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研究生:林俊良
研究生(外文):Jiun-liang Lin
論文名稱:鯽魚膽根的水萃物對大鼠肝星狀細胞之影響
論文名稱(外文):Effects of the Aqueous Extract of Pluchea indica Root on Hepatic Stellate Cells of Rat
指導教授:卓忠隆
指導教授(外文):Chung-Lung Cho
學位類別:碩士
校院名稱:國立中山大學
系所名稱:生物科學系研究所
學門:生命科學學門
學類:生物學類
論文種類:學術論文
論文出版年:2010
畢業學年度:98
語文別:中文
論文頁數:84
中文關鍵詞:肝纖維化肝星狀細胞細胞增生細胞移行
外文關鍵詞:liver fibrosisHSCscell proliferationcell migration
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肝纖維化為肝臟不斷受到不正常損傷刺激,反覆修補所造成細胞外基質不正常沉積所造成的結果,肝臟受損的過程中,肝星狀細胞受到細胞激素的刺激而由靜默態轉型成為活化態,造成肝星狀細胞大量的增生及細胞外基質的分泌,並因細胞外基質的生成以及降解的不平衡,導致過度的堆積以及異常的分佈,而形成肝纖維化。研究指出鯽魚膽具有抗發炎、抗潰瘍、抗氧化等功效,而本實驗室之前研究發現鯽魚膽根的水萃物有效抑制子宮頸癌細胞HeLa以及腦癌細胞GBM8401的細胞增生速率,更在鯽魚膽對硫代乙醯氨(TAA)誘發肝纖維化的小鼠中,具有降低肝臟發炎指標血清GPT的活性、肝星狀細胞活化指標α-平滑肌動蛋白(α-SMA)以及第一型膠原蛋白的表現。本研究主要目的為評估鯽魚膽根的水萃物是否能抑制肝星狀細胞活化、增生以及移行之能力。結果顯示,肝星狀細胞在處理0.5 mg/ml或1.0 mg/ml 鯽魚膽根的水萃物48小時過後,能有效降低肝星狀細胞活化指標蛋白α-SMA以及第一型膠原蛋白之表現。另外,由生長曲線、MTT、WST-1以及BrdU等分析之結果發現鯽魚膽根的水萃物有效抑制肝星狀細胞增生,並隨著濃度以及時間的增加而提高細胞增生抑制的情形。此外,由wound healing實驗分析及transwell實驗分析發現鯽魚膽根的水萃物能有效抑制肝星狀細胞之移行能力。綜合上述實驗的結果證明鯽魚膽根的水萃物具有抑制肝臟星狀細胞活化、增生、移行以及第一型膠原蛋白的表現之能力。
Liver fibrosis is a wound healing process in liver with chronic injury and is characterized by the excess production and accumulation of extracellular matrix (ECM) component. Liver injury of any etiology may lead to activation of hepatic stellate cells (HSCs), which are trans-differentiated from lipocyte-like cells to highly proliferative myofibroblast-like cells. Activation of HSCs is considered a crucial event that promotes increased ECM production and consequently hepatic fibrosis. Liver fibros is resulted from a net increased synthesis and decreased degradation of ECM proteins. Pluchea indica (Less) has been reported to have antipyretic, anti-ulcer, anti-inflammatory, anti-oxidant, diuretic and anti-amoebic activities. Our previous studies showed that the aqueous extract of roots from P. indica (PIRAE) showed that it can suppress