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研究生:楊雨涵
研究生(外文):Yu-Han Yang
論文名稱:熱處理對柑桔類及香蕉品質之影響
論文名稱(外文):Effects of Heat Treatments on Quality of Citrus and
指導教授:洪登村洪登村引用關係謝慶昌謝慶昌引用關係
指導教授(外文):Deng-Tsen HorngChing-Chang Shiesh
學位類別:碩士
校院名稱:國立中興大學
系所名稱:園藝學系
學門:農業科學學門
學類:園藝學類
論文種類:學術論文
論文出版年:2003
畢業學年度:91
語文別:中文
論文頁數:97
中文關鍵詞:熱處理柑桔香蕉綠黴病生理斑點
外文關鍵詞:heat treatmentcitrus fruitsbananagreen moldsenescent spots
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本試驗首先探討熱風處理對椪柑腐爛的影響,椪柑果實經接種綠黴菌孢子後以40℃熱風處理1日以上或以35℃熱風處理2日以上皆可有效控制腐爛的發生;若在椪柑果實在熱風處理後進行青黴菌孢子接種,果實的腐爛並沒有因熱處理而減少,顯示台灣產之椪柑,在熱風處理下,對腐爛的抑制效果,可能是直接對病原菌熱致死的作用,而非經由熱處理以提升果實抗菌能力的途徑。
另外,本試驗亦調查部分柑桔類在熱風處理下,其糖度、可滴定酸及糖酸比之變化。在明尼桔柚、豔陽柑、柳橙、臍橙、檸檬及葡萄柚等種類,在熱風處理下,可滴定酸皆有減少的現象,以40℃處理3日者為例,明尼桔柚減少10%、豔陽柑減少21%、柳橙減少27%、臍橙減少17%、檸檬減少9%及葡萄柚減少10%、而熱風處理對糖度的影響較小,故上述品種皆可因熱風處理而提升其果汁之糖酸比。但椪柑果實在熱風處理中可滴定酸雖有下降的趨勢,但效果不明顯。可滴定酸的下降可能由於熱風處理期間呼吸率大幅上升,而有機酸作為呼吸基質所造成。
柳橙以35℃熱風處理2天後,於25℃下通風貯藏30天後,結果顯示:經熱風處理者其綠黴菌引起的腐爛確有減少的趨勢,但蒂腐病卻比對照組者高,所以在總腐爛率方面,則熱風處理與對照組一樣。
本試驗之目的在於探討香蕉溫湯處理後抑制生理斑點發生之機制,‘北蕉’香蕉在53℃溫湯處理5分鐘,可明顯抑制生理斑點之發生。由於溫湯處理對於整個果實乙烯釋放率並沒有促進的效果,反而會降低果皮乙烯釋放率且降低果實組成間乙烯濃度,所以溫湯處理可能不是因促進乙烯大量生合成而由乙烯抑制生理斑點之發生。生理斑點的出現是一種果皮褐化現象,而和褐化有關的酵素如多酚氧化酵素及其基質酚類化合物,皆不因熱處理而有明顯的變化,故溫湯處理並非由抑制多酚氧化酵素或降低酚類化合物含量以減少褐化現象,但另一種酵素─過氧化酵素活性可因熱處理而下降,所以其褐化上所扮演的角色,值得進一步探討。由離子滲漏率、果實組織內氣體濃度及直接由細微構造觀察,果皮在溫湯處理後其對物質的通透性明顯增加,可能因此使果肉形成促成生理斑點發生的物質不易在果皮上累積,而抑制生理斑點的發生。
First, we explored the effects of hot-air treatments on the spoilage of ‘Ponkan’ fruits. The results showed that the effective control of spoilage could be affected by subjecting the green mold spore inoculated fruits either to 40℃ hot air for more than one day or to 35℃ hot air for more than two days. No favorable results could be obtained when blue mold spore inoculated fruits were treated under the same conditions. The data indicated that heat treatments killed the spores of the mold rather than improve the resistant capability of the fruits.
In addition, we studied the impacts of hot-air treatments on the sugar contents, titratable acids and sugar/acids of some of Citrus spp. fruits. Hot-air treatments were found ,with the exception of ‘Ponkan’ mandrin, to diminish the amount of titratable acids of ‘Minneola’ tangelo, ‘Sunburst’ tangelo, ‘Liu Cheng’ orange, ‘Navel’ orange, lemon and ‘Ruby’ grapefruit. Taking the 40℃, 3-days experiment for example, the titratable acids in ‘Minneola’ tangelo, ‘Sunburst’ tangelo, ‘Liu Cheng’ orange, ‘Navel’ orange, ‘Eureka’ Lemon and ‘Ruby’ grapefruit were lowered by 10%, 21%, 27%, 17%, 9% and 10% respectively. However, similar treatments only caused minimal changes in the sugar contents of these fruits. The sugar to acid ratio in the juices of the above said cultivars, therefore, could be upgraded simply by heat treatments. The drop of titratable acids might be the result of drastic elevation of their respiration rate, which consumed the acids as substrates in the process.
The orange that was subjected to two-days, 35℃, hot-air treatments followed by storing at 25℃ for 30 days in an air room tended to be better off than that of the controls in terms of the damage caused by the green mold. But, on the other hand, the risk of getting stem-end rot was quite high. In general, as far as the total spoilage rate is concern, there is no much difference between the hot air treated sets and the controls.
The objectives of this experiment were to investigate the mechanism of actions of inhibition of senescent spots of banana after hot water (53℃) treatment.
Banana after treatment for 5 minutes inhibited the development of senescent spots significantly. However the treatment failed to stimulate but reduced the release of ethylene from the banana fruits. Thus it seemed unlikely that the 53℃ water treatment could enhance ethylene biosynthesis or lead to the inhibition of the spotting problem.
Spotting is a physiological disorder of peel browning during ripening. Some of the enzymes related to the browning phenomenon such as polyphenol oxidase whose activity was not affected by the water treatment seemed to rule out the possibilities of involving in the browning process. But one other browning-related enzyme, peroxidase, which was reduced in activity by the warm water treatment served to show the likelihood of involving in the development of spotting and its role in the browning merit further investigation.
According to the experiments of ion leakage, gas concentration in the fruit tissues and unltrastructure of fruits, the permeability of fruit peel was significantly increased after the water treatment. This may reduce the possibility of accumulation of brown pigments in the banana peel and prevent the senescent spots from occurring.
目錄
頁次
序言…………………………………………….…….. 1
第一單元 前人研究…………………………….…… 2
一.熱處理之方法…………………………………………. 2
二.熱處理抑制果實採收後之腐爛………………………. 3
三.熱處理對果實寒害之影響……………………………. 5
四.熱處理對果實後熟及品質之影響……………………. 6
第二單元 熱風處理對柑桔類品質之影響…….…… 9
中文摘要………………………………………………….. 9
英文摘要………………………………………………….. 10
壹.前言……………………………………………………. 11
貳.前人研究………………………………………………. 12
參.材料與方法……………………………………………. 20
肆.結果……………………………………………………. 25
伍.討論……………………………………………………. 31
第三單元 溫湯處理對香蕉生理斑點之影響….…… 50
中文摘要………………………………………………….. 50
英文摘要………………………………………………….. 51
壹.前言……………………………………………………. 52
貳.前人研究………………………………………………. 53
參.材料與方法……………………………………………. 60
肆.結果……………………………………………………. 65
伍.討論……………………………………………………. 70
參考文獻……………………………………….…….. 87
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