臺灣博碩士論文加值系統

中文摘要...................................................Ⅰ
英文摘要...................................................Ⅱ
誌謝...................................................Ⅲ
目錄...................................................Ⅳ
圖目錄..................................................Ⅵ
第一章緒論
1-1前言.................................................1
1-2文獻回顧.................................................1
1-3研究動機...............................................2
1-4論文架構..............................................3
第二章相關理論探討
2-1聚合酶連鎖反應(PCR) ...............................3
2-2南方點墨法..........................................5
2-3 DNA雜合反應........................................5
2-4 PMMA黏彈材料分析.................................5
2-4-1材料特性模型.........................................5
2-4-2應變、應力參數方程式..................................6
2-5螢光激發原理..........................................7
2-6 CO2雷射加工機....................................9
2-7 壓力感測器.......................................9
第三章實驗與研究方法
3-1實驗用晶片設計與製造....................................10
3-1-1晶片設計............................................11
3-1-2晶片製造..........................................12
3-2拉升實驗..............................................13
3-3生物實驗架構及南方點墨法...................................14
3-3-1生物樣本介紹.........................................14
3-3-2 DNA點漬法(Dot blot)與南方點墨法(Southern blot)......16
3-4生物晶片實驗架構.....................................17
3-4-1生物實驗原理.........................................17
3-4-2生物實驗步驟.........................................17
3-4-3 晶片空腔壓力量測.....................................18
3-5生物晶片檢測方式....................................19
3-5-1 倒立式螢光檢測系統...................................19
3-5-2雷射激發訊號檢測.....................................20
第四章實驗結果
4-1SEM掃描式電子顯微鏡分析...............................21
4-2應變應力量測......................................22
4-2-1不同溫度下材料的應力鬆弛曲線.........................22
4-2-2COMSOL 有限元素法數值分析原理.........................25
4-2-3 COMSOL 模擬分析......................................26
4-3 南方點墨法..........................................29
4-3-1 DNA雜合反應.........................................29
4-3-2 螢光染劑對DNA、Probe染色及沖刷......................30
4-4倒立式螢光檢測.....................................32
4-4-1不同種類沖刷液對DNA沖刷結果.....................32
4-4-2不同分子量DNA沖刷結果...............................33
4-4-3沖刷液A、B對DNA、Probe-HEX沖刷.................34
4-4-4螢光染劑對 ET996-HEX 化學性質的影響..................35
4-4-5 DNA雜合反應.........................................35
4-5單通道雷射激發螢光檢測..............................37
4-5-1光譜儀螢光波長量測...................................37
4-5-2 電訊號檢測結果分析...................................38
第五章 結論與未來展望
5-1 結論.................................................39
5-2 未來展望.........................................40
第六章 參考文獻
第七章 附錄
7-1 PCR 步驟..........................................41
7-2電泳裝置與檢驗....................................42
7-3沖刷液調配.......................................43
7-4 Labview 讀取影像檔 .................................43
7-5生物溶液調配.....................................44

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