the growth and migration of HeLa and GBM8401 cancer cell lines, and also significantly reduce serum glutamate pyruvate transaminase (GPT), alpha-smooth muscle actin (α-SMA) and collagen type I expression in animal model of liver fibrosis induced by thioacetamide (TAA). In this study, we plan to investigate the effects of PIRAE on activation, proliferation and migration of rat culture activated HSCs.
The results indicated that protein expression of α-SMA and collagen type I of HSCs was decreased followed by treatment of either 0.5 or 1.0 mg/ml PIRAE for 48 hours. In addition, the effects of PIRAE on proliferation in culture activated HSCs were assessed by analyses of cell growth curve, MTT, WST-1 and BrdU, respectively. The results showed that PIRAE inhibited HSCs proliferation in a dose- and time-dependent manner. Moreover, wound healing assay and transwell assay showed that PIRAE prevented migration in activated HSCs. In conclusion, PIRAE may suppresse culture activated HSCs proliferation, migration, and activation of culture activated HSCs, as well as accumulation of collagen type I.
中文摘要 iii
Abstract v
壹、緒論 1
一、前言 1
二、肝臟細胞 1
三、肝星狀細胞簡介 2
1. 靜默態肝星狀細胞與活化態肝星狀細胞 2
2. 肝星狀細胞體外活化以及限制 3
3. 肝星狀細胞的可逆性 5
4. 肝星狀細胞的異質性以及物種特異性 5
四、肝纖維化與肝星狀細胞 6
五、草藥在肝纖維化的研究 7
1. 草藥的優勢 7
2. 水飛薊 8
3.日本草藥(TJ-series) 9
六、鯽魚膽 11
1. 鯽魚膽形態與分布 11
2. 鯽魚膽之研究與應用 12
貳、實驗目的 14
參、材料與方法 15
一、鯽魚膽根的水萃物之製備 15
二、肝星狀細胞分離 15
三、免疫細胞化學染色 17
四、免疫螢光染色 18
五、細胞增生分析 20
1. 生長曲線分析 20
2. MTT assay 20
3. WST-1 assay 21
4. BrdU assay 22
六、西方墨點法 23
1. 蛋白質萃取 24
2. 蛋白質定量 24
3. 蛋白質電泳 24
4. 轉漬以及blocking 25
5. 抗體接合以及呈色反應 25
七、細胞移行分析 26
1. Wound healing assay 26
2. Transwell assay 27
肆、結果 28
一、肝星狀細胞分離培養及型態之變化 28
二、肝星狀細胞確認 28
三、鯽魚膽對肝星狀細胞型態之影響 29
四、鯽魚膽對肝星狀細胞增生之影響 30
1. 生長曲線 30
2. MTT assay 31
3. WST-1 assay 32
4. BrdU assay 32
五、鯽魚膽對肝星狀細胞α-SMA表現之影響 33
六、鯽魚膽對肝星狀細胞collagen type I表現之影響 34
七、鯽魚膽對活化肝星狀細胞移行的影響 34
1. Wound healing 35
2. Transwell assay 35
伍、討論 37
陸、參考文獻 42
附錄 66

