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研究生:李鳳嬌
研究生(外文):Wanwisa Chumngoen
論文名稱:加熱處理對台灣黑羽土雞及白肉雞肉質特性影響之探討
論文名稱(外文):Investigation of the thermal treatments on quality attributes of black-feathered native and broiler chicken meat and interpretation of the relationship between parameters
指導教授:譚發瑞劉登城
指導教授(外文):Fa-Jui TanDeng-Cheng Liu
口試委員:黃振芳林國維陳志峰
口試委員(外文):Jeng-Feng HuangKuo-Wei LinChih-Feng Chen
口試日期:2016-07-07
學位類別:博士
校院名稱:國立中興大學
系所名稱:動物科學系所
學門:農業科學學門
學類:畜牧學類
論文種類:學術論文
論文出版年:2016
畢業學年度:104
語文別:英文
論文頁數:115
中文關鍵詞:白肉雞描述性感官品評加熱處理熟肉品質土雞物理化學性質
外文關鍵詞:BroilerDescriptive sensory analysisHeat treatmentMeat eating qualityNative chickenPhysicochemical characteristics
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雞肉廣泛地被大眾消費者所接受,消費量逐年上升。土雞肉具有特殊之風味及肉質特性,使得其感官特性與白肉雞有明顯差異。藉由分析台灣土雞 (Taiwan native chicken, TNC) 及商業白肉雞 (commercial broiler, BR) 之描述性感官品評 (descriptive sensory analysis) 及物化特性,並探討不同烹煮條件對於雞肉品質及適口性之影響,可以瞭解各雞種肉質特性,促進台灣土雞之消費,對於產業界具有關鍵性影響。因此本研究主要目的為探討不同加熱處理對於雞肉物化性質、描述性感官品評及組織結構等之影響,且分析各項品質地特性之關係。試驗一結果顯示, BR及TNC雞肉在多汁性、風味和質地具有顯著差異 (P < 0.05)。相較於BR組,TNC肉烹煮後有較低之蒸煮失重,使其於感官品評分析時,藉由咀嚼釋出較多水分,提高吞嚥後口中之滑順程度。TNC肉具有較高之質地感官品評特性,且雞肉風味較BR強烈,因此BR及TNC肉於質地感官品評特性上具有顯著之差異;肉中膠原蛋白含量、截切值及蒸煮失重等物性質地分析結果,亦與質地感官品評特性間具有高度相關 (r ≈ 0.7-0.9),皆顯著地影響雞肉品質。試驗二中,將TNC及BR之胸肉加熱至不同之中心溫度 (50-95°C) 後進行分析,隨著雞肉之中心溫度上升,兩組之蒸煮失重、可溶性膠原蛋白含量、截切值及硬度皆增加,其中雞肉之質地特性及可溶性蛋白質含量、蒸煮失重間具有高度相關性。試驗三則探討裝袋後烹煮 (in-bag cooking, BC) 及沸水直接烹煮 (cooked directly in hot water, WC) 兩種烹煮方法對雞胸肉之影響,結果顯示,在截切值、膠原蛋白含量、顯微結構、紅色度、黃色度及描述性感官品評上,兩者皆無顯著差異 (P > 0.05),但BC組別有顯著較高之亮度、烹煮失重及較低蛋白質溶解度 (P < 0.05)。試驗四中探討濕熱 (water-cooking, WC) 及乾熱 (oven-cooking, OC) 兩種烹煮方式對TNC胸肉之影響,結果顯示OC組有顯著較高之烹煮時間、蒸煮失重、截切值及較暗之顏色 (P < 0.05)。加熱後兩者之蛋白質溶解度皆會下降,但兩者間無顯著差異。加熱後WC組有顯著較高之膠原蛋白溶解度及肌原纖維破斷指數。OC組之肌肉纖維收縮程度顯著較高 (P < 0.05)。描述性感官品評結果顯示,WC組有較高之水份釋出及較低之質地特性;OC組則有較深之顏色及雞肉風味。綜觀上述,描述性感官品評可描述及量化雞肉之感官特性。此外,藉由儀器及感官品評測定結果相互對照,可得知感官特性及物理化學特性間具有高度相關性。由本研究結果得知,不同烹煮方式及條件對於雞肉熟肉品質及適口性有顯著之影響,未來可藉由選擇不同烹煮方式及條件,生產具有高品質特性之家禽產品。

Chicken meat is well-accepted as a healthier food option compared to red meat. Chicken offers several advantages over red meat that account for an increasing trend in chicken consumption. The unique organoleptic characteristics of native chicken are preferred for the rich flavors and superior texture. Promoting the consumption of Taiwan native chicken (TNC) meat is critical to the industry. Studies on the physicochemical characteristics and descriptive sensory analysis, as well as the relationship between instrumental attributes and human sensory attributes of the TNC and commercial broiler (BR) meat should be conducted. Additionally, the thermal treatment considerably influences the quality and palatability of cooked meat. Therefore, the objectives of this study was to investigate the thermal treatment on physicochemical characteristics, descriptive sensory analysis, and microstructural properties of chicken meat and interpretation of the relationship between meat parameters.
