臺灣博碩士論文加值系統

磷脂類與Cholesterol是生物細胞膜的重要成份之一，磷脂類在適當條件下，可形成類似細胞膜的雙層結構；DPPC是常見的磷脂類之一，因此是研究細胞膜性質的極佳對象。而Cholesterol會對磷脂類產生某些交互作用，而有減低或增強細胞膜流體性(fluidity)的情形出現。在本論文中，我們藉由架設一套Micro-Raman系統，研究不同碳鏈長的PC類分子在熱力學行為上之差異，從Raman散射所提供的分子振動態之訊息，來了解不同溫度下其分子性質產生的變化，並觀測Cholesterol的存在對DPPC所造成的影響。

壹 簡介
1.1.細胞膜脂質分子……………………………………………….1
1.2.Raman散射…………………………………………………….3
貳 量測儀器及原理
2.1.單光儀…………………………………………………………15
2.2.CCD……………………………………………………………20
參 實驗設置及步驟
3.1.實驗設置………………………………………………………35
3.1.1光路設置…………………………………………………35
3.1.2.儀器、元件規格…………………………………………37
3.2.實驗步驟………………………………………………………39
3.2.1.樣品準備…………………………………………………39
3.2.2. CCD spectrum校正……………………………………...42
3.2.3.Raman訊號量測…………………………………………44
肆 實驗結果與討論
4.1.系統測試………………………………………………………63
4.2.脂質分子的研究………………………………………………65
4.2.1.PC類與Cholesterol分子振動態介紹…………………..65
4.2.2.PC類分子系統…………………………………………..68
4.2.3.Cholesterol分子系統…………………………………….71
4.2.4.DPPC與Cholesterol分子混合系統…………………….73
伍 結論………………………………………………………101
參考文獻……………………………………………………..104

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