臺灣博碩士論文加值系統

本研究利用單軸靜電紡絲製備具有快速溶解特性的電紡絲奈米纖維墊 ，以及含有導電高分子的電紡奈米纖維絲，用於細胞培養以及快速藥物釋放。
以不同的高分子聚碸(PES）與聚苯乙烯(PS）混和二甲基甲醯胺(DMF）作為前驅物溶液，再摻雜不同的導電高分子PEDOT或PANI，以靜電紡絲製備大量奈米纖維；再以聚乙烯吡咯烷酮(PVP)混合酒精作為前驅液，並以維他命C作為目標分子混溶，並以靜電紡絲法製備奈米纖維不織布 (non woven)，接著進行細胞培養及藥物釋放的實驗，通過場發射電子顯微鏡( FE-SEM )、熱重損失分析儀(TGA)、熱機械分析儀(TMA)及水滴角測試儀對奈米纖維特性進行分析，並探討細胞生長與藥物釋放的情形。
實驗結果顯示，藥物釋放的奈米纖維絲線，在經過置於空氣中不同的時間，會因為水氣及其本身的性質獲得收縮的能力，經過長時間收縮的奈米纖維絲會失去其奈米的性質，進而導致溶解的速度降低，進而可以控制其釋放速率。而細胞培養的電紡絲試片，可以發現兩種高分子都具有一定的生物相容性，其中PES可以提供更好的細胞生長空間，而兩者在添加了導電高分子後皆有更為良好的細胞生長能力，其中PEDOT能夠使細胞更為良好的生長能力。

In this study, nanofiber mats with rapid dissolution characteristics and nanofibers containing conductive polymers were prepared by uniaxial electrospinning for cell culture and rapid drug release.
Firstly, precursor solution was prepared: the polysulfone (PES) and Polystyrene (PS) dissolved in Dimethylformamide (DMF) and doping conductive polymer poly(3,4-ethylenedioxythiophene) polystyrene sulfonate (PEDOT) and Polyaniline (PANI) to make sure the concentration of solution in appropriate using single-nozzle electrospinning to produce the nanofibers, and this nanofibers used in macrophages culture.
Secondly, precursor solution was prepared: the polyvinylpyrrolidone (PVP) dissolved in ethanol and doping Vitamin C to make sure the concentration of solution in appropriate using single-nozzle electrospinning to produce the nanofibers, and this nanofibers used in drug delivery.
The characterized of nanofibers, following instruments were used: Field Emission Scanning Electron Microscope (FE-SEM), Thermogravimetric Analyzer (TGA), Thermal Mechanical Analyzer (TMA), FE-SEM were used to explore the morphology and diameter of nanofibers. Water droplets were used to analyze the properties of nanofibers. Finally, Optical microscope used for macrophages culture in nanofibers.

摘 要 I
Abstract II
誌謝 III
總目錄 V
表目錄 VIII
圖目錄 IX
第一章　緒論 1
1.1前言 1
1.2研究目的與動機 2
第二章　文獻回顧 3
2.1靜電紡絲歷史發展 3
2.2 靜電紡絲介紹 5
2.2.1 靜電紡絲基本原理 6
2.2.2 靜電紡絲基本理論 7
2.3 靜電紡絲方法與影響因子 8
2.3.1 高分子的分子量與溶液濃度 8
2.3.3工作距離 12
2.3.4高分子溶液的導電性和表面張力 13
2.3.5溫溼度對高分子溶液的影響 13
2.3.6施加的電壓強度(單位kv) 14
2.3.7注射幫浦推進速率(mL/min) 15
2.3.8高分子聚合物的性質 16
2.3.9.電場的影響因子 18
2.4靜電紡絲相關應用 20
2.4.1應用於燃料電池上 21
2.4.2應用於發光二極體上面 23
2.4.3應用於場效電晶體(FET)上 24
2.4.4應用在光催化效應上 25
2.4.5應用於生物醫學 26
2.4.6應用於藥物釋放上 27
2.5組織工程(Tissue Engineering) 30
2.5.1 支架 31
2.5.2 細胞 31
2.5.3訊息因子 32
第三章　實驗方法 33
3.1研究方法 33
3.2實驗材料 34
3.3實驗試片製備 37
3.3.1 分析試片製備 37
3.3.1.1細胞培養試片製備 37
3.3.1.2藥物釋放試片製備 39
3.4實驗儀器 40
3.4.1靜電紡絲設備( Electro-spinning ) 40
3.4.2場發射電子顯微鏡( FE-SEM ) 41
3.4.3熱重分析儀 (TGA) 42
3.4.4原子力顯微鏡（AFM） 43
3.4.5熱機械分析儀(TMA) 44
3.4.6接觸角量測儀 45
第四章　結果 46
4.1靜電紡纖維表面形貌與線徑分布 46
4.2靜電紡纖維表面形貌與細胞分佈 61
4.3靜電紡纖維進行穿刺實驗溫度曲線圖 67
第五章　討論 70
5.1 溶液濃度對纖維的影響 70
5.2 時間對於奈米纖維形貌尺寸變化影響 71
5.3 時間對於試片溶解速率的影響 80
5.4 奈米纖維結構對於細胞培養的影響 81
第六章　結論 85
第七章　參考文獻 86

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