臺灣博碩士論文加值系統

阿魏酸(ferulic acid)是一種中草藥之主要活性成分，具有多種生物活性，包括抗氧化、抗發炎、抗過敏、抗癌和抑菌等。紅黴素(erythromycin)是一種大環內酯類的抗生素，普遍用於治療細菌感染。本研究將水膠球包覆阿魏酸和紅黴素用於口服藥物製劑。本實驗採用海藻酸鈉(sodium alginate)/關華豆膠(guar gum)混摻均勻溶液，滴入氯化鈣溶液，利用鈣離子來交聯，製備成酸鹼敏感型水膠球。探討此海藻酸鈉/關華豆膠水膠球在不同pH環境下其膨潤行為。在模擬小腸液(simulated intestinal fluid)及模擬結腸液(simulated colonic fluid)中測試包覆阿魏酸水膠球的持續藥物釋放量。包覆阿魏酸水膠球其總酚含量及抗氧化活性利用Folin-Ciocalteu比色法、DPPH自由基清除率及螯合亞鐵離子能力等方法評估。以MTT比色法評估，海藻酸鈉/關華豆膠/阿魏酸水膠球可允許纖維母細胞L929貼附與增生，說明水膠球具有良好的生物相容性。海藻酸鈉/關華豆膠/紅黴素水膠球其抑菌能力經由實驗顯示，對於革蘭氏陰性大腸桿菌(E. coli)及革蘭氏陽性金黃色葡萄菌(S. aureus)有良好的抗菌活性。綜合以上結果，包覆阿魏酸和紅黴素之海藻酸鈉/關華豆膠水膠球可應用於口服藥物載體。

Ferulic acid, a major active constituent of Chinese medicinal herbs, exhibits a variety of biological activities such as antioxidant, anti-inflammatory, anti-allergic, anti-cancer, and antibacterial effects. Erythromycin, a macrolide antibiotic, is commonly used in treatment of many bacterial actions. Ferulic acid-loaded and erythromycin-loaded hydrogel beads as oral delivery formulations were studied. In this work, the homogeneous sodium alginate/guar gum solution was dropped into calcium chloride solution, crosslinked by calcium ions, and the pH-sensitive hydrogel beads were formed. The swelling behaviors of sodium alginate/guar gum hydrogel beads were studied at different pH environment. The sustained release profiles of ferulic acid-loaded hydrogel beads were investigated in simulated intestinal fluid (SIF) and simulated colonic fluid (SCF). The total phenolic content and antioxidant activity of Ferulic acid-loaded hydrogel beads were evaluated using the Folin-Ciocalteau method, 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity and ferrous ion chelating ability assays. The MTT assays show that the sodium alginate/guar gum/ferulic acid hydrogel beads have good biocompatibility, which allow adhesion and proliferation of the L929 fibroblast cells. Finally, the antibacterial actions against gram-negative (E. coli) and gram-positive (S. aureus) bacteria of sodium alginate/guar gum/erythromycin hydrogel beads have been investigated. These results indicated that ferulic acid-loaded and erythromycin-loaded sodium alginate/guar gum hydrogel beads show a promising application in drug carriers.

Abstract i
摘要 ii
目錄 iii
圖目錄 v
表目錄 vii
第一章 前言 1
第一節 研究背景 1
第二節 研究目的 2
第二章 文獻回顧 5
第一節 藥物釋放 5
第二節 智慧型水膠 10
第三節 海藻酸鈉 19
第四節 關華豆膠 21
第五節 阿魏酸 23
第三章 實驗材料與方法 26
第一節 實驗藥品 26
第二節 實驗儀器 27
第三節 實驗流程 28
第四節 實驗方法 29
第四章 實驗結果與討論 38
第一節 物理性質分析 38
第二節 抗氧化能力分析 42
第三節 生物相容性 44
第五章 結論 48
第六章 參考文獻 50

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