臺灣博碩士論文加值系統

為提升紅外光感測器測取負電胺基酸之靈敏度與選擇性，本研究提出以交聯法製備高極性感測層，利用感測層形成之網狀結構提昇感測層之水穩定性，亦利用感測層之正電性以及可酸鹼調控分子結構之特性得到選擇性測取麩胺酸之目的。製備出之新型感測層經測試其基本性質後，結果顯示此類感測層具有極佳之水穩定性，在pH值3~9的範圍內感測層無明顯之光譜變動，為進一歩瞭解此感測層之測量特性，研究中先篩選麩胺酸、酪胺酸以及精胺酸為指標分子，分別探討樣品溶液中pH值對感測訊號之影響。結果顯示在鹼性下，由於胺基酸易形成帶負電性容易測得，且以帶負兩價胺基酸效果最好。但在中性下，僅麩胺酸能有較佳之訊號，顯示此感測器能選擇性測取高解離性胺基酸的能力。為進一歩最佳化感測層，研究中亦探討交聯劑成份以及孔洞劑添加對感測性之影響。結果顯示交聯劑成份僅影響其水穩定性，對感測性質影響不大，而孔洞劑之添加對感測性有正面的幫助，且以直鏈型孔洞劑效果最好。綜合而言，本研究成功建立新型高極性紅外光感測改質層且成功應用至胺基酸之量測上。

Infrared spectroscopy provides a simple but powerful method to detect organic compounds. However, the detection of high polar compounds, such as negatively charged amino acid, is limited due to the high polarity and the negatively charged characteristics. In order to improve the selectivity and the sensitivity to the negatively charged amino acids, in this investigation, high polarity amine compounds was successfully immobilized on the internal reflection element by cross-linking method. The prepared sensing phase shows no obvious variation between pH=3 to 9 in aqueous solution. Besides, the network structure of the prepared sensing phase provides high water stability and the positively charged character of the sensing phase makes it possible to detect negatively charged amino acids selectively. Different kinds of amino acids are detected in different pHs to find the proper detecting condition. The results show that under the alkaline condition, both neutral and negatively charged amino acids can be detected by the amine cross-linked sensing phase; under the neutral or slightly acidic conditions, only negatively charged amino acids can be attracted into the sensing phase. For positively charged amino acids, repulsion will be occurred so that positively charged amino acids will not be detected by the amine cross-linked sensing phase in any pH condition.
Furthermore, to get the optimal formula in synthesizing the amine cross-linked sensing phase, factors, such as different porogenic agents, molar ratio of tri-(2-amino ethyl)-amine and cross-linking agent, molar ratio of cross-linking agent and porogenic agent, are investigated. The results indicate that adding porogenic agents, especially aliphatic compounds, will increase the signal of the sample molecules apparently. Besides, the cross-linking agent will facilitate the water stability of the sensing phase but will not contribute in drawing sample molecules. Finally, under the optimal condition, the linear range in detecting glutamic acid is between 5 to 50 micro mole and the limit of detection is ca. 2 micro mole under the pH=6 where provides the best selectivity.

中文摘要………………………………………………………………Ⅰ
英文摘要………………………………………………………………Ⅱ
謝誌……………………………………………………………………Ⅲ
總目錄…………………………………………………………………Ⅳ
圖表目錄………………………………………………………………Ⅴ
第一章 序論…………………………………………………………1
1-1前言 ………………………………………………………………1
1-2胺基酸檢測方法之回顧 …………………………………………2
1-3減弱式全反射法(ATR)原理簡介…………………………………3
1-4交聯性紅外光感測層設計原理介 ………………………………7
第二章 實驗部份 ……………………………………………………11
2-1實驗儀器與器材 …………………………………………………11
2-1-1FT-IR主機與樣品槽設計 ………………………………………11
2-1-2 其他器材 ………………………………………………………11
2-2實驗藥品……………………………………………………………13
2-3 藥品配置 …………………………………………………………14
2-4交聯式感測層之製備………………………………………………16
2-4-1莫耳比控制型交聯感測層………………………………………16
2-4-2 孔洞劑控制型感測層 …………………………………………17
2-5測量樣品之流程……………………………………………………17
第三章 結果與討論……………………………………………………19
3-1交聯式感測層基本性質測試………………………………………19
3-1-1交聯式感測層之厚度控制探討…………………………………19
3-1-2交聯式感測層之水穩定性探討…………………………………21
3-1-3交聯式感測層對不同pH值探討 ………………………………23
3-1-3交聯式感測層的再生性探討……………………………………26
3-2交聯式感測層感測性質探討………………………………………28
3-2-1感測胺基酸行為探討……………………………………………28
3-2-2 pH值對感測胺基酸影響之探討………………………………36
3-2-3 莫爾比控制對感測性質之探討 ………………………………46
3-2-4 添加不同孔洞劑對感測性質之探討 …………………………52
3-2-4-1 添加不同官能基孔洞劑之量測速度…………………………52
3-2-4-2 添加不同莫耳比之1-氯戊烷孔洞劑分子之量測速度……….56
3-3選擇性感測之性質 ………………………………………………59
3-4標準曲線的建立 ………………………………………………61
3-5 結論………………………………………………………………63
第四章總結 ……………………………………………………………64
參考文獻………………………………………………………………65

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