臺灣博碩士論文加值系統

本研究之目的為建立薄葉嘉賜木組織培養之微體繁殖體系。果實消毒以億力( 1g/L )及鏈黴素( 1g/L )溶液中浸泡1小時，流水處理1小時，再以濃度75%的酒精浸泡並手搖30秒，再浸於5%次NaOCl溶液中，在超音波震盪器10分鐘後移於無菌操作檯，以ddH2O清洗3-4次，在無菌操作台中取出種子或胚進行試管培養，結果顯示無污染率約99.6%，發芽率高於6成，並建立無菌苗的繁殖體系。在器官形成方面:以無菌苗頂芽與帶芽莖段較佳之培殖體，其在含有不同植物生長調節劑的WPM培養基中皆達9成以上的芽體誘導率，其中以添加1mg/L BA及同時添加 1mg/L BA和0.1mg/L NAA的培養基誘導率100.00%最高。另外添加0.5mg/L BA的培養基平均誘導芽體數2.69個最高。不同培殖體間以頂芽誘導芽體率100.00%最高，且以帶多個芽點的莖段產出芽體數2.60最高。此外子葉以及下胚軸亦可以誘導出芽體。發根試驗中，以添加0.1mg/L NAA之WPM培養基誘導率100.00%最高，其餘添加NAA之培養基的發根效果亦佳。此外，未添加植物生長調節劑的與添加活性碳之培養基誘導發根之能力也很好。
由誘導芽體產生之完整小苗經馴化6周後存活率達96%。

The main objective of this study is to establish the micropropagation system of Casearia membranace Hance.
The fruits are sterilized with 1g/L benomyl and 1g/L streptomycin for 1 hour, and treated with running tap water for 1 hour. The fruits were then shaken in 70% ethanol solution for 30 sec, and soaked in NaOCl with ultrasonic shaking for 10 min afterwards. Next, we washed them with double distilled water for 3-4 times in laminar air flow chamber. Then we took out the seeds or the embryos by aseptic manipulation. The result showed the contamination rate was less than 1%, and the germination rate is more than 60%. This process can successfully establish aseptic plantlets.
In the aspect of organ formation: the aseptic shoot tips and nodals are the best explants for shoot induction. Those cultured in WPM medium with several different PGRs compositions all showed more than 90% induction rate. WPM+1mg/L BA and WPM+1mg/L BA+0.1mg/L NAA medium were the best among them, which showed 100.00% induction rate. In addition, WPM+0.5mg/L BA medium showed the maximum shoots, which was in average 2.69 shoots. Furthermore, cotyledons and hypocotyl segments can also induct shoots. The result of root induction test showed that the WPM+0.1mg/L NAA medium was the best one. In addition, other NAA added mediums were also accessible. Moreover, blank WPM medium and WPM with activated carbon mediums were suitable for root induction for Casearia membranacea at the same time.
Through micropropagation process as above, these complete plantlets achieved 96% survival rate after 6-week acclimatization.

謝誌 i
摘要 ii
Abstract iii
目錄 iv
圖目錄 vi
表目錄 vii
縮寫表(Abbreviations) viii
一、前言 (Introduction) 1
二、前人研究 (Literature Review) 3
(一) 嘉賜木屬植物介紹 3
(二) 嘉賜木屬植物的藥用成分 4
1. 萜類 4
2. 苯丙烷類、類黃酮與其他酚類 6
3. 固醇類與其他化合物 6
4. 精油 7
(三) 薄葉嘉賜木的藥用價值 7
(四) 植物組織培養 10
1. 培殖體 11
2. 表面消毒 11
3. 培養基 11
4. 植物生長調節劑 11
5. 培養環境 12
6. 植物組織培養方法 12
三、材料與方法(Materials and Methods) 17
(一) 材料 17
1. 無菌苗培養 17
2. 芽體誘導 17
3. 芽體抽長 17
4. 誘導發根 17
5. 馴化 17
(二) 方法 21
1. 培養基 21
2. 培養環境 21
3. 試驗項目 21
四、結果與討論(Results and Discussions) 31
(一) 表面消毒 31
1. 種子處理 31
2. 野外頂芽與側芽之表面消毒 31
(二) 無菌苗培養 32
(三) 芽體誘導試驗 35
1. 野外材料芽體誘導試驗 35
2. 子葉誘導芽體試驗 40
3. 下胚軸誘導芽體試驗 52
4. 頂芽與帶芽莖段芽體誘導試驗 60
(四) 芽體抽長試驗 68
1. 繼代培養 68
2. 抽長試驗 68
3. 暗室培養之芽體抽長試驗 69
(五) 發根誘導試驗 70
1. 添加植物生長調節劑 70
2. 未添加植物生長調節劑 70
(六) 馴化 78
五、結論(Conclusions) 81
引用文獻(Reference) 83

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