臺灣博碩士論文加值系統

本研究目的在使用固相微萃取法(Solid phase microextraction,簡稱SPME)為採樣工具，同時結合蟹殼、甲殼素吸附劑和氣體層析儀(Gas chromatography)來處理和偵測奶品和染整廢水。固相微萃取採樣技術具有快速、簡單和不需使用任何有機溶劑等優點，現今已應用於各種分析化學領域。牛乳在加熱處理時，會產生影響其風味的揮發性硫化物，但是硫化物的採樣不易，所以我們採用了頂空固相微萃取法(HS-SPME)的方式採樣，然後再以氣體層析儀連結無火焰硫化學發光偵測器偵測，並以標準品鑑定出所含硫化物, 其中包括硫化氫、甲硫醇、二甲基硫、二甲基二硫和二甲基三硫。在染整廢水的採樣方面，我們使用直接固相微萃法(DI-SPME)採樣其中的污染物，並結合蟹殼和甲殼素吸附劑來處理。其中使用氣體層析儀連結火焰離子偵測器來監測廢水處理前後的差異，接著將偵測到的污染物以氣體層析儀連結質譜儀鑑定出來。

In this study, we use solid phase microextraction(SPME) method as a sampling tool, then treat with the adsorbent of natural crab shell powder, chitin and chitosan, and gas chromatography to analyze the milk and dyeing wastewater. SPME is a rapid, simple, solvent-free and sensitive method of sample preparation. Therefore, SPME is widely used in various analysis chemistry territories. During milk be heated treatment, it will be produced volatile sulfur compounds which contributes significantly to the flavour of milk. Owing to the sulfur compounds is not easy to sampling, we used HS-SPME method to extract them. After the sampling, we inject the sample to the GC-Flameless SCD for analysis. The sulfur compounds were identified by comparing with the retention times of standards and were H2S, CH3SH, DMS, DMDS and DMTS. In sampling of dyeing wastewater, we used the mode of DI-SPME to study the pollutants of wastewater. For wastewater treatment, we also use the natural crab shell powder, chitin and chiosan as the adsorbents. Finally, to monitor the efficiency of wastewater treatment, we use GC-FID and the pollutants are identified by GC-MS.

