臺灣博碩士論文加值系統

紅葡萄葉( vitis ainifera L.)廣泛生長於歐洲與美洲地區。紅葡萄葉具備抗氧化活性，本研究的目的在探討紅葡萄葉成分，以高效能液相層析儀(High-performance liquid chromatography )分析及鑑定，並測定其總酚含量、總黃酮含量、清除DPPH能力、還原力、清除超氧陰離子能力、清除ABTS．+自由基能力以及美白測試。HPLC分析結果顯示，紅葡萄葉含有resveratrol、kaempferol、epicatechin、quercetine、chlorgenic acid、rutin、gallic acid等七種成分[1]。抗氧化能力結果顯示，紅葡萄葉酚類化合物含量為2.8 mg of GAE/g，清除DPPH自由基為74.30 % ，總黃酮含量為 0.6827 mg of quercetin/ mg，超氧陰離子清除能力IC50為0.1372 mg/mL。美白試驗部分IC50為0.16175 mg/mL。可知紅葡萄葉具有抗氧化及美白效果。

關鍵字：紅葡萄葉、抗氧化、高效液相層析、抑制酪胺酸酶

vitis ainifera L. is extensively grown in Europe and America region. The V. ainifera has the antioxidant activity. The purpose of the this research is inquiring into V. ainifera 　composition and
measures with high-performance liquid chromatography. Total phenolic contents、total flavonoid content、DPPH free radical scavenging ability、reducing power、clear superoxide anion capacity、ABTS･+ free radical capacity and tyrosinase inhibited activity. There are sevsn compositions in V. ainifera including resveratrol、kaempferol、 epicatechin、 quercetine、chlorgenic acid、rutin、gallic acid. There results of antioxidant capacity of phenolic content is 2.8 mg of GAE/g , DPPH radical scavenging is 74.30 % ; total flavonoid content is 682.71 μg of quercetin/mg 、IC50 of superoxide anion scavenging is 0.14 mg/mL. IC50 of tyrosinase inhibited activity : 161.75 μg/mL. Therefore V. ainifera has both antioxidant and whitening effect.

Keywords: vitis ainifera L.、Antioxidant Activity、High-performance liquid chromatography,、Inhibited tyrosinase,

目錄
中文摘要………………………………………………………………...Ⅰ
英文摘要………………………………………………………………...Ⅱ
目錄…………………………………………………………………………...Ⅲ
表目錄………………………………………………………………………...Ⅴ
圖目錄………………………………………………………………………...Ⅵ
第一章 前言…………………………………………………………………..1
第一節 自由基探討…………………………………………………….2
第二節 美白……………………………………………………………….3
第三節 美白機轉………………………………………………………….5
第四節 紅葡萄葉成分探討……………………………………………....6
第二章 實驗方法……………………………………………………………..9
2-1 萃取實驗方法………………………………………………………...9
2-2 高效液相層析儀之成分分析………………………………………10
2-3抗氧化能力測定……………………………………………………..16
第三章 結果與討論…………………………………………………………31
第一節 紅葡萄葉成分分析………………………...............................31
第二節 紅葡萄葉抗氧化能力測定結果……………………………….38
第四章 結論………………………………………………………………….46
第五章 參考文獻……………………………………………………………48

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