臺灣博碩士論文加值系統

異位性皮膚炎是犬隻常見的皮膚疾病，患犬接受環境過敏原的刺激後產生過量免疫球蛋白E (IgE)進而誘發一連串過敏反應。在人類的研究，總血清IgE濃度上升，為一異位性皮膚炎發病的風險因子，同時總血清IgE濃度可做為異位性皮膚炎診斷的指標之ㄧ；此外也發現異位性皮膚炎患者在接受皮黴菌治療後，過敏的嚴重程度較為緩和。此篇論文包含兩個研究，第一篇比較異位性皮膚炎患犬及健康犬隻的總血清IgE濃度、總血清IgE濃度和異位性皮膚炎嚴重程度指數(CDESI)的相關性、抗原特異性IgE相對於皮內測試的敏感性及特異性；第二篇則調查異位性皮膚炎患犬體表皮屑芽孢菌及皮黴菌的分布、比較直接鏡檢法和黴菌培養在診斷皮黴菌症的一致性。第一篇研究結果指出，總血清IgE濃度在規律預防內外寄生蟲的犬隻，可作為初步篩選異位性皮膚炎的診斷指標，同時可評估其嚴重程度，而抗原特異性IgE相較於皮內過敏原測試，可能會低估其誘發過敏原；第二篇研究結果發現，台北地區異位性皮膚炎患犬，皮黴菌症盛行率高於皮屑芽孢菌皮膚炎，而直接鏡檢法相較於黴菌培養在皮黴菌症的診斷，有較高的敏感性。

Atopic dermatitis is a common skin disease in dogs which is sensitized to environmental antigens and produces high level of immunoglobulin E (IgE) antibodies. In human patients, increasing total serum IgE concentrations represents a major empiric risk factor for development of atopic disease and was used as a diagnostic indicator of atopic dermatitis. Furthermore, severity of atopic dermatitis was found to be alleviated with management of dermatophytosis in atopic patients. This thesis includes 2 studies, the first study reported total serum IgE concentrations atopic dogs and dogs free from atopic dermatitis, correlation between total serum IgE concentrations and modified canine atopic dermatitis extent and severity index (CADESI), and sensitivity and specificity of allergen-specific IgE in detecting offending allergens against intradermal skin tests. The second study was to investigate the prevalence of Malassezia spp. and dermatophytes in atopic dogs and concordance between results of direct microscopic examination and fungal culture in diagnosis of dermatophytosis. Results of the first study suggested that total serum IgE concentrations could be a screening test and determine severity of atopic dermatitis in dogs received regular parasitic prophylactics, however the use of allergen-specific IgE tests might under-detect offending allergens. Results of the second study showed that the prevalence of dermatophytosis was higher than Malassezia dermatitis in atopic dogs in Taipei area. Comparing to fungal culture microscopic examination exhibited a higher sensitivity in diagnosis of dermatophytosis.

口試委員會審定書 i
Acknowledgment ii
Abstract iii
中文摘要 iv
Table of contents v
List of Tables vi
List of Figures vii
Chapter 1 General Introduction 1
References 5
Chapter 2 Studies of total serum IgE and allergen-specific
IgE tests in atopic dogs 11
References 19
Chapter 3 Studies of Malassezia pachydermatis and
dermatophytes isolated from skin surface
of atopic dogs 34
References 40
Chapter 4 General discussion and conclusions 47

Chapter1
References
1.Reedy LM, Miller WH, Willemse T. Allergic skin diseases of dogs and cats. 2nd ed. London ; Philadelphia: W.B. Saunders, 1997.
2.Muller GH, Kirk RW, Scott DW, et al. Muller & Kirk''s small animal dermatology. 6th ed. Philadelphia: W.B. Saunders, 2001.
3.Saridomichelakis MN, Koutinas AF, Gioulekas D, et al. Canine atopic dermatitis in Greece: clinical observations and the prevalence of positive intradermal test reactions in 91 spontaneous cases. Vet Immunol Immunopathol 1999;69:61-73.
4.Griffin CE, DeBoer DJ. The ACVD task force on canine atopic dermatitis (XIV): clinical manifestations of canine atopic dermatitis. Vet Immunol Immunopathol 2001;81:255-269.
