臺灣博碩士論文加值系統

本論文係針對考慮質體損失機率為可變且微生物對環境的改變會有調適的時間延遲，而進料受質濃度呈週期性改變的連續式攪拌槽反應器加以分析。由數值模擬所得的結果繪製出在稀釋速率D對週期P之平面上的操作圖，以顯示出兩種微生物最後仍會共存的操作條件。結果顯示當含質體與不含質體微生物之延遲時間相差足夠大時，這兩種微生物最後就有可能共存，且延遲時間差異愈大，呈現在D-P平面上之共存區也愈大；最大之進料受質濃度越高，共存區也愈大；在一週期中開與關的時間比太大或太小都會導致含質體微生物被沖盡，而該比值有一能得最大共存區的最佳值。文中並討論兩種微生物之最大比生長速率之比值對共存區的影響，以及在兩種微生物共存時含質體微生物所佔的質量分率受操作參數週期、稀釋速率、最大進料受質濃度和開與關之時間比的影響。

The continuous stirred tank reactor (CSTR) with cycling in the feed substrate concentration has been analyzed by numerical simulations for the case where the plasmid loss probability is variable and a difference in times for the plasmid-bearing and plasmid-free microorganisms to adapt to changing environment exists. Operating diagrams on the D (dilution rate) — P (period) plane are constructed to show the operating conditions under which these two microorganisms will ultimately coexist. It is found that both microorganisms can coexist ultimately if there is sufficient difference in the adaptation times, and the larger the difference is, the larger the coexistance region will be ; larger coexistance regions are also obtained for larger maximum feed substrate concentrations. Washout of the plasmid-bearing microorganisms occurs for the case where the on-off time ratio is either too larger or too small, and there exists an optimal ratio which yields the largest coexistance regions. The effect of the ratio of maximum specific growth rates on the coexistence region, and the effects of the operating parameters period, dilution rate, maximum feed substrate concentration and on-off time ratio on the ultimate mass fraction of the plasmid-bearing microorganisms are also discussed.

中文摘要…………………………………………………………vi
英文摘要………………………………………………………vii
目錄…………………………………………………………viii
圖目錄…………………………………………………………ix
表目錄…………………………………………………………xiv
第一章 緒論……………………………………………………1
第二章 系統方程式之推導及數值解…………………………7
2.1 系統之動態方程式……………………………………7
2.2 方程式之數值解……………………………………15
2.3 系統模擬與結果分析流程圖………………………22
第三章 結果與討論…………………………………………23
3.1開關時間比為1之情況…………………………….…25
3.1.1最大進料受質濃度對共存區之影響………………25
3.1.2延遲時間之比值對共存區之影響…………………25
3.1.3最大比生長速率之比值對共存區之影響…………27
3.1.4質體損失機率(定值)對共存區之影響…….………27
3.1.5操作參數Smax、D及P對R值的影響……………32
3.2開關時間比不為1之情況…………………………49
3.2.1開關時間比對共存區之影響…………….…………49
3.2.2操作參數Smax、D、P及開關時間比對R值的影 響…………………………………...………………51
3.3兩種微生物共存的參數範圍…………………………64
第四章 結論…………………………………………………66
符號說明………………………………………………………68
參考文獻………………………………………………………71
附錄…………………………………………………………75

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