臺灣博碩士論文加值系統

近年來，雉尾水雉在台灣的數量不斷下降，而且棲地也因人為活動而逐漸減少。其基因多樣性已成為一個在保育上的重要議題。本研究中以粒線體控制區作為基因標誌，以探索雉尾水雉在台灣、廣東、泰國三地之間的遺傳變異。總共從34隻個體中得到長度1182鹼基對之序列，只發現6個變異點以及7種不同的基因型，而且在這些基因型中彼此都很相似。在取樣的三個地區之間，其基因變異相當貧乏，而且存在有高度的基因交流。不過，這些結論尚有待更多的樣本數來加以驗證。

The number of Pheasant-tailed Jacana (Hydrophasianus chirurgus) in Taiwan has been reduced and habitats decreased because of human activities in many years. From a conservation perspective, the genetic diversity of this bird became an important subject. The control region in mitochondrial DNA was used as a gene marker to investigate the genetic variation amoung populations of Hydrophasianus chirurgus from Taiwan, Goungdong, and Thailand. An 1182-bp nucleotide sequence was amplified from 34 individuals. Six variable sites and seven haplotypes were found in the control region, and the observed haplotypes were similar in all samples. Low genetic divergence and high degree of gene flow were estimated among three populations. However, this conclusion need more samples of individuals to be proved.

Introduction……………………………………………………………...1
Methods and Materials…………………………………………………4
Results…………………………………………………………………..9
Discussion……………………………………………………………..13
Reference…………………………………………………………...…17

Figures
1. The records of Hydrophasianus chirurgus from 1977 to 1997……………...21
2. Sample localities…………………………………………………………………22
3. Entropy plot of the D-loop sequence…………………………………………..23
4. Distribution of the conserved boxes in D-loop………………………………..24
5. Phylogenetic relationships……………………………………………………...25
6. The TCS cladogram……………………………………………………………..26
7. The minimum spanning network……………………………………………….27

Table
1. The samples used in this study………………………………………………..28
2. The morphological measurements in different sex…………………………..29
3. The morphological measurements in the same sex…………………………30
4. The base composition and variance in D-loop……………………………….31
5. The variable sites in D-loop…………………………………………………….32
6. The pariwise distance…………………………………………………………..33
7. The result of AMOVA……………………………………………………………34
8. The result about population genetics………………………………………….35

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