臺灣博碩士論文加值系統

在本研究中，利用微機電製程技術製造出一個操作方式簡單的
P x Q管道[1](P:流體樣本入口槽數目，Q:流體樣本出口槽數目)微流體晶片，本研究中使用P=3，Q=2之晶片。並且利用電壓驅動產生電滲流，在晶片的前段達成流體聚焦(flow focusing)的效應。晶片中段主要是利用電壓的配置以及時間的控制，再經由一邏輯算式算出要注入樣本傳輸管道(晶片中段管道)的流體樣本長度，使流體樣本能以不同長度依序流入主要管道。最後，在晶片末段，不同長度的流體樣本可以各別的被傳送入不同的出口管道。即是以排列的形式於樣本傳輸管道傳輸不同長度的流體樣本，接著再將不同長度的流體樣本，各自加以分離，達成序列式注射(sequential injection)的功能。

This study proposes a technique for the sequential injection of electroosmotically-driven sample flows in microfluidic devices. The study commences by analyzing the buffer length required to prevent diffusion between successive sample plugs and then develops an analytical formulation to determine the operating parameters guaranteed to ensure deposition of the complete sample plug in the designated outlet reservoir. It is shown that by an appropriate manipulation of the voltages applied to the various inlet and outlet channels of the microfluidic device, samples of different lengths can be delivered automatically and continuously to the specified outlet reservoirs. It is demonstrated experimentally that the injection performance of the microchip can be enhanced via the application of a voltage to the non-receiving reservoir during the injection process to prevent sample leakage. Finally, the experimental and numerical results indicate that the injection performance can be further improved by optimizing the geometrical arrangement of the outlet channels.

中文摘要 I
英文摘要 II
誌謝 III
目錄 V
表目錄 VII
圖目錄 VIII符號說明 X
第一章 緒論
1-1前言 1
1-2微流體晶片 2
1-3電滲流關於流體操控的重要性 3
1-4研究動機與目的 4
1-5電滲流的應用及文獻回顧 5
第二章 序列式注射原理
2-1序列式樣品流體產生之方式 9
2-2置入緩衝液於兩流體樣本間之方式 10
2-3序列式注射之邏輯 12
2-4以類比電路方式解釋電壓配置情形 16
第三章 晶片製作
3-1製作光罩 19
3-2晶片基材 19
3-3晶片製作過程 20
第四章 實驗方法
4-1實驗設備與前置 24
4-2實驗操作過程 26
第五章 結果與討論
5-1樣本流聚焦 28
5-2D line的重要性 29
5-3D line與水平線的交角 30
5-4製造不同長度樣本 33
5-5緩衝液擴散對於樣本傳輸之影響 34
5-6樣本洩漏之解決方式 35
第六章 總結與展望
6-1總結 36
6-2展望 37
參考文獻 38

表目錄
2.1微流體晶片電壓設定表示方式 43

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