臺灣博碩士論文加值系統

麻薏 (Corchorus capsularis) 為黃麻之嫩葉，本研究探討麻薏不同部位其萃取物的抗氧化力和調控發炎反應之能力。實驗中利用Trolox 當量濃度來換算其抗氧化之效果，結果顯示麻薏碎葉乙醇萃取物 (CGLEE) 經細胞處理後，經換算其Trolox濃度為4.12 μM，表示該樣品有較佳的抗氧化功效。
在短暫轉染實驗中，則以麻薏葉脈熱水萃取物 (CVHWE) 91.6％活化PPARγ效果最明顯。在細胞存活率方面，麻薏各萃取物在100 μg/mL之濃度下與巨噬細胞共同培養後其細胞存活率均無顯著差異(p＞0.05)。抑制經由脂多醣誘導巨噬細胞RAW264.7細胞生成NO2之表現的能力，麻薏完整葉乙醇萃取物 (CCLEE)、麻薏含葉脈熱水萃取物 (CLCVHWE) 均能顯著抑制LPS所誘導RAW264.7細胞生成NO2之作用，而麻薏葉去葉脈熱水萃取物 (CLRVHWE) 則無效果。
在PGE2生成結果中，發現麻薏完整葉乙醇萃取物 (CCLEE) 在減少PGE2之生成量效果最佳，且有統計上之顯著差異；而在麻薏熱水萃取物部份則無差異。對於LPS誘導RAW264.7巨噬細胞株COX-2蛋白質之表現情形，以麻薏碎葉乙醇萃取物 (CGLEE) 降低COX-2蛋白表現效果最為顯著 (p＜0.05)，在麻薏水洗葉部分雖然抑制COX-2蛋白質之表現，但其PGE2之生成量與控制組相當。
對於NF-κB蛋白質表現上，與控制組相比麻薏莖甲醇萃取物 (CSME)、麻薏含葉脈熱水萃取物 (CLCVHWE) 有顯著抑制NF-κB p65蛋白質之表現。綜合上述結果，以麻薏碎葉乙醇萃取物 (CGLEE) 抑制NO2、PGE2生成及抑制COX-2蛋白表現有顯著功效，且具有降低NF-κB蛋白質表現之趨勢。

This study was discussed the different parts of Corchorus capsularis, and their antioxidant activity and the ability on the inflammatory effects by LPS-activated macrophage cell line. In the antioxidant activity, CGLEE had the best suppression. In transient transfection assay showed that the CVHWE activated PPARγ significantly. The MTT assay results showed that Corchorus capsularis extracts treatments did not significantly influence the cell viability. However, the Corchorus capsularis leaf reject vein hot water extract (CLRVHWE) had no effect. The CCLEE could inhibited the PGE2 synthesis, and Corchorus capsularis hot water extract had no significantly difference. In addition, suppression on LPS-induced COX-2 protein expression of RAW264.7 showed that CGLEE was the best. The CLCVHWE and CLRVHWE inhibited COX-2 protein expression, but PGE2 had no different with vehicle. In the NF-κB p65 protein expression, the CSME and CLCVHWE had significant ability inhibition. In conclusion, the effects of CGLEE could suppressed NO2, PGE2 and COX-2 protein expression and decreased NF-κB p65 protein expression.

目錄………………………………………………………………………ⅰ
表目錄…………………………………………………………………iii
圖目錄………………………………………………………………… iv
中文摘要…………………………………………………………………v
英文摘要…………………………………………………………………vi
樣品名稱縮寫對照表………………………………………………… vii
第一章 緒論…………………………………………………………… 1
第二章 文獻探討……………………………………………………… 3
第一節 麻薏簡介……………………………………………………… 3
第二節 發炎反應……………………………………………………… 8
一、 巨噬細胞…………………………………………………… 8
二、 脂多醣體…………………………………………………… 8
三、 一氧化氮…………………………………………………… 10
四、 NF-kB……………………………………………………… 11
五、 前列腺素…………………………………………………… 16
第三節 過氧化體增殖劑活化受器…………………………………… 18
一、 過氧化體…………………………………………………… 18
二、 過氧化體增殖劑活化受器介紹…………………………… 18
第四節 酚類化合物…………………………………………………… 24
第三章 材料與方法………………………………………………… 26
第一節 實驗架構……………………………………………………… 26
第二節 實驗材料……………………………………………………… 27
第三節 麻薏樣品萃取製備方法……………………………………… 29
一、 麻薏莖、葉有機溶劑萃取物……………………………… 30
二、 麻薏葉熱水萃取物………………………………………… 31
第四節 實驗分析步驟………………………………………………… 32
一、 細胞培養…………………………………………………… 32
二、 細胞存活率分析…………………………………………… 32
三、 短暫轉染…………………………………………………… 33
四、 NO2分析…………………………………………………… 33
五、 前列腺素E2分析…………………………………………… 34
六、 西方墨點法分析…………………………………………… 35
七、 抗氧化分析………………………………………………… 36
八、 質體製備…………………………………………………… 37
第四章 結果………………………………………………………… 39
第五章 討論…………………………………………………………… 51
第六章 結論…………………………………………………………… 55
參考文獻……………………………………………………………… 56

表目錄

表一、圓果種與長果種黃麻之差異...................................................................... 4
表二、為日本甜麻葉營養成份分析和其他蔬菜營養價值之比較........................ 6
表三、葉用黃麻成分分析表.................................................................................. 7
表四、麻薏各萃取物之產率................................................................................ 43

圖目錄
圖一、脂多糖體之結構............................................................................................ 9
圖二、TNF-影響NF-B活性途徑........................................................................ 13
圖三、經誘發NF-B使基因產生轉錄活化作用而抑制細胞凋亡機制................ 13
圖四、蛋白分解系統活化NF-B機制................................................................... 14
圖五、活化NF-B五大種類因子在細胞中的表現............................................... 15
圖六、PGE2 生成途徑............................................................................................ 17
圖七、PPAR結構圖................................................................................................. 19
圖八、PPAR異構體................................................................................................ 23
圖九、類黃酮之基本結構....................................................................................... 25
圖十、麻薏莖、葉之乙醇萃取流程圖................................................................... 30
圖十一、麻薏葉熱水萃取流程圖............................................................................ 31
圖十二、麻薏各萃取物對RAW264.7細胞存活率之影響..................................... 44
圖十三、麻薏各萃取物對LPS誘發巨噬細胞RAW264.7細胞之NO2生成影響...45
圖十四、麻薏各萃取物對PPAR 促效劑之相對活化倍數.................................. 46
圖十五、麻薏各萃取物對 LPS 誘導RAW264.7細胞生成 PGE2之影響........... 47
圖十六、麻薏各萃取物對LPS 誘發巨噬細胞COX-2蛋白質表現之影響........ 48
圖十七、麻薏各萃取物對巨噬細胞NF-κB蛋白質表現之影響........................... 49
圖十八、麻薏各萃取物之抗氧化能力.................................................................... 50

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