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1990年 Williams等人提出了一個聚合鎖反應的新方法。這個由傳統PCR
為基礎所衍生出來的技術，只使用一個由隨意序列所組成、長為十個鹼基
的引子，而隨機增殖任何基因組不同基因位置之DNA片段，因而被稱為隨
機增殖聚合鏈鎖反應。 我們利用這最新發展出來的方法，來試驗
RAPD技術是否適用於球蟲品種鑑定及不同族群的區分。同時也分析一些可
能由DNA、鎂離子、引子、去氧核糖三磷酸及Taq聚合等PCR反應物所造
成的人為變異。兩種未芽胞化的球蟲， Eimeria tenella 及 Isospora
mikei，經 RAPD-PCR反應後，分析得到46及39個RAPD markers 而成功地
區分這兩種球蟲。此外，由11株Eimeria tenella 以12個引子經增殖作用
總共產生 78個特定的DNA片段。不同株之Eimeria tenella 以 binary
data 紀錄特定片段之有或無，並以Sorrensen's similarity
coefficient 計算其相似指數。其結果顯示，RAPD-PCR技術的確適用於
Eimeria tenella 之鑑定。
A polymerase chain reaction PCR-based method, described first
by Williams et al.(19900, employs a single 10 base-long primers
of arbitrary DNA sequence and amplifies DNA fragments of
differ- ent loci from any genome know as random amplified
polymorphic DNA(RAPD). We applied the lately developed
technique to deter- mine if RAPD of coccidium DNA could be used
for the identifi- cation of unknown specimens, and
differentiation of population. The artificial variation of the
origns of the DNA extract, mag- nesium, primer, dNTPs, and Taq
DNA polymerase was analyzed. Two unsporulated coccidium
coccidium, Eimeria tenella and Isospora mikei, were
differentiated successfully using RAPD markers, which wrer 46
and 39 polymorhpic fragments respec- tively, obtained by the
polymerase chain reaction. On the other hand, a total of 78
reproducibility, distinct fragments of elevent Eimeria tenella
amplified by 12 ten-base primers of arbitrary sequence in the
polymerase chain reaction was pro- duced. Different strains of
Eimeria tenella were scored for presence or absence of RAPD
fragments by binary data method and calculated the similarity
index of the pairwise strains by Sorenson's similarity
coefficient. The results indicate the RAPD-PCR technique will
be useful in study of E. tenella identification.
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