臺灣博碩士論文加值系統

廚房油煙中含有相當多的化學物質，其中醛類物質是油煙中特徵排放物，長鍊的醛類物與油脂中的脂肪酸氧化相關；除此之外，有些醛類物質為致癌物或疑似致癌物，量測油煙中的醛類物質為一重要的研究課題，然而，目前針對油煙中醛類物質完整的量測方法闕如，因此本研究目的是建立有效而方便的C1~C10醛類物質的氣粒狀採樣分析方法，同時探討採樣介質之回收效率，及實際廚房環境的應用，以評估油煙中醛類物質的濃度分佈，了解暴露油煙的相關資料。
本研究使用DNPH-裹附的玻璃濾紙串聯DNPH吸附管以採集油煙中粒相、與氣相的醛類物質，樣本以高效能液相層析儀/紫外可見光偵測儀分析。實驗室的評估結果顯示此方法的線性範圍0.995 ~ 0.999 (R2)，偵測下限為0.002 ~ 0.014μg/mL，再現性為2.37% ~ 6.78% (CV%)，脫附效率為80% ~ 100%，皆符合環境偵測的規範；實際採樣上，發現此方法適用於廚房油煙的樣分析。

There exist a lot of chemicals in cooking oil fumes (COFs), especially long-chain aldehydes, which are frequently found in COFs and exclusively in oil-related emissions. It is important to measure the emissions of aldehydes, such as trans,trans-2,4-decadienal which is a potential tumor promoter and can be served as a characteristic compound of COFs. Few studies have investigated aldehyde distributions of COFs comprehensively; therefore, this study establishes a C1~C10 aldehyde sampling and analysis method for both particulate- and gas phases, and applies this method to evaluate concentration distribution of aldehydes in COFs.
The cooking oil fumes were collected using DNPH-coated glass fiber filters (25 mm) and 2,4-DNPH cartridges (47285-U SUPERLCO) connected in serial for measuring particulate- phase and gas-phase aldehydes, respectively. After sampling, samples were analyzed by HPLC/UV in gradient mode. This method was optimized for 20 carbonyl chemicals analysis. This method was evaluated in terms of linearity, method detection limit, reproducibility and desorption efficiency in the laboratory. After laboratory evaluation, this method was applied in five kitchens for field assessment. Results show that linearity, method detection limit, reproducibility (expressing as CV) are 0.995-0.999 (R2), 0.002-0.014 μg/mL and 2.37-6.78 % (CV), respectively. The recovery rates of 2,4-DNPH cartridges are 80 ~ 100% for concentration range between 0.2678- 0.3349 μg/ml. They are within in the acceptable range. This method also shows applicable for field test.

目錄
摘要 I
Abstract II
目錄 III
圖目錄 VII
表目錄 VIII
第一章 前言 1
1-1 研究動機 1
1-2 研究目的 3
第二章 文獻回顧 4
2-1 油煙的來源 4
2-2 油煙的成分 4
2-3 油煙中醛類物質 6
2-3-1 Trans,trans-2,4-decadienal 7
2-4 醛類物質之基本性質 9
2-5 醛類物質之危害性 13
2-6 醛類物質之採樣技術 14
第三章 研究設備與方法 19
3-1 整體研究架構 19
3-2 醛類衍生物製備 22
3-3 分析儀器 25
3-4 檢量線製作 26
3-5 迴歸係數，再現性，偵測極限與分析品管規劃 27
3-6 採樣介質 29
3-6-1 粒相採樣介質 29
3-6-2 氣相採樣介質 31
3-7 長碳鍊醛類脫附效率 34
3-8 廚房採樣設計 37
3-9 廚房採樣策略 39
3-9-1 採樣目的 39
3-9-2 採樣物質 39
3-9-3 採樣設備 40
3-9-4 採樣期間與時間 42
3-9-5 採樣個數 42
3-9-6 採樣品管規劃 43
第四章 結果與討論 45
4-1 實驗室評估 45
4-1-1 純度測試 45
4-1-2 最適化分析條件測定 47
4-1-3 檢量線、再現性、線性範圍與方法偵測下限 51
4-1-4 脫附效率 56
4-1-5 分析之品保品管紀錄 59
4-2 實際採樣分析結果 62
4-2-1 空白樣品 62
4-2-2 採樣方法之可行性 65
4-2-3 各廚房的烹調方式與食物 68
4-2-4 廚房採樣結果 68
4-2-4-1 醛類濃度分佈 68
4-2-4-2 廚房氣粒相醛類物質分佈 75
4-2-4-3 廚房採樣位置與醛類濃度關係 83
4-2-4-4 烹調方式與醛類物質之相關 85
第五章 結論與建議 88
5-1 結論 88
5-2 建議 89
第六章 參考文獻 90
附件一 廚房採樣器材清單 98
附件二 幫浦校正紀錄表 99
附件三 採樣分析記錄表 100
附件四 婦女廚房採樣問卷 101

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