臺灣博碩士論文加值系統

高解析度全域式光學同調斷層顯微術 (Full-Field Optical Coherence Microscopy, FF-OCM)，是一種利用低同調斷層掃描干涉取得三維樣品之技術，相較於其他種類的OCT，最大的不同在於全域式OCT一次掃描即可擷取一張 X-Y平面影像，不需做掃描即可獲得影像，而此種做法用於血管網路成像與細胞運動捕捉具有快速取得大視野的優勢。在本論文中FF-OCM採用微分演算法，不需要使用精確的相位掃描，四張影像即可解調，在速度提升上有很大的幫助。本系統使用supercontinuum laser source (450-1000nm)，中心波長為 650 nm，半高寬為 240 nm，系統測試軸向解析度與橫向解析度分別為0.9 m 和 1.33 m (NA=0.3)，我們搭配三種捕捉動態樣本之演算法用來評估高解析度成像速率之FF-OCM用於之流體成像(速度每秒達50張)。初步測試結果顯示FF-OCM系統可偵測微流道內血液流動達25mm/min之速度與細胞運動之監測。

High-resolution Optical Coherence Microscopy (FF-OCM), based on low-coherence tomography interferometry, is a technique for obtaining sections from three-dimensional samples. Compared with other types of optical coherence tomography (OCT), the most significant difference is that the FF-OCM no need to scan point by point laterally. This X-Y imaging capability is especially important to vascular network imaging and cell dynamics observation with the advantage of rapid access to a large field of view. In this thesis, FF-OCM using derivative demodulation method can get the enface map (X-Y image) using four phase-shifting step. Our system used a supercontinuum laser source with a wavelength range from 450nm to 1000nm. The center wavelength of the laser is 800nm, bandwidth is 300nm, which provides 0.9m axial resolution in air, and 1.33 m lateral resolution by using an objective (NA=0.3). Moreover, we assessed the feasibility of dynamic imaging by the implementation of three calculation methods to this high-resolution FF-OCM for flow mapping with an imaging rate of 50 Hz. Here, we demonstrate the mapping of blood flowing in a microchannel at speeds up to ~ 25 mm/min, and detect the cell motility by using a high-speed, high-resolution FF-OCM setup.

致謝 i
中文摘要 iii
英文摘要 iv
目錄 v
圖目錄 1
表目錄 3
第 1 章 緒論 4
1-1 研究背景 4
1-2 文獻回顧 4
1-2-1 OCT 系統應用於流體影像 4
1-2-2 FF-OCM 應用發展 7
1-3 研究動機與目的 14
第 2 章 背景理論 16
2-1 光學同調斷層掃描術簡介 16
2-2 光學同調斷層掃描術 18
2-3 時域光學同調斷層掃描 (Time Domain OCT, TDOCT) 19
2-4 全域式光學同調斷層掃描術 20
2-5 低同調斷層掃描術理論 21
2-6 干涉可見度 25
2-7 光學同調斷層解析度 25
2-7-1 橫向解析度 26
2-7-2 軸向解析度 28
2-8 視野範圍 29
2-9 相機雜訊特性 30
2-10 靈敏度 31
2-11 動態範圍(Dynamic range) 32
2-12 全域式 OCT 之重建影像演算法 32
2-12-1 微分演算法 33
2-13 血管影像對比增強演算法(IBDV)介紹 34
2-14 雷射散斑波動和運動幅度分析 36
第 3 章 系統架構與參數 38
3-1 Linnik-base FFOCM架構與介紹 38
3-2 干涉可見度 40
3-3 橫向解析度 41
3-4 軸向解析度 43
3-5 視場範圍 45
3-6 相機規格和雜訊特性 45
3-7 動態範圍、靈敏度 46
3-8 微分演算法 47
第 4 章 影像重建與結果討論 51
4-1 實驗樣品 51
4-2 微流道仿體流動影像擷取 52
4-3 微流道仿體影像三種演算法最佳化參數 53
4-4 IBDV 影像最佳化參數 54
4-4-1 IBDV 影像平均張數 54
4-4-2 IBDV 影像延遲時間測試 56
4-4-3 IBDV影像延遲時間測試於血管模擬 58
4-4-4 IBDV影像流速測試於血管模擬 61
4-5 STD影像最佳化參數 63
4-5-1 STD 影像平均張數 63
4-5-2 STD 影像延遲時間測試 65
4-5-3 STD 影像延遲時間測試於血管模擬 67
4-5-4 STD 影像流速測試於血管模擬 68
4-6 SFA 影像最佳化參數 69
4-6-1 SFA 影像平均張數測試 69
4-6-2 SFA影像延遲時間測試 71
4-6-3 SFA 影像延遲時間測試於血管模擬 72
4-6-4 SFA影像流速測試於血管模擬 73
4-7 微流道仿體之 OCT 影像與 IBDV、STD與SFA影像動態評估 74
4-8 IBDV、STD與SFA 應用於細胞動態評估 77
4-9 捕捉細胞動態影像 79
第 5 章 結語與未來展望 80
參考文獻 81

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