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研究生:方郁惠
研究生(外文):Yu-Hui Fang
論文名稱:辨識幹細胞表面標誌充分必要條件之研究
論文名稱(外文):Investigation of Cell Surface Markers for Necessary and Sufficient Identification of Stem Cells
指導教授:王清正
指導教授(外文):Ching-Cheng Wang
學位類別:碩士
校院名稱:國立成功大學
系所名稱:製造工程研究所碩博士班
學門:工程學門
學類:機械工程學類
論文種類:學術論文
論文出版年:2004
畢業學年度:92
語文別:英文
論文頁數:25
中文關鍵詞:再生醫學幹細胞細胞表面標誌充分必要條件
外文關鍵詞:regenerative medicinecell surface markersnecessary and sufficient identificationstem cell
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  對人類的健康來說,再生醫學被期望能帶來革命性的貢獻。幹細胞的鑑定開啟了再生醫學的大門,但錯誤的鑑定方式對人類來說可能存在著極大的風險。到目前為止,細胞表面標誌被認為是鑑定體內幹細胞的最佳方法,此研究並可能提高幹細胞萃取的效率。在分離幹細胞的過程中,我們考量細胞表面標誌的充分必要條件,也就是對於一特定幹細胞來說,有別於其他細胞的細胞表面標誌的最小集合。並且假定針對所有可獲取的幹細胞,其所有細胞表面標誌的表現結果都正確無誤。之後我們可以藉由使用這些正確無誤的資訊,針對一特定的幹細胞指派一個或一群細胞表面標誌集合來進行更進一步的實驗。許多表現在幹細胞上的細胞表面標誌已經被檢驗出來,其結果也被公佈。但這些努力被應用在一特定細胞的萃取時,是真的有效嗎?既然我們還沒有能力去假設這些被檢驗出來的細胞表面標誌均是正確無誤的,以上這個問題也許可由我們選擇的特定細胞而找到答案。
  在本論文中,我們對每一人體幹細胞做有系統地蒐集所有曾用來鑑別該細胞的表面標誌。另外,我們對於每一個細胞表面標誌也有系統地表列,曾以該標誌鑑定的所有幹細胞。藉此,我們可指派鑑定特定細胞式細胞群的充分必要條件。
  Regenerative medicine is expected to have revolutionary contribution to human health, where identification of stem cells constitutes one of the initial efforts and misidentification shall introduce catastrophic risks to life. Today, cell surface marker has been considered the best method for identifying stem cells in vivo that might help in making the extracting of stem cells more efficient. Consider the necessary and sufficient set of cell surface markers for stem cell isolation, i.e., the minimal set of markers that uniquely separates the targeted stem cell from others. Imagine that we have error free results of all the markers tested on all the stem cells available. Then, we can assign necessary and sufficient set(s) of cell surface markers for a particular stem cell using that error free information. Numerous markers have been tested on numerous stem cells, and results have been reported. Can achievements of those efforts be applied to assist in assigning the necessary and sufficient set of cell surface markers for a targeted stem cell? Since we do not have the luxury of making available to use the imaginary error free results of all the markers tested on al the stem cells, the answer of the above question might well depend on the targeted stem cell.
  In this thesis we systematically organize, for every human stem cell, all the cell surface markers that have been tested on a particular stem cell. In addition, we systematically organize, for every cell surface marker, all the human stem cells that have been tested with a particular cell surface marker. Thereby, we achieve the capability to assign the necessary and sufficient set of markers for a minimal set of stem cells. In case that the minimal set of stem cells contains only the targeted stem cell, the necessary and sufficient set of cell surface markers for that targeted stem cell is achieved.
MANDARIN ABSTRACT Ⅰ
ENGLISH ABSTRACT Ⅱ
MANDARIN ACKNOWLEDGEMENTS Ⅲ
TABLE OF CONTENTS Ⅳ
LIST OF FIGURES Ⅴ
LIST OF TABLES Ⅴ

CHAPTER 1 INTRODUCTION 1

CHAPTER 2 STEM CELLS 2
2.1 Embryonic Stem Cells 4
2.2 Adult Stem Cell 5
2.3 Embryonic Germ Cells 7
CHAPTER 3 CELL SURFACE MARKERS 8

CHAPTER 4 ACHIEVEMENTS IN HUMAN CELL MARKERS 14
4.1 Differentiation Diagrams 14
4.2 Marker Table 16
4.3 Marker Database 17