1.Friedman SL. 2000. Molecular regulation of hepatic fibrosis, an integrated cellular response to tissue injury. J Biol Chem 275: 2247-50

2.Friedman SL. 2003. Liver fibrosis -- from bench to bedside. J Hepatol 38 Suppl 1: S38-53

3.Gines P, Cardenas A, Arroyo V, Rodes J. 2004. Management of cirrhosis and ascites. N Engl J Med 350: 1646-54

4.Iredale JP. 2003. Cirrhosis: new research provides a basis for rational and targeted treatments. BMJ 327: 143-7

5.Schuppan D, Ruehl M, Somasundaram R, Hahn EG. 2001. Matrix as a modulator of hepatic fibrogenesis. Semin Liver Dis 21: 351-72

6.Friedman SL. 1993. Seminars in medicine of the Beth Israel Hospital, Boston. The cellular basis of hepatic fibrosis. Mechanisms and treatment strategies. N Engl J Med 328: 1828-35

7.Gressner AM, Lotfi S, Gressner G, Haltner E, Kropf J. 1993. Synergism between hepatocytes and Kupffer cells in the activation of fat storing cells (perisinusoidal lipocytes). J Hepatol 19: 117-32

8.Shi Z, Wakil AE, Rockey DC. 1997. Strain-specific differences in mouse hepatic wound healing are mediated by divergent T helper cytokine responses. Proc Natl Acad Sci U S A 94: 10663-8

9.Hemmann S, Graf J, Roderfeld M, Roeb E. 2007. Expression of MMPs and TIMPs in liver fibrosis - a systematic review with special emphasis on anti-fibrotic strategies. J Hepatol 46: 955-75

10.Friedman SL, Roll FJ, Boyles J, Bissell DM. 1985. Hepatic lipocytes: the principal collagen-producing cells of normal rat liver. Proc Natl Acad Sci U S A 82: 8681-5
11.Geerts A. 2001. History, heterogeneity, developmental biology, and functions of quiescent hepatic stellate cells. Semin Liver Dis 21: 311-35

12.PL W. 1995. Gray’s Anatomy:the anatomical basis of Medicine and surgery.

13.Kmiec Z. 2001. Cooperation of liver cells in health and disease. Adv Anat Embryol Cell Biol 161: III-XIII, 1-151

14.Hautekeete ML, Geerts A. 1997. The hepatic stellate (Ito) cell: its role in human liver disease. Virchows Arch 430: 195-207

15.Tanikawa K. 1999. Liver Diseases And Hepatic Sinusoidal Cells: Springer-verlag 356 pp.

16.Senoo H. 2004. Structure and function of hepatic stellate cells. Med Electron Microsc 37: 3-15

17.Marra F. 1999. Hepatic stellate cells and the regulation of liver inflammation. J Hepatol 31: 1120-30

18.Burt AD. 1999. Pathobiology of hepatic stellate cells. J Gastroenterol 34: 299-304

19.Friedman SL. 1996. Hepatic stellate cells. Prog Liver Dis 14: 101-30

20.Kurosaka K, Watanabe N, Kobayashi Y. 2001. Production of proinflammatory cytokines by resident tissue macrophages after phagocytosis of apoptotic cells. Cell Immunol 211: 1-7

21.Friedman SL, Rockey DC, McGuire RF, Maher JJ, Boyles JK, Yamasaki G. 1992. Isolated hepatic lipocytes and Kupffer cells from normal human liver: morphological and functional characteristics in primary culture. Hepatology 15: 234-43

22.Iredale JP, Benyon RC, Arthur MJ, Ferris WF, Alcolado R, Winwood PJ, Clark N, Murphy G. 1996. Tissue inhibitor of metalloproteinase-1 messenger RNA expression is enhanced relative to interstitial collagenase messenger RNA in experimental liver injury and fibrosis. Hepatology 24: 176-84

23.Winwood PJ, Schuppan D, Iredale JP, Kawser CA, Docherty AJ, Arthur MJ. 1995. Kupffer cell-derived 95-kd type IV collagenase/gelatinase B: characterization and expression in cultured cells. Hepatology 22: 304-15

24.Arroyo V. 2002. Pathophysiology, diagnosis and treatment of ascites in cirrhosis. Ann Hepatol 1: 72-9

25.Kristensen DB, Kawada N, Imamura K, Miyamoto Y, Tateno C, Seki S, Kuroki T, Yoshizato K. 2000. Proteome analysis of rat hepatic stellate cells. Hepatology 32: 268-77

26.Sato M, Suzuki S, Senoo H. 2003. Hepatic stellate cells: unique characteristics in cell biology and phenotype. Cell Struct Funct 28: 105-12

27.Friedman SL, Yamasaki G, Wong L. 1994. Modulation of transforming growth factor beta receptors of rat lipocytes during the hepatic wound healing response. Enhanced binding and reduced gene expression accompany cellular activation in culture and in vivo. J Biol Chem 269: 10551-8

