臺灣博碩士論文加值系統

β-sitosterol、stigmasterol為一個已被證實具有療效的成份，可控制膽固醇、抗癌、促進攝護腺健康、增強免疫力等等。
民間常有用香蕉花食療，香蕉亦為台灣常見之植物，探討香蕉花中有否β-sitosterol、stigmasterol，研究其β-sitosterol、stigmasterol的含量是多少，從而可以將香蕉花開發為β-sitosterol、stigmasterol的來源，可提升香蕉花應用價值。
本研究所採用的研究對象為香蕉花，確定其含有β-sitosterol、stigmasterol之後，香蕉花中β-sitosterol、stigmasterol定量分析是一個具有研究價值的課題。而實驗方法主要藉由高效能液相層析搭配光二級陣列偵測器(HPLC/DAD)分析香蕉花中β-sitosterol、stigmasterol成份，此乃利用高效能液相層析對香蕉花中β-sitosterol、stigmasterol找尋適當的分析條件，再進一步去對β-sitosterol、stigmasterol做定量分析。
分析β-sitosterol、stigmasterol ，偵測器波長設在UV 205nm，移動相以甲醇100%可快速分析此兩種重要之植物固醇 β-sitosterol、stigmasterol。
依上述定量分析之條件，再以平均定量概念，取其多次實驗結果平均數值，為定量結果。香蕉花1g中 β–sitosterol 含量 384.00±8.10（µg/g）、stigmasterol含量 281.00±6.60（µg/g）
隨著現代人生活水準的不斷提高，保健意識的不斷增強，對健康大有裨益的各種功能保健食品越來越受到人們青睞，市場需求日益增加。因而有機會開發香蕉花為以β-sitosterol、stigmasterol成分為標榜的保健食品。

目 錄

中文摘要………………………………………………………. i
英文摘要……………………………………………………....iii
目錄…………………………………………………………….iv
表目錄…………………………………………………………vii
圖目錄………………………………………………………...viii


第一章 緒論……………………………………………………………1
一、前言……………………………………………………………….1
二、研究動機………………………………………………………….2

第二章 背景資料與文獻回顧…………………………………………4
一、香蕉植物之介紹…………………………………………………4
(一)香蕉之植物學分類………………………………………….4
(二)香蕉植物形態特徵………………………………………….5
(三)香蕉開花與繁殖...................................... .......... ...................7
二、β-sitosterol、stigmasterol介紹.......... .......................................8
(一) β-sitosterol、stigmasterol的結構.........................................8
(二) β-sitosterol、stigmasterol的來源…………………………11
(三) β-sitosterol、stigmasterol的生理功效……………………11
(四)植物固醇成分的萃取溶劑及條件………………………...14
(五)植物固醇分析方法………………………………………...14

第三章 實驗材料及儀器……………………………………………..16
一、植物採集………………………………………………………..16
二、藥品溶劑………………………………………………………..16
三、儀器設備………………………………………………………..16
四、實驗使用之高效液相層析儀器……………..…………………17

第四章 實驗方法……………………………………………………..20
一、藥品配製部份…………………………………………………..20
二、製作β-sitosterol、stigmasterol之檢量線，求線性方程式、線性 相關係數以及偵測極限………………………………………..21
三、儀器參數設定以及分析β-sitosterol、stigmasterol基本條件….22
四、香蕉花之樣品前處理…………………………………………..22
五、移動相與β-sitosterol、stigmasterol保留時間關係探討………25
六、β-sitosterol、stigmasterol之標準添加法………………………26
七、針對β-sitosterol、stigmasterol精密度之測定………………….28
八、針對β-sitosterol、stigmasterol回收率之測定………………….28

第五章 結果與討論…………………………………………………..30
一、移動相的探討…………………………………………………..30
二、移動相與β-sitosterol、stigmasterol保留時間關係探討………34
三、香蕉花樣品前處理實驗探討…………………………………..37
四、β-sitosterol、stigmasterol之檢量線，線性方程式、線性相關係數以及偵測極限……………………………………..................42
五、β-sitosterol、stigmasterol標準添加法實驗結果………………46
六、β-sitosterol、stigmasterol精密度之測定結果…………………48
七、回收率之測定結果……………………………………………..49
八、探討真實樣品層析圖中β-sitosterol、stigmasterol……………53
九、香蕉花真實樣品中β-sitosterol、stigmasterol定量分析………65
十、結論……………………………………………………………..67

第六章 參考文獻……………………………………………………..68

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