CHAPTER 5 CONCLUSION AND DISSCUSSION 21

REFERENCES 24
BIOGRAPHY 26
[1]Amit, M., Carpenter, M.K., Inokuma, M.S., Chiu, C.P., Harris, C.P., Waknitz, M.A., Itskovitz-Eldor, J., and Thomson, J.A., “Clonally derived human embryonic stem cell lines maintain pluripotency and proliferative potential for prolonged periods of culture,“ Dev. Biol., 2000; 227: 271-278.
[2]Itskovitz-Eldor, J., Schuldiner, M., Karsenti, D., Eden, A., Yanuka, O., Amit, M., Soreq, H., and Benvenisty, N., “Differentiation of human embryonic stem cells into embryoid bodies comprising the three embryonic germ layers,” Mol. Med., 2000; 6: 88-95.
[3]Jackson, K., Majka SM, Wang H, Pocius J, Hartley CJ, Majesky MW, Entman ML, Michael LH, Hirschi KK, and Goodell MA, “Regeneration of ischemic cardiac muscle and vascular endothelium by adult stem cells,” J. Clin. Invest., 2001; 107: 1-8.
[4]James A. Thomson, Joseph Itskovitz-Eldor, Sander S. Shapiro, Michelle A. Waknitz, Jennifer J. Swiergiel, Vivienne S. Marshall, and Jeffrey M. Jones, “Embryonic Stem Cell Lines Derived from Human Blastocysts”, Science, Nov 1998; 282: 1145 - 1147.
[5]Khosla, S., Dean, W., Brown, D., Reik, W., and Feil, R., “Culture of preimplantation mouse embryos affects fetal development and the expression of imprinted genes,” Biol. Reprod., 2001; 64, 918-926.
[6]MF Pera, W Bennett, and DP Cerretti, “Expression of CD30 and CD30 ligand in cultured cell lines from human germ-cell tumors,” Lab Invest, Apr 1997; 76(4): 497-504.
[7]Reubinoff, B.E., Pera, M.F., Fong, C.Y., Trounson, A., and Bongso, A., “Embryonic stem cell lines from human blastocysts: somatic differentiation in vitro,” Nat. Biotechnol, 2000; 18: 399-404.
[8]Shamblott, M.J., Axelman, J., Wang, S., Bugg, E.M., Littlefield, J.W., Donovan, P.J., Blumenthal, P.D., Huggins, G.R. and Gearhart, J.D., “Derivation of pluripotent stem cells from cultured human primordial germ cells,” PNAS, Nov 1998; 95: 13726 - 13731.
[9] Shamblott, M.J., Axelman, J., Littlefield, J.W., Blumenthal, P.D., Huggins, G.R., Cui, Y., Cheng, L., and Gearhart, J.D., “Human embryonic germ cell derivatives express a broad range of developmentally distinct markers and proliferate extensively in vitro,” PNAS, Jan 2001; 98: 113 - 118.
[10]T Vernet, E Ziomek, A Recktenwald, JD Schrag, C de Montigny, DC Tessier, DY Thomas, and M Cygler, ”Cloning and expression of Geotrichum candidum lipase II gene in yeast. Probing of the enzyme active site by site-directed mutagenesis,” J. Biol. Chem.,1993; 268: 26212 - 26219.
[11] Weissman, I.L., ”Stem cells: units of development, units of regeneration, and units in evolution,” Cell, 2000; 100: 157-168.
[12]Givan A. 2001. Flow Cytometry: First Principles. New York: Wiley-Liss. 273p
[13]Moore, Keith L., “The developing human: clinically oriented embryology—5th ed,” W.B. SAUNDERS COMPANY, 1993
[14]Owens MA, Loken MR. 1995. Flow Cytometry: Principles for Clinical Laboratory Practice. New York: Wiley-Liss. 288p.
[15]http://stemcells.nih.gov/index.asp
[16]http://stemcells.nih.gov/info/scireport/chapter4.pdf
[17]http://www.bioteach.ubc.ca/MolecularBiology/FlowCytometry/
[18] http://www.ncbi.nlm.nih.gov/prow/iwhlda/iwhlda.htm
[19] http://www.ncbi.nlm.nih.gov/prow/guide/45277084.htm
[20] Molecular Probes Handbook:http://www.probes.com/handbook/
[21] Roederer M. 1997. Conjugation of monoclonal antibodies: http://www.drmr.com/abcon/
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