In the experiment 1, it was found that both chicken breeds had significant difference in juiciness, flavor, and meat texture, whereas TNC meat had lower cooking loss after cooking, as a result to higher moisture release and oily mouth coat when analyzed by sensory analysis. The TNC meat had higher textural sensory attributes and provided more intense chicken flavors than BR meat. It clearly demonstrated that the sensory textural attributes vary between BR and TNC meat, and the instrumental texture attributes (e.g., collagen, shear force, cooking loss) were highly correlated with sensory textural attributes (r ≈ 0.7 to 0.9) and influenced cooked meat eating qualities greatly. In the experiment 2, TNC and BR breast meat were heated with various end-point temperatures (50–95°C). With increasing end-point temperature, the cooking loss, collagen solubility, shear force value, and hardness of samples increased in both breeds. BR and TNC meats performed differently upon heating in certain characteristics. The textural attributes had highly correlated with the level of protein solubility and cooking loss of meat. In the experiment 3, the influence of cooking conditions, including laboratory condition “in-bag cooking (BC)”, and real life cooking condition “cooked directly in hot water (WC)”, on the quality of TNC breast meat was evaluated. The results revealed that no significant differences were observed in quality attributes including shear force, collagen solubility, microstructures, redness, yellowness, and descriptive sensory characteristics between treatments. However, BC meat had significantly higher lightness, cooking loss and lower protein solubility after cooking. In experiment 4, TNC breast meat was heated using different cooking methods including, moist-heat (water-cooking; WC) and dry-heat (oven-cooking; OC). The results showed that the OC meat had a significantly higher cooking time, cooking loss, shear force values and darker color than WC meat. Protein solubility decreased after cooking in both cooking methods; however no statistical difference was observed between WC and OC meats, whereas collagen solubility and myofibrillar fragmentation index (MFI) increased after cooking and WC meat exhibited higher collagen solubility and MFI (P < 0.05). The fibril shrinkage was noticeable in OC meat (P < 0.05). Descriptive sensory analysis revealed that WC meat exhibited higher moisture release and lower textural attributes, while OC method enhanced the color and chickeny flavor of TNC meat.
In conclusion, descriptive sensory analysis is capable to identifying and quantifying the important sensory attributes of foods, especially the unique organoleptic characteristics of chicken meat. High relationships of sensory attributes and physicochemical characteristics provide accuracy of information regarding the instrumental and sensory measurements. Moreover, in this study scientifically assessed the effects of different thermal treatments considerably influence the quality and palatability of cooked meat. Therefore, the producers could utilize the information to manufacture poultry products with more desirable qualities.