5.Halliwell RE, DeBoer DJ. The ACVD task force on canine atopic dermatitis (III): the role of antibodies in canine atopic dermatitis. Vet Immunol Immunopathol 2001;81:159-167.
6.Hill PB, Olivry T. The ACVD task force on canine atopic dermatitis (V): biology and role of inflammatory cells in cutaneous allergic reactions. Vet Immunol Immunopathol 2001;81:187-198.
7.Griffin CE, DeBoer DJ. The ACVD task force on canine atopic dermatitis (XIV): clinical manifestations of canine atopic dermatitis. Vet Immunol Immunopathol 2001;81:255-269.
8.Willemse A. Atopic skin disease : a review and a reconsideration of diagnostic criteria. J Small Anim Prac 1986;27:771-778.
9.Prèlaud P, Guaguére E, Alhaidari Z, et al. Reevaluation of diagnostic criteria of canine atopic dermatitis. Rev Med Vet 1998;149:1057-1064.
10.Olivry T, Guaguère E, Héripret D. Treatment of canine atopic dermatitis with misoprostol, a prostaglandin E1 analogue: an open study. J Dermatol Treatment 1997;8:243-247.
11.Juhlin L, Johansson GO, Bennich H, et al. Immunoglobulin E in dermatoses. Levels in atopic dermatitis and urticaria. Arch Dermatol 1969;100:12-16.
12.Wilkie JS, Yager JA, Eyre P, et al. Morphometric analyses of the skin of dogs with atopic dermatitis and correlations with cutaneous and plasma histamine and total serum IgE. Vet Pathol 1990;27:179-186.
13.Hill PB, Moriello KA, DeBoer DJ. Concentrations of total serum IgE, IgA, and IgG in atopic and parasitized dogs. Vet Immunol Immunopathol 1995;44:105-113.
14.Hill PB, DeBoer DJ. Quantification of serum total IgE concentration in dogs by use of an enzyme-linked immunosorbent assay containing monoclonal murine anti-canine IgE. Am J Vet Res 1994;55:944-948.
15.Schwartzman RM. Immunologic studies of progeny of atopic dogs. Am J Vet Res 1984;45:375-378.
16.Halliwell RE, Kunkle GA. The radioallergosorbent test in the diagnosis of canine atopic disease. J Allergy Clin Immunol 1978;62:236-242.
17.Racine BP, Marti E, Busato A, et al. Influence of sex and age on serum total immunoglobulin E concentration in Beagles. Am J Vet Res 1999;60:93-97.
18.Griot-Wenk ME, Busato A, Welle M, et al. Total serum IgE and IgA antibody levels in healthy dogs of different breeds and exposed to different environments. Res Vet Sci 1999;67:239-243.
19.Fraser MA, McNeil PE, Gettinby G. Studies of serum total immunoglobulin E concentrations in atopic and non-atopic dogs. Vet Rec 2003;152:159-163.
20.Zunic M. Comparison between IMMUNODOT tests and the intradermal skin test in atopic dogs. Vet Dermatol 1998;9:201-205.
21.Tarpataki N, Bigler B, Vajdovich P, et al. Comparison between an intradermal skin test and allergen-specific IgE-ELISA for canine atopic dermatitis. Schweiz Arch Tierheilkd 2008;150:117-122.
22.Park S, Ohya F, Yamashita K, et al. Comparison of response to immunotherapy by intradermal skin test and antigen-specific IgE in canine atopy. J Vet Med Sci 2000;62:983-988.
23.Mueller RS, Burrows A, Tsohalis J. Comparison of intradermal testing and serum testing for allergen-specific IgE using monoclonal IgE antibodies in 84 atopic dogs. Aust Vet J 1999;77:290-294.
24.Bond R, Collin NS, Lloyd DH. Use of contact plates for the quantitative coutures of Malassezia pathydermatis from canine skin. J Small Anim Prac 1994;35:68-72.
25.Nardoni S, Dini M, Taccini F, et al. Occurrence, distribution and population size of Malassezia pachydermatis on skin and mucosae of atopic dogs. Vet Microbiol 2007;122:172-177.
26.Prado MR, Brilhante RS, Cordeiro RA, et al. Frequency of yeasts and dermatophytes from healthy and diseased dogs. J Vet Diagn Invest 2008;20:197-202.
