臺灣博碩士論文加值系統

在本篇論文中我們架構了穩態頻域光子遷移系統的光學方法去量測生物組織的生理參數，它是使用波長從600到1100奈米的近紅外光並利用光傳播理論模型去精確地得知組織的吸收係數和散射係數，同時利用此吸收係數和散射係數可以用來計算生物組織的色團濃度，例如：帶氧血紅素、不帶氧血紅素、水、脂質…等。在論文中我們使用穩態頻域光子遷移系統並搭配擴散探頭去量化活體皮膚的光學性質，首先我們測試了頻域光子遷移系統的振幅和相位的穩定性，這可以使我們了解系統的性能以及限制，其次我們利用量測四種不同吸收的樣品來探討系統收到訊號的線性程度，再來我們使用頻域光子遷移系統量測六種不同吸收和不同散射的液態假體，最後我們結合穩態系統和頻域光子遷移系統，使用穩態頻域光子遷移系統去測量液體假體，並量化液態假體的色團濃度，除此之外我們還使用穩態頻域光子遷移系統對活體的外側前臂做量測，並和穩態系統的結果做比較，再加以量化的生理濃度，最後我們的研究結果驗證了穩態頻域光子遷移系統是一個能用來定量組織組成的快速非侵入式光學方法。

In this thesis, we demonstrate the use of optical method, steady state frequency domain photon migration system (SSFDPM), to determine physiological parameters of biological tissues. It uses near-infrared light (from 600 to 1100 nm) coupled with mathematical photon transport models to accurately determine optical absorption (µa) and reduced scattering (µs′) properties of tissues. Absorption coefficient (µa) and reduced scattering coefficient (µs′) can be used to determine the chromophore concentrations, such as oxygenated hemoglobin, deoxygenated hemoglobin, water, and lipid, of biological tissues. Here, we employed the diffusing probe with the SSFDPM technique to quantify the optical properties of in-vivo skin. First, we characterize the stability of the amplitude and phase of the frequency domain photon migration system (FDPM) so that we can understand the limitations of our system. Second, we prepare four samples of different absorption to study the system linearity. Third, we use FDPM system to measure six liquid phantoms of various absorption and scattering properties. Finally, we combine the steady state (SS) and FDPM which is called SSFDPM to measure the liquid phantom, and quantify the chromophore concentrations of liquid phantoms. In addition, we carry out SSFDPM measurements on the in-vivo dorsal forearm and show the quantitative physiological concentration and compare with SS measurements. Our study reveals that the SSFDPM system provides a fast and noninvasive way for tissue composition quantification.

Abstract (in Chinese) I
Abstract (in English) II
Acknowledgements III
Table of Content IV
List of Tables VI
List of Figures VII
List of Symbols X
Chapter 1 Introduction 1
1.1 Motivation 1
1.2 Background 3
1.3 Objective of This Study 6
Chapter 2 Theoretical Background 8
2.1 Beer-Lambert Law 9
2.2 Radiative Transfer Equation 12
2.3 Diffusion Theory 16
2.4 Boundary Conditions 19
2.5 Two-Layered Diffusion Model 23
Chapter 3 Materials and Methods 27
3.1 Steady State Frequency Domain Photon Migration System 28
3.2 Characterizing the Stability of the Amplitude and Phase of the FDPM System 31
3.3 Modified Two-Layer Geometry 33
3.4 Liquid Phantom 34
3.5 Optical Property Determination 38
Chapter 4 Results and Discussion 41
4.1 Stability and Instrument Response of FDPM System 41
4.1.1 Stability of FDPM System 41
4.1.2 Instrument Response of FDPM System 48
4.2 Liquid Phantom Measurements by FDPM System 51
4.3 Liquid Phantom Measurements by SSFDPM System 56
4.4 Chromophore Fitting by SSFDPM System 60
4.5 Skin Measurements by SSFDPM System 62
Chapter 5 Conclusion and Future Work 68
5.1 Conclusion 68
5.2 Future Work 70
References 71

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