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研究生:許媛媛
研究生(外文):Yuan-yuan Hsu
論文名稱:高身小鰾鮈親緣地理之研究
論文名稱(外文):Phylogeography of Microphysogobio alticorpus
指導教授:曾登裕曾登裕引用關係
指導教授(外文):Deng-Yu Tseng
學位類別:碩士
校院名稱:國立臺南大學
系所名稱:生態科學與技術學系環境生態碩士班
學門:環境保護學門
學類:環境資源學類
論文種類:學術論文
論文出版年:2013
畢業學年度:101
語文別:中文
論文頁數:80
中文關鍵詞:控制區(D-Loop)細胞色素b高身小鰾鮈親緣地理生物學
外文關鍵詞:D-loopcytochrome bMicrophysogobio alticorpusPhylogeography
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本研究利用粒線體細胞色素b (cytochrome b)與控制區(D-loop region)基因序列來探討台灣地區淡水魚類親緣地理模式,採六個台灣河系的高身小鰾鮈樣本來進行親緣地理生物學分析的相關研究。90個樣本的粒線體DNA 控制區(D-Loop) 基因型共有12種單倍型,粒線體DNA細胞色素b基因型共有17種單倍型。透過親緣圖分析,出現三個親緣單倍型,族群分化結果顯示出現顯著的遺傳結構(FST = 0.78和0.89)。根據Mismatch distribution analysis、neutrality tests 及Bayesian skyline plots等測驗分析顯示高身小鰾鮈雖然各河系具有高度分化,卻沒有顯著的族群擴張,本研究認為此和水體與族群大小、
穩定度有關係。
In this study, make ues of the mitchondrial DNA cytochrome b and D-loop gene to explore Taiwan freshwater fish phylogeographic pattern and phylogeographical analyses on
Microphysogobio alticorpus samples from six rivers in Taiwan were conducted for biogeographic studies. A total of 12 haplotypes were genotyped for mtDNA D-loop gene
and 17 haplotypes were genotyped for mtDNA cytochrome b gene in 90 specimens. Three major phylogenetic haplotype groups were revealed via phylogenetic analysis. Population
differentiation analyses indicated significant genetic structure (FST = 0.78 and 0.89).Mismatch distribution analysis, neutrality tests and Bayesian skyline plots indicated the river is highly differentiated , but no significant population expansion for Microphysogobio
alticorpus. This study suggests that the size and stability of population ,and the water body size as the major reason.
第一章 前言...............................................1
一、研究背景理論..........................................1
(一)親緣地理學(Phylogeography)............................1
(二)溯祖理論(Coalescent theory) ..........................2
(三)地質事件對物種分布的影響..............................3
(四)網狀親緣圖之建立......................................4
(五) 分子技術於族群遺傳結構與親緣地理學之應用.............4
二、淡水魚類的型態和拓殖..................................6
三、台灣淡水魚的起源、地理分布............................7
四、研究物種-高身小鰾鮈...................................9
五、研究目的..............................................9
第二章 材料與方法........................................10
一、研究方法.............................................10
(一)採樣.................................................10
(二)粒線體DNA 萃取.......................................10
(三) DNA 引子(Primer)設計................................10
(四)聚合酶連鎖(PCR)反應..................................11
(五)電泳(Electrophoresis) ...............................11
(六)PCR 產物純化.........................................11
二、資料分析.............................................12
(一)定序比對和排序.......................................12
(二) 親緣地理關係親緣重建................................12
(三) 遺傳多樣性及遺傳結構................................13
(四) 族群變動分析........................................13
第三章 結果..............................................15
一、遺傳變異分析(Genetic Diversity) .....................15
二、親緣關係之重建(Phylogeny Reconstruction) ............16
三、網狀親緣關係及系群分析(Network estimation and nested clade analysis) .........................................17
四、族群結構與分子變方分析(Population Structure and AMOVA analysis) ...............................................18
五、族群歷史動態分析(Historical demographic analysis)....20
第四章 討論..............................................23
參考文獻.................................................27
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