臺灣博碩士論文加值系統

電紡技術近來也常應用於生醫工程領域，其製成的奈米纖維膜具有孔洞，微小的孔洞既可讓養份及廢棄物通透也可防止組織向內生長可有效的形成屏障，因此本研究運用聚己內酯(polycaprolactone)良好的生物相容性及可降解特性，加入聚乙二醇(polyethylene glycol)來增強延展性和親水性，以電紡絲法製得聚己內酯/聚乙二醇( PECL)奈米纖維膜，同時在裡面加入Amoxicillin與Ibuprofen兩種藥物，來進一步評估其促進肌腱癒合及防術後肌腱沾黏(adhesion)形成的效果，並分析其物性並評估纖維母細胞(rabbit dermal fibroblast)在其奈米纖維膜上之細胞貼覆、生長及存活等生物相容性測試，及動物實驗分析。綜合分析結果得知，Ibuprofen在纖維裡為均勻混合相，也觀察到裡面無結晶體；而在動物實驗中發現，含有藥物的奈米纖維膜確實對於抗沾黏預防，有一定程度的效果。因此含有藥物的奈米纖維膜對於抗沾黏極具潛在性。

Electro-spinning technology, has also often used in the field of biomedical engineering, nano fiber membrane made of it has holes, the tiny holes can allow nutrients and waste pass through and prevents tissue ingrowth that effectively form a barrier, Therefore, this study uses polycaprolactone, it has good biocompatibility and biodegradability properties, polyethylene glycol is added to enhance the ductility and hydrophilicity, produce polycaprolactone/ polyethylene glycol (PECL) nano fiber membrane, and add two drugs, Amoxicillin and Ibuprofen, to evaluate its promotion tendon healing and anti-adhesion formation after effects, and analyze their properties and assess fibroblasts (rabbit dermal fibroblast) in its nano fiber membrane, such as growth, cell cover, survival of biocompatibility testing, and animal experimental analysis. Comprehensive analysis of the results of that, Ibuprofen in the fiber is uniformly mixed phase, which is also observed there is no crystals in it; while in animal experiments, the drug-containing nano fiber membrane is indeed the anti-adhesion prevention, there is a certain degree of effectiveness. Therefore, the drug-containing nano fiber membrane has potential for anti-adhesion great.

總目錄
指導教授推薦書
口試委員會審定書
長庚大學博碩士論文著作授權書 iii
致謝 iv
中文摘要 v
Abstract vi
總目錄 vii
圖目錄 xi
表目錄 xiii
第一章導論 1
1.1前言 1
1.2 研究動機與目的 1
第二章原理及文獻回顧 3
2.1 沾黏 3
2.1.1沾黏的定義 3
2.1.2手術後沾黏所引起之併發症 3
2.1.3 沾黏形成的病理 3
2.1.4 肌腱與腹膜修復的差異 5
2.1.5 沾黏之預防 7
2.2 靜電紡絲技術 12
2.2.1 靜電紡絲發展起源 12
2.2.2 靜電紡絲原理 13
2.2.3 靜電紡絲設備 13
2.2.4 影響靜電紡絲因素 14
2.3生醫材料 21
2.3.1 聚己內酯 22
2.3.2 聚乙二醇 23
2.4 Amoxicillin 24
2.5 Ibuprofen 25
第三章實驗材料、設備與方法 27
3.1實驗用藥品與設備 27
3.1.1 化學藥品試劑 27
3.1.2 商業試劑套組 27
3.1.3 細胞培養材料及藥品 27
3.1.4 實驗儀器 28
3.2 實驗方法與步驟 30
3.2.1 製備含藥物/PECL電紡溶液 30
3.2.2 奈米纖維薄膜製備方式 30
3.2.3 奈米纖維薄膜之物性分析 31
3.2.3.1 掃描式電子顯微鏡纖維型態分析 (SEM) 31
3.2.3.2 傅立葉轉紅外線光譜儀分析 (FT-IR) 31
3.2.3.3 X光結晶繞射分析 (XRD) 32
3.2.3.4 熱重分析儀分析 (TGA) 32
3.2.3.5 親疏水性分析 32
3.2.3.6 機械強度測試 33
3.2.3.7 示差掃描熱量分析儀(DSC) 33
3.2.4藥物釋放分析 33
3.2.5老鼠纖維母細胞(NIH-3T3)繼代培養 34
3.2.6老鼠纖維母細胞培養在奈米纖維薄膜的處理步驟 34
3.2.7 MTS細胞活性分析 35
3.2.8動物實驗 35
第四章結果與討論 37
4.1探討不同藥物對奈米纖維的影響 37
4.2探討奈米纖維薄膜的親疏水性 41
4.3探討奈米纖維薄膜熱重分析結果 (TGA) 43
4.4 探討奈米纖維薄膜示差掃描熱量分析儀結果 (DSC) 45
4.5 探討奈米纖維薄膜X光結晶繞射儀結果 (XRD) 46
4.6 機械強度結果分析 47
4.7傅立葉轉紅外線光譜儀分析結果 (FT-IR) 49
4.8 藥物釋放結果分析 50
4.9 細胞貼附測試 51
4.10 動物實驗結果分析 52
第五章結論 53
第六章參考文獻 54


圖目錄
圖2.1腹膜修復過程中所參與之各種細胞組成 …………………... .5
圖2.2肌腱修復之流程圖…………………………………………. …6
圖2.3電紡設備的示意圖…………………………………………….14
圖2.4聚己內酯之結構圖………………………………………. . .….23
圖2.5聚乙二醇之結構圖…………………………………. ……. .….24
圖2.6 Amoxicillin之結構圖………………………………. . ……….25
圖2.7 Ibuprofen之結構圖………………………………. . …. .…….26
圖4.1探討不同藥物對奈米纖維膜的影響……………………….…38
圖4.2奈米纖維膜的直徑分佈圖………………. ………….………..39
圖4.3水接觸角之示意圖 ………………………. ………….………42
圖4.4 TGA重量損失分析圖…………………………………………44
圖4.5 TGA重量損失微分分析圖…………….. …………………… 44
圖4.6示差掃描熱量分析儀結果 ………………….. ……………….45
圖4.7 XRD分析圖 ………………………………...…..…..………..46
圖4.8拉伸應力-應變曲線圖………………………………………...48
圖4.9傅立葉轉紅外線光譜儀分析結果 ……………………………49
圖4.10藥物釋放分析 ……………………………………………….50
圖4.11 細胞貼附分析圖(N=3)……………………………..………..51
圖4.12動物實驗肌腱沾黏情形…………………..…………..………52
表目錄
表2.1隔離膜的條件……………………………………………….... . .8
表4.1電紡纖維膜之楊氏係數和應變-應力(N=3)…………………. .48

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