., Genest, P., and Tylla, M., “Ultrafiltration as an advanced tertiary treatment process for municipal wastewater,”. Desalination 1998, 119(1-3), 315-321.

Urbain, V., Mobarry, B., de Silva, V., Stahl, D. A., Rittmann, B. E., and Manem, J., “Integration of performance, molecular biology and modeling to describe the activated sludge process,” Water Science and Technology, 1998, 37(4-5), 223-229.

Vanbenschoten, J. E., and Edzwald, J. K., “Measuring Aluminum during Water-Treatment - Methodology and Application,” Journal American Water Works Association 1990, 82(5), 71-78.

Warner, I. M., Callis, J. B., Davidson, E. R., and Christian, G. D., “New Data Reduction Strategies for Multicomponent Fluorescence Analyses,” Abstracts of Papers of the American Chemical Society 1976, 172(Sep3), 26-26.

Wingender, J., Neu, T.R., and Flemming, H.C., “Microbial　Extracellular Polymeric Substances: Characterization, Structures and Function,” Springer-Verlag, 1999, Berlin Heidelberg，Chapter 3.

Wang L., Song,L., “Flux decline in crossflow microfiltration and ultrafiltration experimental verification of Fouling Dynamics,” Journal of Membrane Science, 2003 160 ,p.41~50