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研究生:田惟升
論文名稱:木香之化合物成份Ji224-6,對人類卵巢腺癌細胞株引導凋亡作用機轉之研究
論文名稱(外文):Study on the mechanisms of Ji224-6 from saussurea lappa on cell apoptosis in OVCAR-3 cells
指導教授:簡靜香簡靜香引用關係
學位類別:碩士
校院名稱:國立陽明大學
系所名稱:生物化學研究所
學門:生命科學學門
學類:生物化學學類
論文種類:學術論文
論文出版年:2002
畢業學年度:90
語文別:中文
論文頁數:51
中文關鍵詞:細胞凋亡
相關次數:
  • 被引用被引用:0
  • 點閱點閱:215
  • 評分評分:
  • 下載下載:23
  • 收藏至我的研究室書目清單書目收藏:0
Ji224-6是由中藥木香萃取出來的一種環狀化合物。曾有報告指出相似結構之化合物能夠抑制人類白血球腫瘤細胞(HL-60)之生長。因此我們針對不同細胞株,以MTT assay測試Ji224-6對卵巢腺癌細胞株(OVCAR-3)的細胞毒性之後,發現細胞的存活率隨著Ji224-6濃度的增加有漸驅下降的現象,呈現出dose-response的關係。
DAPI assay,及Flow cytometry等實驗結果顯示,以濃度5 mg/ml Ji224-6處理OVCAR-3細胞24小時看到染色質濃縮、sub-G0/G1 peak之細胞凋亡特徵,同時發現PARP(poly-(ADP ribose polymerase)被cleavage的現象,證明此一毒殺效果造成OVCAR-3細胞凋亡。
進一步利用蛋白質體(Proteomic)的研究來探討Ji224-6誘導OVCAR-3細胞凋亡的機制,藉由二維電泳上所發現具差異性之蛋白以及西方點墨法的進一步確認,推測Ji224-6透過幾個不同的訊息傳遞途徑誘導細胞凋亡。

目錄
英文摘要----1
中文摘要----2
緒論--------3
材料與方法--10
實驗結果----23
討論--------27
參考文獻----34
圖表--------41
附錄--------46

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