臺灣博碩士論文加值系統

板藍根的基原為十字花科菘藍Isatis indigotica FORTUNE的乾燥根，為一種清熱解毒、涼血利咽的中藥材，對於流感、腮腺炎、肝炎、喉痹咽腫等症狀皆有療效。SARS期間，板藍根是幾種台灣常用的藥材之一，由中醫理論可以得知SARS屬於溫疫的範疇，其表徵為高熱、惡寒、全身痠痛、咳嗽、喉嚨炎等症狀，癒後會衍生肺炎及肺纖維化。本研究利用清熱解毒的中藥材板藍根，以動物模型來評估板藍根對肺纖維化防治的效果。
本研究經由全自動生化分析儀以及分光光度計來分析投予不同劑量的板藍根甲醇粗抽物下，經由bleomycin誘導肺部急性發炎及慢性纖維化期間，其肺臟組織內生化值的變化情形。實驗結果發現，在急性發炎期間，菘藍甲醇粗抽物對肺臟的抗氧化酵素穀胱苷肽還原酶(Glutathione reductase，GR)和超氧歧化酶(Superoxide dismutase，SOD)的活性有向上提升的作用並會減少肺臟組織中腫瘤壞死因子(Tumor Necrosis Factor-α，TNF-α)及肺臟組織中白介素-1β(Interleukin-1β，IL-1β)。經過動物模型誘導的肺臟發炎，持續的投予板藍根甲醇粗抽物能夠有效提升肺臟抗氧化酵素的活性，而且發現在高劑量(1.0 g/kg)下有顯著的效果。經由肺臟檢體的組織切片染色後，可以用半定量型態學分析來判斷肺臟的發炎情形；經過判讀後，結果和測定出來的生化值有相同的趨勢，在投予板藍根甲醇粗抽物後，減輕了肺臟的發炎程度。另一方面，經過七週持續投予板藍根甲醇粗抽物後，透過肺臟組織hydroxyproline的定量，膠原蛋白的測定，TGF-β的含量與纖維化組織特殊染色來了解板藍根對於纖維化防治的影響。測定結果顯示，高劑量(1.0 g/kg)的菘藍甲醇粗抽物能夠有效降低肺臟組織水解脯氨酸、膠原蛋白、TGF-β的濃度。
由此可知，高劑量(1.0 g/kg)的菘藍甲醇粗抽物能夠有效降低肺臟經過bleomycin的氧化傷害，更進ㄧ步降低纖維母細胞分泌的膠原蛋白含量，減低肺部纖維化的程度。

The root of Isatis indigotica FORTUNE (Cruciferae), popularly known as Ban-Lan-Gen, was used in traditional Chinese medicine for seasonal febrile diseases, mumps, hepatitis, inflammatory diseases, and sore throat.

During the SARS epidemic in 2003 in Taiwan, Ban-Lan-Gan was one of the most commonly used Chinese herb. In traditional Chinese medical science, SARS was one kind of pestilence with several severe syndromes, such as high fevers, chills, aches, coughs and faucitis. SARS was accounted for pneumonia and pulmonary fibrosis in the later stage. In this study, we evaluated the effect of Isatis indigotica radix extract in experimental murine model against pulmonary fibrosis.

In this study, the effects of different doses of methanol extracts of Isatis indigotica radix (II-RMeOH) on pulmonary fibrosis induced by intranasal inoculated bleomycin for 3 weeks in C57BL/6J mice were assayed by a spectrophotometer and a clinical chemistry analyzer. The results showed that II-RMeOH (1.0 g/ kg) caused significant increase in superoxide dismutase (SOD) and glutathione reducetase (GR) activeities; moreover, it also caused critical decrease in tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) level in mouse lung tissue.

Hematoxylin/ eosin staining indicated the inflammatory lung injury and the degree of inflammation by semiquantitative morphological index (SMI). On the other hand, after 7-week gavaging abidingly, the effect of II-RMeOH on the bleomycin-induced chronic pulmonary fibrosis was investigated. The study was evaluated by measuring the hydroxylproline, collagen, transforming growth factor-β1 (TGF-β1), and microwave Masson’s trichrome stain. The result showed that high-dose II-RMeOH (1.0 g/ kg) could significantly lower the contents of hydroxylproline, collagen, and TGF-β1 in lung tissue. Therefore, high-dose II-RMeOH (1.0 g/ kg) could reduce the bleomycin-induced lung inflammation. More over, it could diminish the collagen secretion by fibroblast and decrease the degree of pulmonary fibrosis.

目錄Ⅰ
略字表Ⅲ
圖目錄Ⅳ
摘要Ⅵ
英文摘要Ⅷ
第一章 緒 言1
第二章 總 論3
第一節 菘藍的文獻考察3
一、 板藍根的本草學考察3
二、 菘藍的藥用植物學考察10
三、 菘藍的成分文獻考察12
四、 菘藍的藥理作用文獻考察13
第二節 肺纖維化的形成16
一、 肺纖維化形成的機轉16
二、 氧化壓力和肺臟疾病19
三、 肺纖維化實驗動物模型和其原理21
第三節 自由基產生的氧化性損傷與發炎的關係23
ㄧ、發炎與過氧化的關聯性23
二、自由基的種類和特性25
三、抗氧化和自由基的關聯28
第三章 實驗材料與方法30
第一節 實驗材料30
一、 材料的製備30
二、 試藥30
三、 實驗儀器31
四、 實驗動物 32
第二節 實驗方法33
一、 抗發炎實驗模式33
二、 防治肺纖維化實驗模式42
第三節 統計分析45
第四章 實驗結果46
一、 肺纖維化前期抗發炎實驗46
二、 菘藍防治肺纖維化實驗64
第五章 討論69
第六章 結論75
參考文獻 81

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