27.Bond R, Ferguson EA, Curtis CF, et al. Factors associated with elevated cutaneous Malassezia pachydermatis populations in dogs with pruritic skin disease. J Small Anim Pract 1996;37:103-107.
28.Jones HE, Reinhardt JH, Rinaldi MG. A clinical, mycological, and immunological survey for dermatophytosis. Arch Dermatol 1973;108:61-65.
Chapter2
References
1.Berg T, Johansson SG. IgE concentrations in children with atopic diseases. A clinical study. Int Arch Allergy Appl Immunol 1969;36:219-232.
2.Juhlin L, Johansson GO, Bennich H, et al. Immunoglobulin E in dermatoses. Levels in atopic dermatitis and urticaria. Arch Dermatol 1969;100:12-16.
3.Wilkie JS, Yager JA, Eyre P, et al. Morphometric analyses of the skin of dogs with atopic dermatitis and correlations with cutaneous and plasma histamine and total serum IgE. Vet Pathol 1990;27:179-186.
4.Hill PB, Moriello KA, DeBoer DJ. Concentrations of total serum IgE, IgA, and IgG in atopic and parasitized dogs. Vet Immunol Immunopathol 1995;44:105-113.
5.Hill PB, DeBoer DJ. Quantification of serum total IgE concentration in dogs by use of an enzyme-linked immunosorbent assay containing monoclonal murine anti-canine IgE. Am J Vet Res 1994;55:944-948.
6.Schwartzman RM. Immunologic studies of progeny of atopic dogs. Am J Vet Res 1984;45:375-378.
7.Halliwell RE, Kunkle GA. The radioallergosorbent test in the diagnosis of canine atopic disease. J Allergy Clin Immunol 1978;62:236-242.
8.Racine BP, Marti E, Busato A, et al. Influence of sex and age on serum total immunoglobulin E concentration in Beagles. Am J Vet Res 1999;60:93-97.
9.Griot-Wenk ME, Busato A, Welle M, et al. Total serum IgE and IgA antibody levels in healthy dogs of different breeds and exposed to different environments. Res Vet Sci 1999;67:239-243.
10.Deboer DJ, Hill PB. Serum immunoglobulin E in West Highland White Terrier puppies do not predict development of atopic dermatitis. Vet Dermatol 1999;10:275-281.
11.Fraser MA, McNeil PE, Gettinby G. Studies of serum total immunoglobulin E concentrations in atopic and non-atopic dogs. Vet Rec 2003;152:159-163.
12.Olivry T, Guaguère E, Héripret D. Treatment of canine atopic dermatitis with misoprostol, a prostaglandin E1 analogue: an open study. J Dermatol Treatment 1997;8:243-247.
13.Willemse A. Atopic skin disease : a review and a reconsideration of diagnostic criteria. J Small Anim Prac 1986;27:771-778.
14.Villar T, Holgates T. IgE, smoking and lung function. Clin Exp Allergy 1994;24:508-510.
15.Omenaas E, Bakke P, Elsayed S, et al. Total and specific serum IgE levels in adults: relationship to sex, age and environmental factors. Clin Exp Allergy 1994;24:530-539.
16.Zunic M. Comparison between IMMUNODOT tests and the intradermal skin test in atopic dogs. Vet Dermatol 1998;9:201-205.
17.Tarpataki N, Bigler B, Vajdovich P, et al. Comparison between an intradermal skin test and allergen-specific IgE-ELISA for canine atopic dermatitis. Schweiz Arch Tierheilkd 2008;150:117-122.
18.Park S, Ohya F, Yamashita K, et al. Comparison of response to immunotherapy by intradermal skin test and antigen-specific IgE in canine atopy. J Vet Med Sci 2000;62:983-988.
19.Mueller RS, Burrows A, Tsohalis J. Comparison of intradermal testing and serum testing for allergen-specific IgE using monoclonal IgE antibodies in 84 atopic dogs. Aust Vet J 1999;77:290-294.
20.Hämmerling R, De Weck AL. Comparison of two diagnostic tests for canine atopy using monoclonal anti-IgE antibodies. Vet Dermatol 1998;9:191-199.
21.Foster AP, Littlewood JD, Webb P, et al. Comparison of intradermal and serum testing for allergen-specific IgE using a Fcepsilon RIalpha-based assay in atopic dogs in the UK. Vet Immunol Immunopathol 2003;93:51-60.
