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Recently, the polyphenol compounds containeds in natural plants
which were researched in antivirus to consider important.
Taiwan is located in the subtropical zone and there are
aboundant plants in Taiwan.Using chemical methods to screen
Taiwan folk medicines which contained polyphenols and anti-EBV
(Epstein-Barr virus) DNA polymerase activity in vitro which is
associated with nasopharyngeal carcinoma (NPC). Eugenia
uniflora Linn. is one of the active folk medicines. By applied
of various chromatographic and spectroscopic techniques to the
isolation, analysis and structure determination of these
compounds, such as: column chromatography、high-performance
liquid chromatography (HPLC)、 FAB-MS、UV spectra、IR spectra、1
H-NMR spectra、13C-NMR spectra、 CD spectra and so on. There
were four crystal compounds, which were isolated from ethyl
acetate fraction of the Eugenia uniflora, gallocatechin and
myricitrin, are testing for anti-EBV DNA poly- merase activity.
In addition the mechanism of activity was used the cell lines
(eg: Raji cells) to do further study. Another research is using
HPLC method to quantity of gentio- picroside which is the
conducive component to the health of stomach. The best yield of
the Long-Dan in different extraction conditon was
investigated. In addition, in various drug markets and the
various contents of gentiopicroside in the Chinese medicinal
prescriptions containing Long-Dan were also performed. The
results were found 100 volume of Long-Dan(W/V) of 50﹪ methanol
in 50 minutes of sonication could produce the best yield. The
contents of gentiopicroside in various drug markets were found
in range from 1.30﹪ to 5.86﹪.
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