臺灣博碩士論文加值系統

蝶豆花（學名：Clitoria ternatea）為豆科蝶豆屬的植物，具有抗氧化、抗菌、美白等功效，將植物根、葉、種子、花瓣進行品質檢測，結果顯示蝶豆花花瓣具有高純度，其總灰分含量2.87±0.49 g，及酸不溶灰分0.31±0.10 g，故實驗選用蝶豆花花瓣進行功能性評估，分析其抗氧化、美白、紫外光吸收、及成分含量。
本研究探討改變蝶豆花用量及溶劑比例，評估各種活性功效及化妝品潛力之應用，結果顯示蝶豆花萃取液皆具有紫外光吸收能力，其中萃取濃度3%之蝶豆花70%乙醇萃取液，吸收UVA的能力較強，吸光值在400nm為3.493abs，總花青素在40g蝶豆花含量最高 316 mg。
另外，不同溶劑及濃度之萃取液，DPPH和ABTS自由基，抗氧化及清除自由基能力有差異，其中萃取濃度3%浸泡1小時之CT E 5，清除能力較高為329.15±0.75mg/mL與281.89±2.59 mg/mL為最佳結果。同時將最佳抗氧化能力萃取液，探討蝶豆花萃取液體保存度，發現濃度3%萃取1小時之蝶豆花50%乙醇萃取液，萃取後，放置冰箱10小時的效果最佳289.89 mg/ml，其次為萃取後置於室溫25°C 10小時274.15mg/ml，而CT E 7抑制酪胺酸酶能力最佳46.79±2.65 mg/ml。

Butterfly bean flower (scientific name: Clitoria ternatea) is a plant of the genus Rhododendron, which have antioxidant, antibacterial and whitening effects. By quality testing of plant roots, leaves, seeds, and petals, The petal had the highest purity, and the total ash content was 2.87±0.49 g, and acid-insoluble ash content was 0.31±0.10 g. Therefore, the flower petals ware performed for functional evaluation, antioxidant analysis, whitening, UV absorption, and component content.
This study explored the application on changing the amount of butterfly flowers and the proportion of solvent, to evaluated the various active functions and cosmetic potentials. The results showed that the extracts of the butterfly bean flower had ultraviolet light absorption capacity, and the C.T.E 7 with a concentration of 3% had stronger UV absorbance than the other extractions.
The absorbances were 0.923 and 2.543 abs regtitively , on 400 and 280 nm,
Than the total anthocyanin content is 316mg in 40g petals.
In addition, the different solvents and concentrations of extracts. Resulted in different DPPH and ABTS free radicals, and different antioxidant and free radical scavenging ability, Among the extracts 3% soaked for 1 hour and the
butterfly bean 50% ethanol extract. had higher removal capacity which are
329.15 ± 0.75 mg/mL and 281.89±2.59 mg/mL, regtitively the optimal antioxidant capacity extract was also used to investigate the liquid preservation
capacities of the butterfly bean extracts. at was found that of 3% extraction of the butterfly bean with 50% ethanol extract for 1 hour, After extraction showed the best effect, By storing in the refrigerator for 10 hours 289.89 mg/ml.
Also, it’s best to be placed at room temperature 25 °C for 10 hours (274.15 mg / ml) after extraction. CT E 7 has the best ability to inhibit tyrosinase
(46.79±2.65 mg/ml.)

摘要 I
Abstract II
誌謝 III
目錄 IV
圖目錄 VII
表目錄 IX
縮寫表 X
第一章 緒論 11
1-1前言 11
1-2 研究目的與動機 13
第二章 文獻回顧 14
2-1 蝶豆花簡介 14
2-1-1蝶豆花 14
2-1-2蝶豆花之藥理作用 14
2-2皮膚老化 19
2-3何謂自由基 20
2-4自由基的防禦機制 22
2-5 氧化壓力防禦系統 23
2-6 黑色素介紹 25
第三章、材料與方法 27
3.1 實驗架構 27
3.2實驗材料 28
3.3 實驗材料製備與方法 31
3.3.1天然物萃取物 31
3.4 品質控管檢驗 32
3.4.1 灰分定量 32
3.4.2 酸不溶灰分定量 33
3.5抗氧化能力評估 34
3.5.1 DPPH自由基清除試驗 34
3.5.2 ABTS陽離子自由基清除試驗 36
3.6 紫外光光譜評估 38
3.7總花青素定量 40
3.9抑制體外酪胺酸酶 42
第四章、結果與討論 44
4.1品質控管檢測 44
4.1.1總灰分含量 錯誤! 尚未定義書籤。
4.1.2酸不溶含量 錯誤! 尚未定義書籤。
4.2 蝶豆花之萃取率 45
4.3有效成分鑑定之總花素定量 46
4.4紫外光吸收光譜 47
4.5 抗氧化能力試驗 50
4.5.1 DPPH．自由基清除試驗 50
4.5.2 ABTS+．陽離子自由基清除試驗 58
4.6 抑制體外酪胺酸酶 66
4.7 樣品保存能力評估 67
第五章、結論 68
第六章、參考文獻 69

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