22.DeBoer DJ, Hillier A. The ACVD task force on canine atopic dermatitis (XVI): laboratory evaluation of dogs with atopic dermatitis with serum-based "allergy" tests. Vet Immunol Immunopathol 2001;81:277-287.
23.Saevik BK, Ulstein TL, Larsen HJ. Evaluation of a commercially available enzyme-linked immunosorbent assay for the detection of allergen-specific IgE antibodies in dogs. Res Vet Sci 2003;74:37-45.
24.Muller GH, Kirk RW, Scott DW, et al. Muller & Kirk''s small animal dermatology. 6th ed. Philadelphia: W.B. Saunders, 2001.
25.Saridomichelakis MN, Koutinas AF, Gioulekas D, et al. Canine atopic dermatitis in Greece: clinical observations and the prevalence of positive intradermal test reactions in 91 spontaneous cases. Vet Immunol Immunopathol 1999;69:61-73.
26.Prèlaud P, Guaguére E, Alhaidari Z, et al. Reevaluation of diagnostic criteria of canine atopic dermatitis. Rev Med Vet 1998;149:1057-1064.
27.Lee KW, Blankenship KD, McCurry ZM, Esch RE, DeBoer DJ, Marsella R. Performance characteristics of a monoclonal antibody cocktail-based ELISA for detection of allergen-specific IgE in dogs and comparison with a high affinity IgE receptor-based ELISA. Vet Dermatol 2009;20:157-164.
28.Olivry T, Jackson HA, Murphy KM, Tater KC, Roberts M. Evaluation of a point-of-care immunodot assay for predicting results of allergen-specific intradermal and immunoglobulin E serological tests. Vet Dermatol 2005;16:117-120.
29.Saevik BK, Ulstein TL, Larsen HJ. Evaluation of a commercially available enzyme-linked immunosorbent assay for the detection of allergen-specific IgE antibodies in dogs. Res Vet Sci 2003;74:37-45.
30.Mari A, Iacovacci P, Afferni C, et al. Specific IgE to cross-reactive carbohydrate determinants strongly affect the in vitro diagnosis of allergic diseases. J Allergy Clin Immunol 1999;103:1005-1011.
31.Meyer CH, Bond JF, Chen MS, Kasaian MT. Comparison of the levels of the major allergens Der p I and Der p II in standardized extracts of the house dust mite, Dermatophagoides pteronyssinus. Clin Exp Allergy 1994;24:1041-1048.
32.Patel M, Selinger D, Mark GE, Hickey GJ, Hollis GF. Sequence of the dog immunoglobulin alpha and epsilon constant region genes. Immunogenetics 1995;41:282-286.
33.Stedman K, Lee K, Hunter S, Rivoire B, McCall C, Wassom D. Measurement of canine IgE using the alpha chain of the human high affinity IgE receptor. Vet Immunol Immunopathol 2001;78:349-355.
Chapter3
References
1.Bond R, Collin NS, Lloyd DH. Use of contact plates for the quantitative coutures of Malassezia pathydermatis from canine skin. J Small Anim Prac 1994;35:68-72.
2.Kennis RA, Rosser EJ, Jr., Olivier NB, et al. Quantity and distribution of Malassezia organisms on the skin of clinically normal dogs. J Am Vet Med Assoc 1996;208:1048-1051.
3.Bond R, Ferguson EA, Curtis CF, et al. Factors associated with elevated cutaneous Malassezia pachydermatis populations in dogs with pruritic skin disease. J Small Anim Pract 1996;37:103-107.
4.Morris DO, Olivier NB, Rosser EJ. Type-1 hypersensitivity reactions to Malassezia pachydermatis extracts in atopic dogs. Am J Vet Res 1998;59:836-841.
5.Chen TA, Halliwell RE, Pemberton AD, et al. Identification of major allergens of Malassezia pachydermatis in dogs with atopic dermatitis and Malassezia overgrowth. Vet Dermatol 2002;13:141-150.
6.Saridomichelakis MN, Farmaki R, Leontides LS, et al. Aetiology of canine otitis externa: a retrospective study of 100 cases. Vet Dermatol 2007;18:341-347.
