臺灣博碩士論文加值系統

表面經過修飾的超順磁奈米粒子，目前已知應用於生物分離、核磁共振造影、熱治療和藥物輸送。而一些生物信號分子，如：膽汁酸、類固醇和膽固醇衍生物等，都屬於自然界中的兩性分子。在醫學臨床治療上，吸附膽汁酸可以降低血液中膽固醇的濃度；染整工業廢水中的色素也屬於兩性分子，在處理染整工業廢水的同時必須移除色素。基於這類的需求，希望製備一種可吸附兩性分子化合物也可使用在生醫上的磁性奈米粒子。因此，本研究製備帶正電荷且具疏水性官能基的超磁性二氧化矽奈米粒子。以螢光素鈉(一種酸性兩性分子染料)作為吸附模型，研究功能性磁性二氧化矽奈米粒子的吸附機制。

Superparamagnetic nanoparticles with functional surface modification have been applied to bioseparation, magnetic resonance imaging, hyperthermia therapy and drug delivery. Several signaling biomolecues, e.g., steroids and cholesterol derivatives, are amphiphilic. It is thus desirable to prepare a magnetic nanoparticle capable of adsorbing amphiphilic compounds, which may be used in a variety of biomedical applications. Accordingly, the superparamagnetic magnetic silica nanoparticles modified with basic and hydrophobic functional groups were prepared in this work. The role of surface nature of the functional magnetic nanoparticles in adsorption of sodium fluorescein (an acidic amphiphilic dye) was examined.

摘要 II
Abstract III
致謝 IV
目錄 V
圖目錄 VIII
表目錄 IX
表目錄 IX
第一章 前言 1
1-1 磁性理論簡介 1
1-2 磁性奈米粒子之性質 7
1-3 氧化鐵之性質 10
1-4 核殼型磁性奈米粒子之製備與應用 14
1-4-1 核殼型磁性奈米粒子之製備 16
1-4-2 核殼型磁性奈米粒子在生醫工程之應用 21
1-4-3 核殼型磁性奈米粒子在生化分離之應用 23
1-5 研究動機與目的 24
第二章 實驗材料與方法 26
2-1 實驗方法與分析儀器設備 26
2-1-1 化學藥品 26
2-1-2 實驗設備 28
2-1-3 分析儀器 30
2-2 實驗步驟 31
2-2-1 利用化學共沉澱法製備磁性氧化鐵奈米粒子 31
2-2-2 以Stöber process製備磁性二氧化矽粒子 31
2-2-3 胺基修飾之磁性二氧化矽粒子之製備 32
2-2-4 四級銨修飾之磁性二氧化矽粒子之製備 32
2-3 儀器分析 38
2-3-1 X光繞射儀 38
2-3-2 穿透式電子顯微鏡 39
2-3-3 傅立葉轉換紅外線光譜儀 40
2-3-4 熱重分析儀 40
2-3-5 超導量子干涉儀 41
2-3-6 固態核磁共振波譜儀 42
2-3-7 全自動接觸角測試儀 42
第三章 結果與討論 45
3-1 磁性二氧化矽奈米粒子之合成與結構分析 45
3-1-1 穿透式電子顯微鏡分析 45
3-1-2 X光粉末繞射分析 47
3-1-3 磁性量測 49
3-2 磁性二氧化矽奈米粒子表面四級銨官能基之鑑定 51
3-2-1 傅立葉轉換紅外線光譜儀分析 51
3-2-2 固態核磁共振波譜儀分析 51
3-2-3 熱重分析 53
3-2-4 接觸角量測 53
3-3 材料吸附性測試 58
第四章 結論 62
參考文獻 63

