臺灣博碩士論文加值系統

本研究藉由結合ferrocene硫醇分子之操控與智慧結構(smart structure)之概念，建構一套適用於生醫系統之微型元件開發方案。雖然智慧型材料具有製備簡便與提升元件效能等特色，然則其操縱原理多會造成生物分子之破壞而不適於生醫感測元件之開發。考量至此，吾人利用ferrocene分子之氧化活性，藉由電化學操控方式使之呈現雙性分子(amphiphlic)之特色，以達到操控元件效能之目標。對ferrocene分子之自我組裝層性能之探究上發現，利用短碳鏈分子之摻雜效應可擾亂ferrocene硫醇分子之排列，進而提升自我組裝層性質之變化範圍。而將ferrocenyl SAM結合於微流元件時，亦驗證藉由氧化ferrocenyl SAM方式，可調變微流道介面親水性，從而完成將流道內液體匯流比例控制於50%至70%之範疇。
於光波導元件之開發上，研究中先針對波導結構設計做探討。並藉由與硝酸纖維薄膜之整合，開發完成一具備流體輸送功能之簡易式之分析套組。另外，將ferrocenyl SAM製備於波導感測元件時，亦驗證ferrocenyl SAM之氧化可造成SPR共振波長偏移 2 nm，若將SAM摻雜C4分子時，其共振波長之偏移更可到達6 nm。當進一步利用ferrocenyl SAM之氧化對SPR訊號之調變效果結合至多感測區之波導感測元件時，亦可發現藉由ferrocenyl SAM之氧化可對個別SPR感測區之訊號稍為區隔。本研究開發完成一套借電活性分子達成微型元件開發之方案，未來將設法提升流體操縱與SPR訊號調變幅度等效能，以期足能應用至可攜式生醫元件或光電元件等商業應用，以裨益社會。

In this study, a microdevice development strategy, for which integrate the manipulation of ferrocenyl alkanethiols characteristics and idea of smart structures was investigated. The smart devices posed advantages of easy preparation and improve the device performance. However the stimuli principles of these materials might damage biomolecules and not suitable for biomedical devices development. In this study the ferrocenyl alkanethiols, for which the amphilphlic characteristics could be manipulated by electrochemical methods, was utilized for the device control. From the characterization of ferrocenyl alkanethiols, it was found doping of molecules with shorter chain length would disturbe the SAM organization, which provided more variant range. For a microfluidic device for which integrated with ferrocenyl SAM, it was proposed with interfacial hydrophilicity could be altered by oxidation of ferrocenyl SAM, which caused the fluidic infusion ratio of 50%~ 70% in converge channel.
In the development of optical waveguide device, the influence of structure design in waveguide was first evaluated, after that a point-of-care kit which integrated with waveguide sensor with nitrocellulose strip for fluidic transportation was proposed. In addition, as the ferrocenyl SAM was integrated with waveguide SPR sensor, it was demonstrated that oxidation of ferrocenyl SAM would cause 2 nm shift in SPR resonance wavelength, if the SAM was doped with C4, shift magnitude of 6 nm can be achieved moreover. As the ferrocenyl SAM was integrated with SPR waveguide sensor with serial aligned multi- sensing areas. It was found the SPR signal on each sensing area might be slightly distinguished by oxidation of ferrocenyl SAM. In this study a microdevices development strategy which based on redox- active smart materials was proposed. The device performances such as fluidic manipulation efficiency and SPR signal modulation magnitude would then be improved in the future. The author wishes the research results presented can be beneficial for the development of medical and optoelectronic devices in the future.

致謝 I
中文摘要 II
Abstract III
Contents V
Graphics index VII
Tables index IX
Chapter 1. Background 1
1.1 Introduction of microdevices in biomedical applications 1
1.2 Applications of smart materials in micro/ nano sciences 3
1.2.1 SMA based Neuron recording electrodes 3
1.2.2 Interfacial hydrophilicity treatment 4
1.2.3 Applications of smart materials in microfluidic devices or surface treatment 8
1.3 Motivation 9
Chapter 2. Characterization of ferrocenyl SAM 13
2.1 Preparation of self assembled monolayer on electrodes 14
2.2 Electrochemical properties of hybrid SAMs 15
2.3 Interfacial hydrophilicity analysis of hybrid SAMs 18
2.4 Influence of chain lengths to hybrid SAMs characteristics 20
2.5 Mechanisms of alternate interfacial characteristics by redox of ferrocenyl SAM 22
2.5.1 Contact angle analysis of various SAMs following redox reaction 22
2.5.2 FTIR analysis of ferrocenyl SAMs 26
2.6 Short summary 29
Chapter 3. Fluidic regulation by ferrocenyl SAM 32
3.1 Microchip fabrication 32
3.2 System setup 34
3.2.1 Immobilization of ferrocenyl SAM 34
3.2.2 Measurement system 34
3.3 Evaluation of fluidic transport in microchannel 36
Chapter 4. Application of ferrocenyl SAM to SPR signal modulation on waveguide sensor 39
4.1 Principles of surface Plasmon resonance 39
4.2 Experimental setup 40
4.2.1 Fabrication of waveguide chip 40
4.2.2 System setup 42
4.3 Influence of waveguide structure to spectral characteristics 43
4.3.1 Design of curvature structure 43
4.3.2 Relationship between sensing area length and signal response 45
4.4 Simplified sample transportation via nitrocellulose strip 46
4.5 Alternation of SPR characteristics with ferrocenyl SAM 48
4.5.1 Introduction 48
4.5.2 Preparation of self-assembled monolayer 49
4.5.3 Measurement of SPR signal on waveguide chip followed by oxidation of SAM 49
4.5.4 Electrochemical characteristics of ferrocenyl SAM 50
4.5.5 Effect of ferrocenyl SAM oxidation state on SPR signal 51
4.5.6 Simulations of the refractive indices of SAM 55
4.5.7 Short Summary 57
4.6 Development of biosensor based on ferrocenyl SAM modulated waveguide chip 57
4.6.1 Concept of ferrocenyl SAM modulated multi- sensing area waveguide sensor 58
4.6.2 Immunoassay on the ferrocenyl SAM modulated multi- sensing area waveguide SPR sensor 59
4.6.3 Summary 60
Chapter 5. Conclusion and future work 62
5.1 Discussion 62
5.2 Future work for microfluidic devices development 62
5.3 Future work for ferrocenyl modulated waveguide sensor 63
Chapter 6. References 64

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