臺灣博碩士論文加值系統

本研究主要是以隨機增殖聚合酵素連鎖反應 (RAPD PCR) 方法進行犬
之DNA指紋圖譜分析，所分析的犬種包括台灣犬 (Formosan dog)、德國狼
犬 (German Shepferd)、柴犬 (Shiba Inu)、比特犬 (Pit bullterrier)
、秋田犬 (Akita)、可卡犬 (Cocker Spaniel)及雜種犬 (mixed-breed
dog)。利用隨機合成之核酸引子，分別對此六種品種及雜種犬之DNA混合
溶液進行聚合酵素連鎖反應，由180種核酸引子中篩選出其中二十種反應
較穩定之引子進行分析，共得到210個DNA片段，每一引子在各品種間增殖
出2∼9個相同之DNA片段，故共得到118在六個品種及雜種犬間共有之特定
的DNA片段，構築出此六品種及雜種犬之DNA指紋圖譜。所得到之DNA片段
中有43.8%並非此六品種犬及雜種犬所共有的多形性 (polymorphic) 產物
。經distance matrix methods分析此六品種及雜種犬間之相關性，結果
顯示每個品種間之平均相關係數為0.704至0.847。此外，亦以其中十四種
十基核酸引子進行此六個品種及雜種犬的個體試驗，結果發現，大部分產
物均出現於各品種之個體，但有部分產物在各品種間顯示有很大之差異性
。

We established DNA fingerprints and assayed the correlation
of seven dog breeds which included Formosan dog, German
Shepherd, Shiba Inu, Pit bullterrier, Akita, Cocker Spaniel, and
mixed-breed dog by random amplified DNA polymerase chain
reaction (RAPD PCR). Twenty primers were selected from breed-
specific DNA pools by screening 180 different ten-base
oligonucleic primers. Analysis of the specific products was
obtained. 43.8% of the specific products were polymorphic. The
correlation index assayed by distance matrix methods between six
breeds and mixed-breed dog was from 0.704 to 0.847. In addition,
applicationof the selected primers to analyzed the individual
DNA of 40 dogs within the six breeds and mixed-breed dogs by
RAPD PCR revealed that some of the amplified products between
those breeds were different. These results suggested that DNA
fingerprints established by RAPD PCR can be served a potential
reference to identify dog breed.