臺灣博碩士論文加值系統

本研究目的主要探討柴胡疏肝散75%乙醇萃取物對以高膽固醇
飼料誘發高血脂大鼠之降血脂作用。
實驗將雄性SD 大鼠分為六組：正常飼料組、高膽固醇飼料組、
西藥正對照組(高膽固醇飼料+gemfibrozil 250 mg/kg)、柴胡疏肝散
75%乙醇萃取物低劑量組(高膽固醇飼料+柴胡疏肝散3 mg/kg) 、柴胡
疏肝散75%乙醇萃取物中劑量組(高膽固醇飼料+柴胡疏肝散30 mg/kg)
及柴胡疏肝散75%乙醇萃取物高劑量組(高膽固醇飼料+柴胡疏肝散
300 mg/kg)。高膽固醇飼料共計給予四週，第四週後所有動物均給予
正常飼料二週，觀察血清中總膽固醇 (TC)、三酸甘油酯 (TG)、低密
度脂蛋白-膽固醇 (LDL-C)、高密度脂蛋白-膽固醇 (HDL-C)、aspartate aminotransferase (AST)、alanine aminotransferase (ALT)及肝臟中malondialdehyde (MDA)、glutathione peroxidase (GSH-PX)、superoxide dismutase (SOD)、GSH-Px (Glutathione peroxidase)、GSH-Rd(Glutathoine reductase) 的變化來探討柴胡疏肝散降血脂作用。結果顯示高膽固醇飼料組血清中TC、TG、 LDL-C、AST、ALT活性及肝臟中MDA濃度皆有明顯上升，肝臟中抗氧化酵素SOD、GSH-Px、GSH-Rd的活性明顯下降，且高膽固醇飼料組的切片顯示有細胞萎縮、細胞排列不整齊、脂肪空泡等結果，顯示高膽固醇飼料誘具誘發大鼠高脂血症，並造成氧化壓力產生的作用。而在給予柴胡疏肝散75%乙醇萃取物後，血清中TC、TG、LDL-C活性及肝臟中 MDA
濃度皆有明顯降低，且肝臟抗氧化酵素SOD、GSH-Px、 GSH-Rd活
性有明顯的上升，並可降低血清中AST、ALT活性，且在柴胡疏肝散
組中的肝臟組織切片發現細胞萎縮情況減少、脂肪空泡情形降低。證
實柴胡疏肝散75%乙醇萃取物具有降血脂及肝臟保護作用。
綜合以上結果可知柴胡疏肝散75%乙醇萃取物具有降血脂作用，
因其可降低血清中TC、TG、LDL-C 的濃度，且肝臟中MDA 濃度也
有明顯下降。可能為肝中抗氧化酵素SOD、GSH-Px、 GSH-Rd 活性
上升進而降低肝中MDA 及血清中TC、TG、LDL-C 的濃度，使達到
降血脂及肝保護作用。

The aim of the study was intended to evaluate the hypolipidemic effect of ethanolic extract of Chai-Hu-Shu-Gan-San (abbrev. CSSEtOH) in high-cholesterol diet-induced hyperlipidemic rats.
The male Sprague-Dawley rats were divided into standard pellet diet (normal control group), high- cholestetol diet (HFD group), HFD plus gemfibrozil (HFD + Gem group), HFD plus CSSEtOH 3 mg/kg (HFD + CSSEtOH 3 mg/kg group), HFD plus CSSEtOH 30 mg/kg (HFD + CSSEtOH 30 mg/kg group) and HFD plus CSSEtOH 300 mg/kg (HFD + CSSEtOH 300 mg/kg group). The male Sprague-Dawley rats were fed with high-cholesterol diet for 4 weeks. The levels of serum total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C) , aspartate aminotransferase (AST), Alanine aminotransferase (ALT) and hepatic malondialdehyde (MDA), the activities of glutathione peroxidase (GSH-PX) and superoxide dismutase (SOD), GSH-Px (Glutathione peroxidase), GSH-Rd(Glutathione reductase) in the liver were measured.
The HFD rats showed significant increases in the levels of TC, TG, LDL-C, AST, ALT in serum and MDA in hepatic tissue. The activities of SOD, GSH-Px and GSH-Rd were decreased in HFD rats. The CSSEtOH treated rats showed significant decreases in the levels of TC, TG, LDL-C, AST, ALT in serum and MDA in hepatic tissue. CSSEtOH increased the activities of SOD, GSH-Px, GSH-Rd in hepatic tissue,too.
From the above results, it was evident that CSSEtOH possessed hypolipidemic effect. The hypolipidemic mechanism of CSSEtOH may be due to decreasing the levels of TC, TG, LDL-C, AST and ALT in the serum, and MDA in the hepatic tissue and increasing the activities of SOD, GSH-Px, GSH-Rd in the HFD rats.

目錄...................................................... I
謝辭..................................................... IV
中文摘要 ................................................ VI
Abstract .............................................. VIII
縮寫表 ................................................... X
表目錄 ................................................. XII
圖目錄................................................. XIII
第一章 緒論............................................... 1
第二章 總論............................................... 5
第一節 柴胡疏肝散文獻考察 ................................ 5
一、柴胡疏肝散之方劑學考察 ............................... 5
二、柴胡疏肝散組成之成分文獻.............................. 6
三、柴胡疏肝散及其組成之現代研究文獻 .................... 13
第二節 高血脂的中西醫論述及文獻考察 ..................... 28
一、脂質的吸收、合成及代謝............................... 28
二、西藥治療 ............................................ 37
三、中醫臨床常用於治療高脂血症的中藥有 .................. 38
第三節 抗氧化相關文獻 ................................... 39
一、 氧化壓力與高血脂之關係 ............................. 39
第三章 實驗材料和方法 ................................... 47
第一節 實驗架構流程圖 ................................... 47
第二節 實驗材料 ......................................... 48
第三節 實驗動物 ......................................... 53
第四節 柴胡疏肝散急性毒性試驗 ........................... 54
第五節 動物實驗方法 ..................................... 55
第六節 肝臟切片 ......................................... 69
第七節 統計方法 ......................................... 71
第四章 實驗結果 ......................................... 72
第一節 柴胡疏肝散75%乙醇萃取物HPLC 成分分析圖 ........... 72
第二節 柴胡疏肝散75%乙醇萃取物(CSS)急性毒性試驗 ......... 76
第三節 柴胡疏肝散75%乙醇萃取物(CSS)對高膽固醇飲食誘導高血
脂大鼠之影響 ............................................ 77
第四節 柴胡疏肝散75%乙醇萃取物(CSS)對高血脂大鼠肝臟組織之
影響 .................................................... 93
第五節 動物肝臟 ........................................ 101
第五章 討論............................................. 112
第六章 結論............................................. 119
參考文獻 ............................................... 121

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