7.Nardoni S, Dini M, Taccini F, et al. Occurrence, distribution and population size of Malassezia pachydermatis on skin and mucosae of atopic dogs. Vet Microbiol 2007;122:172-177.
8.Cafarchia C, Romito D, Sasanelli M, et al. The epidemiology of canine and feline dermatophytoses in southern Italy. Mycoses 2004;47:508-513.
9.Copetti MV, Santurio JM, Cavalheiro AS, et al. Dermatophytes isolated from dogs and cats suspected of dermatophytosis in Southern Brazil. Acta sci vet 2006;34:119-124.
10.Brilhante RS, Cavalcante CS, Soares-Junior FA, et al. High rate of Microsporum canis feline and canine dermatophytoses in Northeast Brazil: epidemiological and diagnostic features. Mycopathologia 2003;156:303-308.
11.Faergemann J. Atopic dermatitis and fungi. Clin Microbiol Rev 2002;15:545-563.
12.Rajka G, Barlinn C. On the significance of the trichophytin reactivity in atopic dermatitis. Acta Derm Venereol 1979;59:45-47.
13.Muller GH, Kirk RW, Scott DW, et al. Muller & Kirk''s small animal dermatology. 6th ed. Philadelphia: W.B. Saunders, 2001.
14.Cafarchia C, Gallo S, Romito D, et al. Frequency, body distribution, and population size of Malassezia species in healthy dogs and in dogs with localized cutaneous lesions. J Vet Diagn Invest 2005;17:316-322.
15.Prado MR, Brilhante RS, Cordeiro RA, et al. Frequency of yeasts and dermatophytes from healthy and diseased dogs. J Vet Diagn Invest 2008;20:197-202.
16.Cabanes FJ, Abarca ML, Bragulat MR. Dermatophytes isolated from domestic animals in Barcelona, Spain. Mycopathologia 1997;137:107-113.
17.Aljabre SH, Richardson MD, Scott EM, et al. Germination of Trichophyton mentagrophytes on human stratum corneum in vitro. J Med Vet Mycol 1992;30:145-152.
18.Aljabre SH, Richardson MD, Scott EM, et al. Adherence of arthroconidia and germlings of anthropophilic and zoophilic varieties of Trichophyton mentagrophytes to human corneocytes as an early event in the pathogenesis of dermatophytosis. Clin Exp Dermatol 1993;18:231-235.
19.Willemse A. Atopic skin disease : a review and a reconsideration of diagnostic criteria. J Small Anim Prac 1986;27:771-778.
20.Akerstedt J, Vollset I. Malassezia pachydermatis with special reference to canine skin disease. Br Vet J 1996;152:269-281.
21.Masuda A, Sukegawa T, Tani H, et al. Attachment of Malassezia pachydermatis to the ear dermal cells in canine otitis externa. J Vet Med Sci 2001;63:667-669.
22.Guzman-Chavez RE, Segundo-Zaragoza C, Cervantes-Olivares RA, Tapia-Perez G. Presence of keratinophilic fungi with special reference to dermatophytes on the haircoat of dogs and cats in Mexico and Nezahualcoyotl cities. Rev Latinoam Microbiol 2000;42:41-44.
23.Sparkes AH, Gruffydd-Jones TJ, Shaw SE, Wright AI, Stokes CR. Epidemiological and diagnostic features of canine and feline dermatophytosis in the United Kingdom from 1956 to 1991. Vet Rec 1993;133:57-61.
24.Mancianti F, Nardoni S, Cecchi S, Corazza M, Taccini F. Dermatophytes isolated from symptomatic dogs and cats in Tuscany, Italy during a 15-year-period. Mycopathologia 2002;156:13-18.
25.Silva V, Thomson P, Maier L, Anticevic S. [Dermatophyte infection and colonization in dogs from South Santiago, Chile]. Rev Iberoam Micol 2003;20:145-148.
26.Saridomichelakis MN, Koutinas AF, Gioulekas D, Leontidis L. Canine atopic dermatitis in Greece: clinical observations and the prevalence of positive intradermal test reactions in 91 spontaneous cases. Vet Immunol Immunopathol 1999;69:61-73.
27.Colombo S, Hill PB, Shaw DJ, Thoday KL. Requirement for additional treatment for dogs with atopic dermatitis undergoing allergen-specific immunotherapy. Vet Rec 2007;160:861-864.