臺灣博碩士論文加值系統

以粒線體DNA中12S核糖體核酸與細胞色素b的部分序列，分析守宮（Gekko hokouensis Pope, 1928）在台灣及東部兩離島，共15個族群間的遺傳距離與親緣關係。以群聚分析法與最檢約法得到之親緣關係樹，可將守宮族群分為五個群：1.西部群、2.礁溪族群、3.東部群、4.東-北部群及5.東-南部群。其中僅礁溪族群的分支位置仍不確定，可能為西部群的一分支。以親緣關係樹的分支情況與遺傳距離推測：東-南部群的蘭嶼族群可能源自恆春半島。另外，以主成分分析法分析15個外部形態特徵的結果，發現除礁溪族群外，其他地區或族群之間皆無法有效區別。最後，完整定序守宮粒線體中的控制區域及其兩側基因部分序列，共計有2865 bp。

Using partial 12S rRNA and cytochrome b sequencing to analyze the population variation and phylogeny of Gekko hokouensis from 15 populations in Taiwan and accessory islands. Constructed by both distance and maximum parsimony methods, the phylogenetic tree distinguish the Gekko hokouensis to 5 clades: 1.Western group; 2.ChaoShi population; 3.Eastern group; 4.Northeastern group; and 5.Southeastern group. The phylogenetic position of ChaoShi population remains ambiguous, which may be one of the Western group. According to phylogenetic tree and genetic distances, I infer that Lanyu population may be originated from HenChuen. Besides the molecular phylogeny, 15 morphological characters were also analyzed by Principal Component Analysis to realize the morphological variation between populations. It reveals that nearly all population are not distinguishable expect the ChaoShi population. Besides above, the control region and the neighboring, the 12S rRNA and cytochrome b genes were also sequenced, total 2865 bp, to be a reference for future study.

壹、前言………………………………………………………… 1
貳、材料與方法………………………………………………… 4
參、結果…………………………………………………………14
肆、討論…………………………………………………………19
伍、結論…………………………………………………………25
陸、參考文獻……………………………………………………26
柒、附錄…………………………………………………………31

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