ACKNOWLEDGEMENT i
摘要 ii
ABSTRACT iv
TABLE OF CONTENTS vi
LIST OF TABLES x
LIST OF FIGURES xi
CHAPTER 1 Literature review and aim of study 1
1.1 Chicken meat consumption 2
1.2 Chicken species 2
1.2.1 Broiler 2
1.2.2 Native chicken 3
1.3 Meat quality 3
1.4 Physicochemical and textural quality 3
1.5 Thermal treatments 4
1.5.1 Moist-heat cooking 4
1.5.2 Dry-heat cooking 5
1.6 Changes in meat quality during heating 6
1.6.1 Physical characteristics 6
1.6.2 Chemical characteristics 8
1.6.3 Texural characteristics 10
1.7 Sensory analysis 11
1.7.1 Consumer affective methods (Hedonic test) 11
1.7.2 Laboratory/analytical methods (Descriptive analysis) 12
Aim of this study 14
CHAPTER 2 Relationships between descriptive sensory attributes and physicochemical analysis of broiler and Taiwan native chicken breast meat 15
2.1 Abstract 16
2.2 Introduction 16
2.3 Materials and methods 19
2.3.1 Sample collection and preparation 19
2.3.2 Proximate composition and pH value 19
2.3.3 Total collagen and soluble collagen contents 19
2.3.4 Cooking loss 20
2.3.5 Color 20
2.3.6 Shear force value 20
2.3.7 Sensory evaluation 21
2.3.8 Statistical analysis 22
2.4 Results and discussion 22
2.4.1 Proximate composition and pH value 22
2.4.2 Cooking loss 22
2.4.3 Sensory analysis and correlation between parameter 23
2.4.3.1 Texture attributes 23
2.4.3.2 Flavor attributes 27
2.4.3.3 Appearance attributes 28
CHAPTER 3 Influences of end-point temperature on the quality attributes of chicken meat................ 35
3.1 Abstract 36
3.2 Introduction 36
3.3 Materials and methods 38
3.3.1 Sample preparation 38
3.3.2 Cooking time and cooking loss 38
3.3.3 Protein solubility and myofibrillar fragmentation index 38
3.3.4 Collagen content and collagen solubility 39
3.3.5 Differential scanning calorimetry 40
3.3.6 Instrumental texture parameters 40
3.3.7 Descriptive sensory analysis 41
3.3.8 Statistical analyses 41
3.4 Results and discussion 41
3.4.1 Cooking time and cooking loss 41
3.4.2 Protein solubility and MFI 42
3.4.3 Collagen content and collagen solubility 44
3.4.4 Thermal analysis 45
3.4.5 Instrumental texture parameters 45
3.4.6 Sensory characteristics 46
3.4.7 Relationship evaluation 47
CHAPTER 4 Validation of feasibility and quality of chicken breast meat cooked under various water cooking conditions 58
4.1 Abstract 59
4.2 Introduction 59
4.3 Materials and methods 61
4.3.1 Sample preparation 61
4.3.2 Physicochemical analysis 61
4.3.2.1 Proximate compositions 61
4.3.2.2 pH value 62
4.3.2.3 Cooking loss and color 62
4.3.2.4 Shear force value 62
4.3.2.5 Protein solubility 62
4.3.2.6 Collagen solubility 63
4.3.3 Microstructural analysis 63
4.3.4 Descriptive sensory evaluation 64
4.3.5 Statistical analysis 65
4.4 Results and discussion 65
4.4.1 Proximate composition, cooking time, and cooking loss 65
4.4.2 Shear force 66
4.4.3 Protein solubility and collagen solubility 67
4.4.4 Microstructural analysis 69
4.4.5 Instrumental color measurement and descriptive sensory evaluation 70
CHAPTER 5 Effects of moist- and dry-heat cooking on the meatquality, microstructure, and sensory characteristics of native chicken meat 77
5.1 Abstract 78
5.2 Introduction 78
5.3 Materials and Methods 80
5.3.1. Sample preparation 80
5.3.2 Physicochemical analysis 80
5.3.2.1 Cooking time, cooking loss, and color 80
5.3.2.2 Shear force value 81
5.3.2.3 Myofibrillar fragmentation index 81
5.3.2.4 Protein solubility and collagen solubility 81
5.3.3 Microstructural analysis 82
5.3.4 Descriptive sensory evaluation 82
5.3.5 Statistical analysis 83
5.4 Results and Discussion 83
5.4.1 Cooking time, cooking loss, color, and shear force 83
5.4.2. MFI value, solubility of protein and collagen 85
5.4.3. Microstructural analysis 86
5.4.4. Descriptive sensory evaluation 87
CHAPTER 6 92
6.1 Conclusion 93
6.2 Future research 94
REFERENCES 95
APPENDICS 108



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