臺灣博碩士論文加值系統

本論文利用連續式電漿，以層層交錯的方式沉積丙烯酸與苯胺兩種分別具有羧基與胺基之前驅物，製備含胺基與羧基之多層薄膜，於矽晶圓與聚苯乙烯兩種不同的基材，並利用物理化學方法探討電漿功率、沉積時間等電漿參數與前驅物沉積順序，對於所製備的多層薄膜之厚度、水濕潤性、表面化學官能基與元素組成等性質的影響。
本論文利用電漿聚合所製備之多層薄膜，培養L-929老鼠纖維母細胞進行生物相容性測試，由LDH分析方法定量結果顯示，以電漿製備最外層為20 W ppANI及內層為20 W ppAA之多層薄膜，具有最多的細胞貼附量，其中兩層與四層之多層薄膜：N69/A271以及 (N69/A271)2，其細胞貼附量較未改質之樣本增加101 %與116 %。利用光學顯微鏡與雷射掃描共軛焦分光光譜顯微鏡觀察L-929老鼠纖維母細胞於此形式之多層薄膜的生長情形，結果顯示細胞型態為纖維狀，代表所製備之多層薄膜提供細胞良好的生長環境，細胞具有優異的延展性。此外，亦將此多層薄膜參數改沉積於聚苯乙烯上，發現僅有四層結構之多層薄膜較未改質之聚苯乙烯具有較多的細胞貼附量，藉由此生物相容性測試結果，說明利用電漿聚合方法製備含胺基與羧基之多層薄膜，有助於提升材料之生物相容性且可應用於不同的基材，因此具有應用於生醫材料之潛力。

This thesis proposed the layer-by-layer plasma polymerization method to prepare multilayered thin films containing amine and carboxylic functionalities. The parameters for plasma polymerization for the deposition of aniline and acrylic acid precursors were investigated physical-chemically on the applied plasma power, deposition time, and the orders of precursor for deposition. Moreover, the deposited multilayered thin films were characterized in terms of film thickness, surface wettability, surface functionalities, and chemical compositions.
For the applications of the prepared multilayered thin films, L-929 mouse fibroblasts were directly cultivated on the samples to evaluate the biocompatibility. The results of LDH (lactate dehydrogenase) assay showed that the surfaces prepared by plasma polymerization with different top layer of functionalities revealed distinct responses to cell growth. Among all the samples, the two and four layered thin films, N69/A271 and (N69/A271)2, revealed the most improved cell density that 101 % and 116 % cell density were obtained when compared with the pristine surface. Moreover, the confocal microscopy images also clearly showed that the well extended cell morphology on N69/A271 and (N69/A271)2. In order to prove the applicability of the proposed method for preparing multilayered thin films, the same plasma polymerization parameters were applied on polystyrene for preparing multilayered thin films and the results confirmed that the four-layered multilayered thin films showed higher cell density in comparison with the pristine polystyrene. The overall results indicated that the proposed layer-by-layer plasma polymerization for preparing multifunctional thin films allow to promote biocompatibility and could be successfully applied different substrates for applications in biomaterials.

摘要 I
Abstract II
致謝 IV
目錄 VI
圖目錄 IX
表目錄 XIII
第一章 緒論 1
1.1 研究背景 1
1.2 研究目標 1
1.3 論文總覽 2
第二章 文獻回顧 3
2.1 多層薄膜 3
2.1.1 多層薄膜之特性及應用 3
2.1.2 多層薄膜之製備方法 4
2.1.3 多層薄膜之生物相容性探討 6
2.2 前驅物簡介 9
2.2.1 丙烯酸介紹 9
2.2.2 苯胺介紹 10
2.3 電漿簡介 11
2.3.1 電漿定義 11
2.3.2 電漿薄膜沉積 12
2.3.3 電漿聚合 12
2.3.3.1 電漿聚合理論 13
2.3.3.2 電漿聚合參數 15
第三章 實驗方法與儀器原理 17
3.1 實驗藥品 17
3.1.1 電漿聚合前驅物 17
3.1.2 細胞培養液所需藥品 17
3.1.3 製備LDH溶液所需藥品 18
3.2 實驗方法 18
3.2.1 射頻電漿系統 18
3.2.2 前驅物校正曲線 20
3.2.3 電漿聚合多層薄膜 20
3.2.3.1 基材準備 21
3.2.3.2 電漿聚合 21
3.2.3.3 電漿腔體清潔 22
3.2.4 物理性質與化學性質分析 22
3.2.5 細胞貼附測試 23
3.2.5.1 細胞培養 23
3.2.5.2 繼代培養 23
3.2.5.3 培養細胞於樣本之上 23
3.3 儀器原理及方法 24
3.3.1 橢圓偏光儀 24
3.3.2 接觸角量測儀 25
3.3.3 原子力顯微鏡 26
3.3.4 全反射式傅立葉紅外線光譜儀 27
3.3.5 化學分析電子能譜儀 27
3.3.6 統計學分析 (statistical analysis) 28
3.4 電漿聚合薄膜之流量校正與多層薄膜樣本之命名 29
第四章 結果與討論 33
4.1 電漿沉積最外層為ppAA之多層薄膜 33
4.1.1 薄膜之厚度 33
4.1.2 薄膜之靜態水接觸角 35
4.1.3 ATR-FTIR分析薄膜之表面化學官能基 36
4.1.4 ESCA分析薄膜之表面化學組成 37
4.2 電漿沉積最外層為ppANI之多層薄膜 40
4.2.1 薄膜之厚度 40
4.2.2 薄膜之靜態水接觸角 42
4.2.3 ATR-FTIR分析薄膜之表面化學官能基 43
4.2.4 ESCA分析薄膜之表面化學組成 45
4.3 含胺基與羧基之多層薄膜的生物相容性測試 48
4.3.1 LDH方法分析電漿沉積最外層為ppAA之多層薄膜細胞貼附量 48
4.3.2 以光學顯微鏡觀察細胞於電漿沉積最外層為ppAA之多層薄膜的生長情形.......... 49
4.3.3 LDH方法分析電漿沉積最外層為ppANI之多層薄膜細胞貼附量 51
4.3.4 以光學顯微鏡觀察細胞於電漿沉積最外層為ppANI之多層薄膜的生長情形.......... 53
4.4 電漿沉積最外層為20 W ppANI、內層為20 W ppAA之多層薄膜探討 56
4.4.1 AFM分析薄膜之表面形態與粗糙度 56
4.4.2 薄膜之動態水接觸角 58
4.4.3 薄膜之高解析C1S特性峰分析 60
4.4.4 以雷射掃描共軛焦分光光譜顯微鏡觀察細胞於薄膜之生長情形 63
4.4.5 薄膜沉積於聚苯乙烯基材 65
第五章 結論與未來展望 66
5.1 含胺基與羧基之多層薄膜的表面分析 66
5.2 含胺基與羧基之多層薄膜的生物相容性測試 67
第六章 參考文獻 68
附錄 (Q&;A) 73

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