林千惠(2009)，溶膠凝膠法製備奈米級SiO2顆粒之探討，化工資訊與商情。
張立德、牟季美(2002)，奈米材料和奈米結構，滄海書局。
周禮君、田珮(2000)，有機矽烷氧基前驅物衍生的有機-無機混成溶凝膠材料，化工技術。
莊萬發編撰(1995)，超微粒子理論應用，復漢出版社。
陳文照、曾春風、游信和譯(2008)，材料科學與工程導論，高立圖書有限公司。
陳威倩(2001)，以g -> a-Fe2O3相變機制製作alpha-Fe2O3微粒，國立成功大學資源工程研究所碩士論文。
魏明芬(2002)，磁性金屬氧化物奈米粒子合成與鑑定，中正大學化學研究所碩士論文。
許樹恩、吳泰伯(2006)，X光繞射原理與材料結構分析，中國材料科學學會。
Ayyub, P., Multani, M., Barma, M, Palkar, V. R., Vijayaraghavan, R. (1988). Size-induced structural phase transitions and hyperfine properties of microcrystalline Fe2O3. J. Phys. C: Solid State Phys., 21, 2229-2245.
Belyakova, L. A., Besarab, L. N., Roik, N. V., Lyashenko, D. Y., Vlasova, N. N., Golovkova, L. P., Chuiko, A. A. (2006). Designing of the centers for adsorption of bile acids on a silica surface. J. Colloid Interface Sci., 294, 11-20.
Caruso, F. (2001). Nanoengineering of particle surfaces. Adv. Mater., 13, 11-22.
Cerneaux, S., Zakeeruddin, S. M., Pringle, J. M., Cheng, Y.-B., Grätzel, M., Spiccia, L. (2007). Novel nano-structured silica-based electrolytes containing quaternary ammonium iodide moieties. Adv. Funct. Mater., 17, 3200-3206.
Fan, J., Lu, J., Xu, R., Jiang, R., Gao, Y. (2003). Use of water-dispersible Fe2O3 nanoparticles with narrow size distributions in isolating avidin. J. Colloid Interface Sci., 266, 215-218.
Gupta, A. K., Guptab, M. (2005). Synthesis and surface engineering of iron oxide nanoparticles for biomedical applications. Biomaterials, 26, 3995-4021.
Hervé, K., Douziech-Eyrolles, L., Munnier, E., Cohen-Jonathan, S., Soucé, M., Marchais, H., Limelette, P., Warmont, F., Saboungi, M. L., Dubois, P., Chourpa, I.(2008). The development of stable aqueous suspensions of PEGylated SPIONs for biomedical applications. Nanotechnology, 19, 465608.
http://www.ndt-ed.org/EducationResources/CommunityCollege/MagParticle/Physics/HysteresisLoop.htm
Iller, R. K. (1979). The chemistry of silica. Wiley, New York.
Jiles, D.(1998). Introduction to magnetism and magnetic materials. The Taylor ＆ Francis Group. New York.
Lee, J., Isobe, T., Senna, M. (1996). Preparation of ultrafine Fe3O4 particles by precipitation in the presence of PVA at high pH. J. Colloid Interface Sci. 177, 490-494.
Klabunde, K. J. (2001), Nanoscale materials in chemistry. Wiley, New York.
Ma, Z. Y., Guana, Y. P., Liu, H. Z. (2006). Superparamagnetic silica nanoparticles with immobilized metal affinity ligands for protein adsorption. J. Magn. Magn. Mater., 301, 469-477.
Niemeyer, C. M. (2001). Nanoparticles, protein, and nucleic acid: biotechnology meets materials science. Angew. Chem.-Int. Edit., 40, 4128-4158.
Ohmori, M., Matijević, E. (1993). Preparation and properties of uniform coated inorganic colloidal particles: 8. silica on iron. J. Colloid Interface Sci., 160, 288-292.
Qiu, Z. Q., Du, Y. W., Tang, H., Walker, J. C. (1988). A Mössbauer study of fine iron particles. J. Appl. Phys., 63, 4100-4104.
Šulek, F., Drofenik, M., Habulin, M., Knez, Z. (2010). Surface functionalization of silica-coated magnetic nanoparticles for covalent attachment of cholesterol oxidase. J. Magn. Magn. Mater., 322 ,179-185.
Yang, C. Q., Wang, G., Lu, Z. Y., Sun, J., Zhuang, J. Q., Yang, W. S. (2005). Effect of ultrasonic treatment on dispersibility of Fe3O4 nanoparticles and synthesis of multi-core Fe3O4/SiO2 core/shell nanoparticles. J. Mater. Chem., 15, 4252-4257.
Yang, P. F., Chin, M. C., Lee, C.K. (2007). Interaction of hyaluronic acid (HA) with organosilicon (Si-QAC) modified magnetite for HA recovery. Sep. Sci. Technol., 42, 1747-1760.
Yang, P. P., Quan, Z. W., Hou, Z. Y., Li, C. X., Kang, X. J., Cheng, Z. Y., Lin, J. (2009). A magnetic, luminescent and mesoporous core–shell structured composite material as drug carrier. Biomaterials, 30, 4786-4795.
Zhang, L., Qiao, S. Z., Jin, Y. G., Yang, H. G., Budihartono, S., Stahr, F., Yan, Z. F., Wang, X. L., Hao, Z. P., Lu, G. Q. (2008). Fabrication and size-selective bioseparation of magnetic silica nanospheres with highly ordered periodic mesostructure. Adv. Funct. Mater., 18, 3203-3212.