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ABO blood group is clinically the most important blood group system in transfusion medicine including fours common different phenotypes . It has been a valuable tool in transfusion medicine , physical anthropology , disputed parentage testing , human identification , and in forensic analysis . Historically , forensic and clinical labatories utilize serological techniques to identify ABO blood type . The testing of forensic samples for ABO types using serological techniques has several drawbacks . We has simplified the identification of ABO types by taking advantage ofpreviouly reported ABO DNA sequence difference . The use of single strand conformation polymorphism ( SSCP ) can separate sequence polymorphism than differ by only one base . We has investigated in 260 Chinese donors by multiplexed Polymerase Chain Reaction . In this study the ABO alleles from exon 6 , producing a 212/213 bp fragment , and exon 7, which produces a fragment of 272 bp, were separated by non-denaturing polyacrylamide gels. The two exons were amplified in a single reaction that produces similar quantities of DNA for both exons . However , in 260 SSCP patterens we can clustered 9 into different distinct and discernible groups . A concordance rate of 98.5 % (256/260 samples) observed between the actural genotype and the serologically-based predicted genotype . These results indicate that the assay provides a rapid , accurate , and simple method for ABO genotyping that serves as a useful supplement to standard serological ABO typing.
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