28.Friedman SL, Roll FJ, Boyles J, Arenson DM, Bissell DM. 1989. Maintenance of differentiated phenotype of cultured rat hepatic lipocytes by basement membrane matrix. J Biol Chem 264: 10756-62

29.Sohara N, Znoyko I, Levy MT, Trojanowska M, Reuben A. 2002. Reversal of activation of human myofibroblast-like cells by culture on a basement membrane-like substrate. J Hepatol 37: 214-21


30.Iredale JP. 2001. Hepatic stellate cell behavior during resolution of liver injury. Semin Liver Dis 21: 427-36

31.Ramm GA, Britton RS, O''Neill R, Blaner WS, Bacon BR. 1995. Vitamin A-poor lipocytes: a novel desmin-negative lipocyte subpopulation, which can be activated to myofibroblasts. Am J Physiol 269: G532-41

32.Ballardini G, Groff P, Badiali de Giorgi L, Schuppan D, Bianchi FB. 1994. Ito cell heterogeneity: desmin-negative Ito cells in normal rat liver. Hepatology 19: 440-6

33.Schmitt-Graff A, Desmouliere A, Gabbiani G. 1994. Heterogeneity of myofibroblast phenotypic features: an example of fibroblastic cell plasticity. Virchows Arch 425: 3-24

34.Lai CL, Shouval D, Lok AS, Chang TT, Cheinquer H, Goodman Z, DeHertogh D, Wilber R, Zink RC, Cross A, Colonno R, Fernandes L. 2006. Entecavir versus lamivudine for patients with HBeAg-negative chronic hepatitis B. N Engl J Med 354: 1011-20

35.Chang TT, Gish RG, de Man R, Gadano A, Sollano J, Chao YC, Lok AS, Han KH, Goodman Z, Zhu J, Cross A, DeHertogh D, Wilber R, Colonno R, Apelian D. 2006. A comparison of entecavir and lamivudine for HBeAg-positive chronic hepatitis B. N Engl J Med 354: 1001-10

36.Arora G, Keeffe EB. 2007. Chronic hepatitis B with advanced fibrosis or cirrhosis: impact of antiviral therapy. Rev Gastroenterol Disord 7: 63-73

37.Kurashige N, Ohkawa K, Hiramatsu N, Yakushijin T, Mochizuki K, Oze T, Kiso S, Kanto T, Takehara T, Kasahara A, Doi Y, Yamada A, Fukuda K, Oshita M, Mita E, Fukui H, Nagase T, Yoshihara H, Imai Y, Kato M, Kashihara T, Hayashi N. 2009. Lamivudine-to-entecavir switching treatment in type B chronic hepatitis patients without evidence of lamivudine resistance. J Gastroenterol 44: 864-70
38.Cho SW, Koh KH, Cheong JY, Lee MH, Hong SP, Yoo WD, Kim SO. 2010. Low efficacy of entecavir therapy in adefovir-refractory hepatitis B patients with prior lamivudine resistance. J Viral Hepat 17: 171-7

39.Jia JD, Bauer M, Cho JJ, Ruehl M, Milani S, Boigk G, Riecken EO, Schuppan D. 2001. Antifibrotic effect of silymarin in rat secondary biliary fibrosis is mediated by downregulation of procollagen alpha1(I) and TIMP-1. J Hepatol 35: 392-8

40.Wagner H SO, Seitz M, Abraham D. 1976. Sonnenbichler J.Silydianin und Silychristin, zwei isomere Silymarine aus Silybum marianum (Mariendistel). Z Naturforsch 31: 876-84

41.Dehmlow C, Erhard J, de Groot H. 1996. Inhibition of Kupffer cell functions as an explanation for the hepatoprotective properties of silibinin. Hepatology 23: 749-54

42.Mourelle M, Muriel P, Favari L, Franco T. 1989. Prevention of CCL4-induced liver cirrhosis by silymarin. Fundam Clin Pharmacol 3: 183-91

