臺灣博碩士論文加值系統

本研究嘗試利用微弧氧化法製備含銀之氧化鈦層，本論文分為兩部分：第一部分以微弧氧化法製備氧化鈦層。第二部分嘗試使用多種方法使銀附著於氧化鈦層表面，並以掃描式電子顯微鏡(SEM)觀察不同方法之表面形貌、能量發散光譜儀(EDS)觀察表面成分、微細形狀測定儀(α-step)分析表面粗糙度及膜厚、X光繞射儀(XRD)觀察微弧氧化層的結構及相組成，最後使用MTT法檢測比較含銀之氧化鈦層表面細胞活性及抗菌效果。第一部分研究實驗結果顯示：本實驗已成功製備晶相為金紅石及銳鈦礦且具有孔洞之氧化鈦層，且氧化鈦層之厚度、表面孔洞大小、粗糙度及親水性會隨著反應電壓增高而隨之提高。第二部分研究結果顯示：先進行微弧氧化法製備氧化鈦層後再進行銀的還原處理，由此方法可使銀粒子附著於氧化鈦層表面，在經過生物活性檢測後發現含銀粒子之氧化鈦層生物活性會低於未含銀之氧化鈦層，但兩者之生物活性都具有較佳之生物相容性。再經由細菌培養後，有添加銀之氧化鈦層周圍細菌量開始減少，且含銀量越高周圍的細菌量越少。

This study attempted to utilize microarc oxidation to prepare titanium oxide layer containing silver. The present paper was divided into two parts: the first part was to prepare titanium oxide layer via microarc oxidation. The second part tried to use various approaches for adhering silver to the surface of titanium oxide layer. Field Emission Scanning Electron Microscopy (SEM) was used to observe the surface morphology produced by different approaches. Energy Dispersive Spectrometer (EDS) was employed to observe surface composition, whereas Micro-Shape Analyzer (α-step) for analyzing surface roughness and thickness and X-ray generator (XRD) for observing structure and phase composition of microarc oxidation layer. Finally, the MTT assay was conducted to compare the cell activity and antibacterial effects of Titanium Oxide layer containing silver. Research findings of the first part showed that: the experiment successful prepared the crystalline phase of rutile and anatase with pores in the titanium oxide layer, in which the thickness, size of surface pore, roughness and hydrophilic of titanium oxide will be elevated as the reaction voltage increased. Results of the second part of the study indicated that: the silver reduction treatment method on titanium oxide layer prepared by microarc oxidation could adhere silver particles to the surface of the titanium oxide layer. After bioassay, it was found that the biological activity of titanium oxide layers containing silver particles was lower than that of titanium oxide layer without containing silver, but both biological activities hadbetter biocompatibility. Throughout bacterial culture, the quantity of bacteria around titanium oxide layer with the addition of silver began to reduce, and the higher the silver content resulted in the less the quantity of bacteria around.

摘 要 i
Abstract ii
誌 謝 iv
目 錄 v
圖目錄 viii
表目錄 xi
第一章 緒論 1
1.1 前言 1
1.2 研究動機及目的 3
1.3 研究架構 4
第二章 文獻回顧與理論 6
2.1 鈦與鈦合金及其氧化物 6
2.2 氧化鈦薄膜的製備 10
2.2.1 溶膠-凝膠法 10
2.2.2 氣相沉積法 11
2.2.3 陽極氧化法 13
2.2.4 微弧氧化法 17
2.3 氧化鈦薄膜抗菌的應用 21
第三章 實驗方法 25
3.1 實驗步驟 25
3.1.1 試片表面前處理 25
3.1.2 微弧氧化處理 26
3.1.3 實驗條件 28
3.2 氧化膜分析及檢測 29
3.2.1 表面形貌分析 29
3.2.2 成分分析 30
3.2.3 鍍層晶相分析 31
3.2.4 膜層厚度測量 32
3.2.5 膜層親水性測試 33
3.2.6 膜層表面粗糙度分析 34
3.2.7 酵素免疫微量盤分析儀 35
3.3 體外細胞培養 36
3.4 抗菌培養實驗 40
3.5 實驗材料及反應設備 41
3.5.1 材料 41
3.5.2 反應設備 41
第四章 結果與討論 42
4.1 微弧氧化鈦層的製備(第一階段) 42
4.1.1 表面形貌分析 42
4.1.2 鍍層晶相分析 45
4.1.3 粗糙度分析與水滴接觸角分析 46
4.2 添加抗菌劑對氧化鈦膜之影響(第二階段) 48
4.2.1 方法A之實驗結果 49
4.2.2 方法B之實驗結果 52
4.2.3 方法C之實驗結果 58
4.2.3.1 表面形貌分析 59
4.2.3.2 鍍層表面分析 63
4.2.3.3 成分分析 64
4.2.3.4 粗糙度分析與水滴接觸角分析 66
4.3 細胞培養實驗結果 69
4.4 細菌培養實驗結果 72
第五章 結論與未來建議 76
5.1 結論 76
5.2 未來建議 77
參考文獻 78

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