臺灣博碩士論文加值系統

由菊科（Compositae）植物白鳳菜甲醇粗抽物以氯仿抽取的分劃層中進行分離及純化，共單離得到九個化合物及一個混合物：docosane (I)、methyl hexadecanoate (Ⅱ)、methyl oleate (III)、methyl linoleate (IV)、friedelin (V)、β-sitosterol及stigmasterol混合物 (VI)、methyl 132-hydroxy- (132-S)-pheophorbide b (VII)、methyl 132-hydroxy- (132-S)- pheophorbide a (VIII)、7,11,15 -trimethyl-3-methylene-1,2- hexadecanediol (IX)、1,2-dihydroxypropyl hexadecanoate (X)。以上九個化合物及一個混合物均為已知的，但其中除了friedelin (V)、β-sitosterol及stigmasterol混合物(VI)外，其餘都是第一次於白鳳菜中分離得到。
經抗細胞增殖試驗結果發現methyl oleate (III)、methyl linoleate (IV)、7,11,15 -trimethyl-3-methylene-1,2-hexadecanediol (IX)有明顯抑制HL-60細胞增殖活性。其餘化合物之抗細胞增殖尚在進行中。
白鳳菜分離所得的化合物之抗過敏、抗發炎活性試驗尚在送試中。

Nine compounds and one mixture- docosane (I)、methyl hexadecanoate (Ⅱ)、methyl oleate (III)、methyl linoleate (IV)、friedelin (V)、β-sitosterol及stigmasterol (VI)、methyl 132-hydroxy-(132-S)- pheophorbide b (VII)、methyl 132-hydroxy- (132-S)- pheophorbide a (VIII)、7,11,15 -trimethyl-3-methylene-1,2- hexadecanediol (IX)、1,2-dihydroxypropyl hexadecanoate (X), have been isolated from the chloroform fraction of the methanolic extract of Gynura divaricata (L.) DC. subsp. formosana (Kitam.) F. G. Davies. Beside compound V friedelin and the mixture of stigmasterol and β-sitosterol (VI), these compounds are isolated for the first time from this plant.
The results of the biological activities show of methyl oleate (III)、methyl linoleate (IV)、7,11,15 -trimethyl-3-methylene-1,2-hexadecanediol (IX) exhibited antiproliferation activities of HL-60 cells.
The of antiinflammatory and antiallergic activities of other compounds are still under test.

目 錄
壹、緒論
第一章 研究背景和目的
第二章 白鳳菜之藥用植物學基原考察
第三章 白鳳菜及同屬植物之成分研究概況
第四章 藥理試驗法簡介
第一節 抗發炎作用
第二節 抗細胞增生作用
貳、結果與討論
第一章 白鳳菜成分之抽取與分離
第二章 白鳳菜成分之化學結構鑑定
第三章 白鳳菜粗抽物與成分之活性
第四章 結論
參、實驗材料與方法
第一章 試藥與儀器
第一節 試藥
第二節 儀器
第二章 成分抽取與分離方法
第一節 預試驗粗抽物之製備
第二節 成分的單離
第三章 藥理試驗方法
第四章 白鳳菜成分之性質與光譜數據
肆、參考文獻
伍、圖譜

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