43.Jia JD BG, Bauer M, Ruehl M, Strefeld T, Riecken EO. 1998. Silymarin downregulates TIMP-1 and collagen I mRNA in rats with secondary biliary cirrhosis. Hepatology 28(suppl.): 546A

44.Buzzelli G, Moscarella S, Giusti A, Duchini A, Marena C, Lampertico M. 1993. A pilot study on the liver protective effect of silybin-phosphatidylcholine complex (IdB1016) in chronic active hepatitis. Int J Clin Pharmacol Ther Toxicol 31: 456-60

45.Shimizu I, Ma YR, Mizobuchi Y, Liu F, Miura T, Nakai Y, Yasuda M, Shiba M, Horie T, Amagaya S, Kawada N, Hori H, Ito S. 1999. Effects of Sho-saiko-to, a Japanese herbal medicine, on hepatic fibrosis in rats. Hepatology 29: 149-60

46.Kyo R, Nakahata N, Sakakibara I, Kubo M, Ohizumi Y. 1998. Effects of Sho-saiko-to, San''o-shashin-to and Scutellariae Radix on intracellular Ca2+ mobilization in C6 rat glioma cells. Biol Pharm Bull 21: 1067-71

47.Sakaida I, Hironaka K, Kimura T, Terai S, Yamasaki T, Okita K. 2004. Herbal medicine Sho-saiko-to (TJ-9) increases expression matrix metalloproteinases (MMPs) with reduced expression of tissue inhibitor of metalloproteinases (TIMPs) in rat stellate cell. Life Sci 74: 2251-63

48.Chen MH, Chen JC, Tsai CC, Wang WC, Chang DC, Lin CC, Hsieh HY. 2004. Sho-saiko-to prevents liver fibrosis induced by bile duct ligation in rats. Am J Chin Med 32: 195-207

49.Wang BJ, Liu CT, Tseng CY, Wu CP, Yu ZR. 2004. Hepatoprotective and antioxidant effects of Bupleurum kaoi Liu (Chao et Chuang) extract and its fractions fractionated using supercritical CO(2) on CCl(4)-induced liver damage. Food Chem Toxicol 42: 609-17

50.Kusunose M, Qiu B, Cui T, Hamada A, Yoshioka S, Ono M, Miyamura M, Kyotani S, Nishioka Y. 2002. Effect of Sho-saiko-to extract on hepatic inflammation and fibrosis in dimethylnitrosamine induced liver injury rats. Biol Pharm Bull 25: 1417-21

51.Chen MH, Chen JC, Tsai CC, Wang WC, Chang DC, Tu DG, Hsieh HY. 2005. The role of TGF-beta 1 and cytokines in the modulation of liver fibrosis by Sho-saiko-to in rat''s bile duct ligated model. J Ethnopharmacol 97: 7-13

52.Oka H, Yamamoto S, Kuroki T, Harihara S, Marumo T, Kim SR, Monna T, Kobayashi K, Tango T. 1995. Prospective study of chemoprevention of hepatocellular carcinoma with Sho-saiko-to (TJ-9). Cancer 76: 743-9

53.Gibo Y NY, Takahashi N, Inada H, Nakagawa M, Usuda S, Nakano Y. 1994. Clinical study of Sho-saiko-to therapy to the Japanese patients with chronic hepatitis type C (CH-C). Prog Med 14: 217-9
54.Tajiri H, Kozaiwa K, Ozaki Y, Miki K, Shimuzu K, Okada S. 1991. Effect of sho-saiko-to(xiao-chai-hu-tang) on HBeAg clearance in children with chronic hepatitis B virus infection and with sustained liver disease. Am J Chin Med 19: 121-9

55.Inao M, Mochida S, Matsui A, Eguchi Y, Yulutuz Y, Wang Y, Naiki K, Kakinuma T, Fujimori K, Nagoshi S, Fujiwara K. 2004. Japanese herbal medicine Inchin-ko-to as a therapeutic drug for liver fibrosis. J Hepatol 41: 584-91

