臺灣博碩士論文加值系統

本研究是以薑醇為主體架構，與不同的羥基苯甲酸合成出四個薑醇衍生物，並針對抑制酪胺酸酶活性與抗氧化等能力測試，藉此確認薑醇及其衍生物不同官能基間的活性差異。
薑醇在抑制酪胺酸酶的活性上，於最高濃度10 mM下，無明顯的抑制活性，對照組β-熊果素之抑制酪胺酸酶活性IC50為5.48 mM。抗氧化活性部分，ABTS與DPPH試驗之IC50分別為102.84及73.13 μM。對照組維生素C在抗氧化活性上IC50則為195.57與89.78 μM，顯示出薑醇在抗氧化活性上具有不錯的表現。
所合成出的薑醇衍生物中，薑醇與β-雷鎖酸所合成的衍生物具有最佳的抑制酪胺酸酶活性，其IC50為4.41 mM，屬非競爭型抑制劑。而薑醇與龍膽酸所合成的衍生物，其在抗氧化活性上具有最佳表現，ABTS與DPPH之IC50分別為70.97與47.95 μM。顯示出薑醇透過與羥基苯甲酸的合成，不僅改善薑醇在FRAP之還原能力，還增強了自由基清除活性。特別的是，其在抑制酪胺酸酶的活性上，得到明顯的提升。顯示出此類型酯化合物具有未來作為酪胺酸酶抑制劑與抗氧化劑之潛力。

In this study, we synthesized four zingerol derivatives from hydroxybenzoic acids and evaluated their tyrosinase inhibition and antioxidant activities in order to determine the bioactivity of zingerol and the four derivatives.
Zingerol shows no significant inhibitory effect on mushroom tyrosinase in 10 mM concentration. IC50 value of tyrosinase inhibition of β-arbutin used as a control group is 5.48 mM. Zingerol has great antioxidant activities and the IC50 values of ABTS and DPPH are 102.84 and 73.13 μM, respectively. Vitamin C is used as a control group and the IC50 values are 195.57 and 89.78 μM, respectively.
Among the synthetic zingerol derivatives, the zingerol derivative from β-resorcylic acid performs the best inhibitory effect on tyrosinase and the IC50 value is 4.41 mM. It is a non-competitive inhibitior. Further, the synthetic zingerol derivative from gentisic acid demonstrates the best antioxidant activity and the IC50 values are 70.97 and 47.95 μM, respectively. It means that zingerol derivatives from hydroxybenzoic acids not only improve the activity of zingerol in FRAP assay but also increase the antioxidant capacity in free radical scavenging. It is predicted that the zingerol derivatives have the potential to use as tyrosinase inhibitors and antioxidants in the skin care products.

致謝 I
中文摘要 III
英文摘要 IV
目錄 V
表目錄 VI
圖目錄 VII
緒論 1
結果與討論 15
結論 32
材料與方法 33
參考文獻 52
附錄 56

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