臺灣博碩士論文加值系統

背景：血脂異常會嚴重地威脅到我們的健康且造成龐大的醫療支出，除了服用藥物，如何預防或尋求整合性療法是目前的研究趨勢，例如補充常見的植物萃取物。目的：使用一個適當的動物模組－黃金敘利亞倉鼠，探討一草本複合補充品 (含有可可、咖啡、綠茶、及山竹，CCGG) 對於調節血脂、血糖指標及促發炎細胞激素之作用。方法：40隻雄性倉鼠隨機分作五組：(1) 正常飲食控制組、(2) 高膽固醇飲食組、(3) 高膽固醇飲食並管餵1倍之CCGG (311 mg/kg/d)、(4) 高膽固醇飲食並管餵2倍之CCGG (622 mg/kg/d)、(5) 高膽固醇飲食並管餵5倍之CCGG (1555 mg/kg/d)，在實驗結束後蒐集血清、組織、糞便檢體以便陸續分析。結果：經過六週的實驗，發現CCGG補充劑可顯著地減少血清與肝臟脂肪含量，且有劑量相關之效應。此外，介入後亦增加糞便脂肪量的排出。再者，與血脂異常有關聯的胰島素平衡之指標與促發炎細胞激素之濃度皆有得到明顯地改善。此外，藉由監測血清生化指標與觀察主要臟器之切片，結果發現補充CCGG 6週後並未有毒性產生。結論：CCGG補充品的介入可改善高血脂、胰島素阻抗性、脂肪肝、及發炎反應。

Background: Dyslipidaemia severely threatens health and is associated with exorbitant medical &;#8232;expenses. Instead of taking medicine, integrative therapy by plant extract is the current research trend. Purpose: To understand the synergic beneficial effects of a herbal complex supplement (comprising cocoa, coffee, green tea, and garcinia; CCGG) on regulating lipid profiles, glycaemic markers, and related proinflammatory cytokines by using an appropriate animal model, the golden Syrian hamster. Methods: A total of 40 male hamsters were randomly assigned to five groups: (1) vehicle control, (2) high-cholesterol diet control, (3) high-cholesterol diet of 311 mg/kg/d of CCGG, (4) high-cholesterol diet of 622 mg/kg/d of CCGG, and (5) high-cholesterol diet of 1555 mg/kg/d of CCGG. At the end of an experiment, blood, tissue and faecal samples were collected for further analysis.&;#8232;Results: After 6 wk of treatment, CCGG supplementation significantly reduced serum and hepatic lipid levels with dose-dependent effects. In addition, an increase in excretion of faecal lipids was observed after supplementation. Furthermore, the homeostasis model assessment of insulin resistance index and serum proinflammatory cytokine levels involved in dyslipidaemia was markedly improved. In addition, by monitoring biochemical parameters as well as histopathology of major tissues, no toxic results were observed after 6-week consuming CCGG. Conclusion: Dietary CCGG supplementation may exert potential effects on ameliorating hyperlipidaemia, insulin resistance, liver steatosis, and inflammation.

第一章 緒論 1
第一節 研究背景 1
第二節 研究目的 3
第二章 文獻回顧 4
第一節 血脂異常的分類與定義 4
第二節 血脂異常的生理生化機轉 7
第三節 血脂異常常見的膳食補充劑之使用與治療方式 10
第四節 血脂異常伴隨的發炎反應 15
第五節 補充劑的介紹 17
第六節 動物模式的介紹 22
第三章 材料與方法 23
第一節 飲食及化學材料 23
第二節 實驗動物及處理 25
第三節 血清檢體之生化分析 28
第四節 肝臟脂肪含量 29
第五節 糞便中脂肪含量 29
第六節 臟器組織病理 30
第七節 統計分析 30
第四章 結果與討論 31
第一節 CCGG對體重、臟器重量以及飲食攝取之影響 31
第二節 CCGG對血脂之影響 31
第三節 CCGG對血糖與胰島素阻抗之影響 32
第四節 CCGG對血清促發炎細胞激素之影響 32
第五節 CCGG補充對血清生化指標之影響 33
第六節 CCGG補充對肝臟及糞便脂質含量之影響 33
第七節 CCGG補充對組織切片之影響 34
第五章 討論 35
第一節 CCGG對倉鼠脂肪、醣類指標及重量之影響 35
第二節 CCGG對發炎反應之影響 37
第三節 CCGG之劑量比較 39
第六章 結論 41
參考文獻 42

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