56.Imanishi Y, Maeda N, Otogawa K, Seki S, Matsui H, Kawada N, Arakawa T. 2004. Herb medicine Inchin-ko-to (TJ-135) regulates PDGF-BB-dependent signaling pathways of hepatic stellate cells in primary culture and attenuates development of liver fibrosis induced by thioacetamide administration in rats. J Hepatol 41: 242-50

57.Sakaida I, Tsuchiya M, Kawaguchi K, Kimura T, Terai S, Okita K. 2003. Herbal medicine Inchin-ko-to (TJ-135) prevents liver fibrosis and enzyme-altered lesions in rat liver cirrhosis induced by a choline-deficient L-amino acid-defined diet. J Hepatol 38: 762-9

58.Yamamoto M, Ogawa K, Morita M, Fukuda K, Komatsu Y. 1996. The herbal medicine Inchin-ko-to inhibits liver cell apoptosis induced by transforming growth factor beta 1. Hepatology 23: 552-9

59.Ikeda H, Nagashima K, Yanase M, Tomiya T, Arai M, Inoue Y, Tejima K, Nishikawa T, Watanabe N, Kitamura K, Isono T, Yahagi N, Noiri E, Inao M, Mochida S, Kume Y, Yatomi Y, Nakahara K, Omata M, Fujiwara K. 2006. The herbal medicine inchin-ko-to (TJ-135) induces apoptosis in cultured rat hepatic stellate cells. Life Sci 78: 2226-33

60.Editorial Committee of the Flora of Taiwan FoT. 2003. Flora of Taiwan.


61.Peng CI CC, Leu WP, Yen HF. 1998. Pluchea Cass. (Asteraceae: Inuleae) in Taiwan. Bot. Bull. Acad. Sin.: 287-97

62.陳虹妃. 2003. 氯化鈉對鯽魚膽切離葉片之影響. 國立高雄師範大學生物科學研究所碩士論文

63.陳玉樹. 2002. 鹽與乾旱逆境對屋山頂火山地區植物分佈之影響. 國立高雄師範大學生物科學研究所碩士論文

64.K.R. Kirtikar BDB. 1999. Indian Medicinal Plants: Dehradun and Delhi: International Book Distributors

65.Pramanik KC BP, Biswas R, Bandyopadhyay D, Mishra M,Chatterjee TK. 2006. Hypoglycemic and antihyperglycemic activity of leaf extract of Pluchea indica Less. Orient Pharm Exp Med 6: 232-6

66.Pramanik KC BR, Mitra A, Bandyopadhyay D, Mishra M, Chatterjee TK. 2007. Tissue Culture of the plant Pluchea indica (L.) Less. and Evaluation of Diuretic Potential of its Leaves. Orient Pharm Exp Med 7: 197-204

67.Sen T, Nag Chaudhuri AK. 1991. Antiinflammatory evaluation of a Pluchea indica root extract. J Ethnopharmacol 33: 135-41

68.Pal S NCA. 1989. Studies on the effect of Pluchea indica Less. root extract on gastroduodenal ulcer models in rats and guinea pig. Phytother Res 3: 156-8

69.Ria Biswas AD, Anupama Mitra, Subodh K. Roy , Pradip K. Dutta, Basudeb Achari, Sujata Ghosh Dastidar, Tapan K. Chatterjee*,. 2005. Isolation, purification and characterization of four pure compounds from the root extract of Pluchea indica (L.) Less. and the potentiality of the root extract and the pure compounds for antimicrobial activity. European Bulletin of Drug Research 13: 63–70

70.Sen T, Dhara AK, Bhattacharjee S, Pal S, Nag Chaudhuri AK. 2002. Antioxidant activity of the methanol fraction of Pluchea indica root extract. Phytother Res 16: 331-5

