臺灣博碩士論文加值系統

本論文藉由超聲波震盪法將微米大小的MoS2粉末製備成MoS2量子點分散液，再加入金奈米粒子作為光學量測輔助並得知其光學特性。本論文選用之分散液溶劑為NMP，被認為具有與MoS2較接近的表面能量，使製備出之分散液具有良好的穩定性。再來我們對於MoS2分散液進行TEM的量測，來觀察MoS2量子點的大小與形貌。然而我們所觀測到的量子點尺寸約為3.349nm且分布均勻，並透過EDX檢測來驗證元素的存在，由於MoS2奈米粒子尺寸< 100 nm 時具有光致發光的特性，因此我們對於所製備之樣本使用325 nm 波長之He-Cd雷射進行激發並產生相應的光源，而在拉曼量測部分，藉由加入金奈米粒子增強訊號可以觀察到合成出的量子點具有特徵峰值可以驗證其材料特性，另外也會觀察到吸收光譜往短波長移動的現象。在成功合成量子點後，將其與蛋白質結合並量測與不同濃度下的蛋白質結合所產生的螢光強度，我們可以推斷出螢光效果最好的濃度進而應用到蛋白質螢光標記之生物領域。
關鍵字: 二硫化鉬、量子點、金奈米粒子、蛋白質、螢光

In this paper, micron-sized MoS2 powder was prepared into MoS2 quantum dots dispersion by ultrasonic vibration method, and then gold nanoparticles were added as optical measurement aid to know its optical properties. The dispersion solvent selected in this paper is NMP, which is considered to have a surface energy close to that of MoS2, so that the prepared dispersion has good stability. Next, we measured the TEM of the MoS2 dispersion to observe the size and morphology of the MoS2 quantum dots. However, the size of quantum dots were about 3.349 nm and evenly distributed then the presence of the elements was verified by EDX detection. When the size of MoS2 nanoparticles is < 100 nm, it has photoluminescence properties. Therefore, we used the He-Cd laser at 325 nm to excite and generate the corresponding light source. In the Raman measurement section, the signal is enhanced by adding Au nanoparticles so that the synthesized quantum dots can be observed to have characteristic peaks. This verifies its material properties. In addition, the phenomenon that the absorption peak shifts to a short wavelength is also observed. After successfully synthesizing quantum dots, combining them with proteins and measuring the fluorescence intensity produced by binding to proteins at different concentrations, we can infer the best concentration of fluorescence and apply it to the biological field of protein fluorescent labeling.

Keywords: MoS2, Quantum dots, Au nanoparticles, Protein, Fluorescent

目錄 VI
圖目錄 X
第一章 緒論 1
1-1 前言 1
1-2 二維材料MoS2介紹 2
1-3 MoS2量子點的特性與應用 3
1-4蛋白質檢測介紹 4
1-5研究動機 6
1-6章節相關規劃 7
第二章 理論 17
2-1 二硫化鉬簡介 17
2-1-1 二硫化鉬物理特性 17
2-1-2 二硫化鉬化學特性 18
2-2 超聲波震盪剝離機制 19
2-3 量子點尺寸效應發光原理 19
2-4 MoS2量子點加入金奈米粒子增加拉曼訊號 21
2-5蛋白質濃縮螢光量測 24
2-5-1排斥集中效應 24
2-5-2濃度極化之離子消耗現象 25
第三章 實驗樣品與實驗方法 35
3-1實驗材料及藥品規格 35
3-2實驗流程及步驟 35
3-2-1 MoS2研磨前置作業 36
3-2-2 MoS2真空乾燥作業 37
3-2-3 MoS2乾燥後重新分散至溶劑中 37
3-2-4探針式超聲波剝離MoS2粒子 37
3-2-5超聲波震盪後續處理 38
3-2-6重力離心分離MoS2粒子 38
3-2-7從NMP轉移到水相 38
3-2-8分散液製作量測樣本 39
3-2-9分散液與BSA進行混合 39
3-3實驗儀器及裝置 39
3-3-1探針式超聲波震盪儀 39
3-3-2離心機 40
3-3-3循環式烘箱系統 40
3-3-4超音波洗淨機 40
3-4 分析儀器 41
3-4-1拉曼光譜分析儀(Raman spectrometer) 41
3-4-2穿透式電子顯微鏡(TEM) 42
3-4-3能量色散X射線光譜儀(EDS) 43
3-4-4紫外光-可見光光譜儀 44
3-4-5光激螢光光譜(Photoluminescence，PL) 45
3-4-6 X光繞射分析(X-ray Diffraction, XRD) 45
第四章 實驗結果與討論 59
4-1 二硫化鉬量子點以及加入金奈米粒子之光 學與材料特性量測 59
4-1-1 TEM 量測 59
4-1-2 EDS 量測 59
4-1-3 UV–vis spectra 量測 60
4-1-4 PL spectra 量測 60
4-1-5 Raman 量測 61
4-1-6 XRD 量測 61
4-2二硫化鉬量子點加金奈米粒子與BSA混合之光學與材料特性量測 62
4-2-1 TEM 量測 62
4-2-2 EDS 量測 62
4-2-3 UV–vis spectra 量測 63
4-2-4 BSA Fluorescence Intensity 量測 63
第五章 結論與未來展望 77
參考文獻 79

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