一、緒論
二、實驗
三、結果與討論
四、結論
五、參考資料

1.林慶文編著，乳品加工學，華香園出版社，台北市 (1991)
2.謝繼志主編，范立冬、趙平副主編，液態乳制品科學與技術，中國輕工業出版社 (1999)
3.G. Gerbersmann, R. Lobinski and F. C. Adams, Anal. Chim. Acta. 316 (1995) 93
4.M. Simon, A.P. Hansen, C.T. Young, J. Dairy Sci. 84 (2001) 774
5.W. Wardencki, J. Chromatogr. A 793 (1998) 1
6.H. Kataoka, H. L. Lord, J. Pawliszyn, J. Chromaogr. A 880 (2000) 35
7.SUPELCO Bulletin 916
8.J. Pawliszyn, “Solid Phase Microextraction Theory and Practice”,WILEY-VCH, New York, (1998)
9.M.A. Farajzadeh, M. Hatami, J. Sep. Sci. 26 (2003) 802
10.楊萬發編著，染整工廠廢水污染防治，經濟部工業局，台北市 (1986)
11.C.I. Pearce, J.R. Lloyd, J.T. Guthrie, Dyes and Pigments 58 (2003) 179
12.X.Z. Li, Y.G. Zhao, Wat. Sci. Tech. 39 (1999) 249
13.高肇藩、張祖恩編著，水污染防治，中國土木水利工程學會，台北市 (1991)
14.陳美惠、莊淑惠、吳志津，食品工業月刊，第31卷第10期 (1999)
15.江晃榮、林玉媛，生物科技的奇蹟甲殼質的強效，世茂出版社，台北縣新店市 (1998)
16.江晃榮、林玉媛，生物科技的奇蹟甲殼質的強效，世茂出版社，台北縣新店市 (2000)
17.徐世昌，化工資訊 (2001) 36
18.林錫杰，食品工業，32 (2000) 27
19.F. Nanjo, K. Sakai, M. Ishikawa, K. Isobe and T. Usui, Agric. Biol. Chem., 53 (8) (1989) 2189
20.陳榮輝，科學發展，第29卷11期 (2001) 776
21.Hackman, R.H. Austr., J. Boil. Sci., 7 (1954) 168
22.幾丁質幾丁聚醣研討會論文集 (2001)
23.幾丁質幾丁聚醣生物科技應用研討會大會手冊及論文，淡江大學 (2002)
24.C. Leck and L.E. Bagander, Anal. Chem., 60 (1998) 1680
25.C. Persson and C. Leck, Anal. Chem., 66 (1994) 983
26.P.P. Deprez, P.D. Franzmann and H.R. Burton, J. Chromatogr., 362 (1986) 9
27.F. Caron and J.R. Kramer, Anal. Chem., 61 (1989) 114
28.R. Simo, J.O. Grimalt and J. Albagies, J. Chromatogr. A., 655 (1993) 301
29.R. Simo, J.O. Grimalt and J. Albagies, Anal. Chem., 68 (1996) 1493
30.E.R. Lingren, D.W. Pershing, D.A. Kinrchgessner and D.C. Drehmel, J. Chromatogr., 585 (1991) 353
31.G. Castello, G. Damato and M. Nicchia, J. Chromatogr., 521 (1990) 99
32.F.L. Eisele and H. Berreaheim, Anal. Chem., 64 (1992) 283
33.J. Radfork-Knoery and G.A. Cutter, Anal. Chem., 65 (1993) 976
34.N. Kishima, Anal. Chem., 58 (1986) 1255
35.G.A. Cutter and T.J. Oatts, Anal. Chem., 59 (1987) 717
36.Y. Yokouchi, H. Bandow and H. Akimoto., J. Chromatogr., 642 (1993) 401
37.S. Jacobsson and O. Folk, J. Chromatogr., 479 (1989) 1
38.大氣中微量污染氣態化合物之分析研究會，清華大學原子科學研究所 (1997)
39.Rosemary Buffington and Michael K. Wilson, Detector for gas chromatography-a practical primer, (1991)
40.P. Schubert, I. Fernandez-Escobar, E. Rosenberg and J.M. Bayona, J. Chromatogr. A., 810 (1998) 245
41.N.G. Johansen and J.W. Birks, Am. Lab., 112 (1991)
42.J.V. Heodly, Biomed. Environ. Mass Sepctrom., 14 (1987) 275
43.A. Stumpf, K. Tolvaj and M. Juhasz, J. Chromatogr. A., 819 (1998) 67
44.J.F. McGaughey and S.K. Gangwal, Anal. Chem., 52 (1980) 2079
45.R.K. Stevens, J.D. Mulik, A.E. Okeeffe and K.J. Krost, Anal. Chem., 43 (1971) 827
46.P.L. Patterson, R.L. Howe and A. Abu-Shumays, Anal. Chem., 50 (1978) 339
47.H.C. Karen Chang and Larry T. Taylor, J. Chromatogr., 517 (1990) 491
48.W.F. Sye, Z.X. Zhao and M.L. Lee, Chromatographia., 33 (1992) 507
49.R.L. Shearer, D.L. O’Neal, R. Rios and M.D. Baker, J. Chromatogr Sci., 28 (1990) 24
50.R.L. Shearer, E.B. Poole and J.B. Nowalk, J. Chromatogr. Sci., 31 (1993) 82
51.P.L. Burrow and J.W. Briks, Anal. Chem., 69 (1997) 1299
52.R.L. Benner and D.H. Stedman, Anal. Chem., 61 (1989) 1268
53.R.L. Benner and D.H. Stedman, Environ. Sci. Technol., 24 (1990) 1592
54.H.P. Tuan, H.G.M. Janssen, Garel A. Cramers, E.M.K. Loo and H. Vlap, J. High. Resol. Chromatogr., 18 (1995) 333
55.W.F. Sye and C.C. Shu, 淡江學報, 34 (1995)
56.Randall L. Shearer, E.B. Poole and J.B. Nowalk, J. Chromatogr. Sci., 31 (1992) 82
57.Randall L. Shearer, Anal. Chem., 64 (1992) 2192
58.Randall L. Shearer, GC ＆ Flameless Sulfur Detection, (1993) 34
59.M. Nedjma and A. Maujcan, J. Chromatogr., 704 (1995) 795
60.Douglas A. Skoog, F. James Holler, Timothy A. Nieman, “Principles of Instrumental Analysis”, Harcourt Burace ＆ Company, U.S.A., (1998)
61.H. Yoshida, A. Okamoto and T. Kataoka, Chem. Eng. Sci., 48 (1993) 2267