71.Thongpraditchote S, Matsumoto K, Temsiririrkkul R, Tohda M, Murakami Y, Watanabe H. 1996. Neuropharmacological actions of Pluchea indica Less root extract in socially isolated mice. Biol Pharm Bull 19: 379-83

72.Biswas R, Dutta PK, Achari B, Bandyopadhyay D, Mishra M, Pramanik KC, Chatterjee TK. 2007. Isolation of pure compound R/J/3 from Pluchea indica (L.) Less. and its anti-amoebic activities against Entamoeba histolytica. Phytomedicine 14: 534-7

73.Gomes A, Saha A, Chatterjee I, Chakravarty AK. 2007. Viper and cobra venom neutralization by beta-sitosterol and stigmasterol isolated from the root extract of Pluchea indica Less. (Asteraceae). Phytomedicine 14: 637-43

74.曹淑娟. 2007. 探討鯽魚膽根與葉之水萃物對癌細胞的影響. 國立中山大學生物科學系碩士在職專班碩士論文

75.吳麗娟. 2009. 鯽魚膽水萃物對硫代乙醯胺誘發小鼠肝纖維化的保護作用. 國立中山大學生物科學系碩士在職專班碩士論文

76.Li XK, Motwani M, Tong W, Bornmann W, Schwartz GK. 2000. Huanglian, A chinese herbal extract, inhibits cell growth by suppressing the expression of cyclin B1 and inhibiting CDC2 kinase activity in human cancer cells. Mol Pharmacol 58: 1287-93


77.Lee KS, Buck M, Houglum K, Chojkier M. 1995. Activation of hepatic stellate cells by TGF alpha and collagen type I is mediated by oxidative stress through c-myb expression. J Clin Invest 96: 2461-8

78.Bataller R, Brenner DA. 2001. Hepatic stellate cells as a target for the treatment of liver fibrosis. Semin Liver Dis 21: 437-51

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2. 21.林繼生:〈語文表達能力測驗──大學入學考試的新神主牌?〉,《國文天地》16卷8期,2001年1月。
3. 47.楊鴻銘:〈作文教室(13)文章仿寫的作法──以「不亦快哉」為例〉,《國文天地》12卷10期,1997年3月。
4. 16.李靜雯:〈論「限制式寫作」題組在國中寫作教學中的運用──鎖定主旨置於篇末與篇外的能力〉,《國文天地》22卷2期,2006年7月。
5. 29.陳滿銘:〈談命題作文的分項指引〉,《國文天地》19卷4期,2003年9月。
6. 9.仇小屏:〈「限制式寫作」題型與能力〉,《國文天地》21卷3期,2005年8月。
7. 43.楊鴻銘:〈作文教室(6)作文多向訓練的方法──以「變」為例〉,《國文天地》11卷11期,1996年4月。
8. 14.仇小屏:〈「摹寫」格名稱及其內涵的再商榷〉,《國文天地》23卷11期,2008年4月。
9. 37.黃心怡:〈續寫題型的理論初探〉,《國文天地》23卷8期,2008年1月。
10. 25.陳正治:〈作文教學法介紹與探討〉,《國教新知》55卷1期,2008年3月。
11. 56.楊鴻銘:〈95年大學學測國文科作文題解析〉,《中國語文》98卷3期,2006年3月。
12. 10.仇小屏:〈歷屆升大學考試「新型作文」考題之分析與檢討〉,《國文天地》21卷4期,2005年9月。
13. 44.楊鴻銘:〈作文多體訓練的方法──以今年大學聯考國文作文「自由與自律」為例〉,《中國語文》79卷2期,1996年8月。
14. 20.林素珍:〈由新型作文談閱讀與寫作的關係〉,《國文天地》25卷5期,2009年10月。
15. 41.楊鴻銘:〈作文教室(4)提問作文的方法──以「是與非」為例〉,《國文天地》11卷8期,1996年1月。