臺灣博碩士論文加值系統

肥胖是一種複雜的代謝疾病，與2型糖尿病，心血管疾病，高血壓和高脂血症風險增加有關。在先前的研究，黃連根莖的甲醇萃取物顯示出具有抗脂肪形成活性。由於使用不同溶劑萃取所含的成分會有所不同，因此本篇研究比較黃連 (Coptidis Rhizoma) 的水 (CW) 和乙醇 (CE) 萃取物對3T3-L1脂肪細胞形成的影響。我們發現CW和CE都會抑制3T3-L1脂肪細胞的脂肪生成及其相關基因表現，例如：三酸甘油脂含量，peroxisome proliferator-activated receptor γ (PPARγ)，CCAAT/enhancer-binding protein α (C /EBPα) 和脂肪酸合成酶，CE對3T3-L1細胞具有比CW更高的細胞毒性。通過HPLC分析顯示CW可以萃取出較多的小蘗鹼。在考量能有效而方便萃取有效成分與安全性下，我們認為黃連水萃取物比酒精萃取更有利於肥胖症的治療。

Obesity is a complex metabolic disorder related to an increased risk of common and serious human diseases, including type 2 diabetes mellitus, cardiovascular diseases, hypertension, and hyperlipidemia. Previously, the methanol extract from the rhizome of the Chinese medicinal plant Coptis chinensis Franch. has been shown to exhibit anti-adipogenic activity. Given that extraction by using different solvents leads to obtain diverse constituents. Accordingly, the effect of water (CW) and ethanol (CE) extracts of Coptidis Rhizoma on 3T3-L1 adipogenesis were compared in this study. We investigated both CW and CE on gene expression during the processes of adipogenesis and lipogenesis in 3T3-L1 cells. The result showed CE with higher cytotoxicity than CW in 3T3-L1 cells. Both CW and CE displayed similar potency on inhibition of adipogenesis and its related gene expression such as triglyceride content, peroxisome proliferator-activated receptor gamma (PPARγ), CCAAT/enhancer-binding protein alpha (C/EBPα), and fatty acid synthase in 3T3-L1 adipocytes. Using HPLC analysis, we compared the berberine with extracts of CW and CE and found extraction by water has a higher yield of berberine than by ethanol. These findings suggested that water extract of Coptidis Rhizoma is more beneficial to the treatment of obesity regarding safety, convenient preparation, and high yield of berberine.

中文摘要 1
ABSTRACT 2
第一章 緒論 8
第一節 文獻回顧 8
一、 肥胖 8
二、檢測的方法 8
三、藥物治療方法 9
四、研究藥物 21
五、研究動機及目的 25
第二章 實驗材料與方法 26
第一節 實驗材料 26
一、 細胞株 26
二、 抗體 26
三、 藥品 27
第二節 實驗方法 27
一、 細胞培養 (Cell culture) 27
二、 細胞數計算 (Cell count) 28
三、 細胞毒殺性試驗 (Cytotoxicity assay) 28
四、 三酸甘油脂濃度測試 29
五、 西方墨點法 (Western blotting) 30
六、 HPLC 錯誤! 尚未定義書籤。
七、統計方法 33
第三章 研究結果 36
第一節 19種中藥水萃取物對3T3-L1小鼠脂肪前驅細胞之三酸甘油脂含量的影響 36
第二節 19種中藥水萃取物對3T3-L1小鼠脂肪前驅細胞之脂質堆積的影響 37
第三節 19種中藥水萃物對3T3-L1小鼠脂肪前驅細胞之細胞毒殺性試驗 38
第四節 黃連水萃與乙醇萃取物對3T3-L1之三酸甘油脂含量的影響 39
第五節 黃連水萃與乙醇萃取物對3T3-L1之細胞毒殺性 40
第六節 黃連水萃與乙醇萃取物對3T3-L1脂質分化之蛋白質表現的影響 41
第七節 黃連水萃與乙醇萃取物對3T3-L1早期脂質生成之蛋白質表現的影響 42
第八節 比較黃連水和乙醇萃取物含有多少的小檗鹼 43
第四章 研究討論 57
第一節 黃連水萃與乙醇萃取物對3T3-L1脂肪細胞具有抑制脂質生成效果 57
第二節 黃連水萃與乙醇萃取物對3T3-L1脂肪分解效果 58
第三節 黃連中主要化合物小檗鹼的萃取率 59
第四節 未來的研究方向與結論 60
第五章 參考文獻 61

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