臺灣博碩士論文加值系統

本論文以本實驗室於中油冷卻水系統沉積物分離出來之Shewanella sp. NTOU以包埋固定的方式製成生物固定化顆粒，進行特殊厭氧化處理後測試其在有氧的情況下對於三類合成染劑(偶氮、三甲基苯、蔥醌)脫色分解與脫色後環胺類次產物的分解能力，期望能達到單憑經過特殊處理的固定化顆粒，達成於有氧環境下同時進行厭氧脫色，與有氧環胺類產物分解雙重效果之可能性。實驗結果發現進行過特殊厭氧化處理之固定化顆粒在以混合比例為4:10(w/w)，特定之pH(含剛果紅之脫色液調整為6.0；另外含其他三種染劑之脫色液調整為9.0)、溫度30℃、鹽度3%、Yeast extract含量0.125% 在微有氧或絕對有氧情況下對濃度為100 ppm 各染劑的脫色率於32小時內可達到90%以上。在環胺類副產物分解能力的部份其亦可將偶氮染劑Congo red的環胺類次產物Benzidine分解，所需時間為7天；Orange G的環胺類次產物Aniline約28小時後會開始進行分解；對蔥醌染劑Reactive blue-19的次產物1,4 - dihydroxy- 9,10 anthracenedione則約10日後會開始進行分解。此固定化顆粒重複使用性估計可達6次以上，每次對偶氮染劑Congo red 或是三甲基苯染劑Crystal violet與Malachite green有90% 的脫色率，對蔥醌染劑Reactive Blue-19有75% 以上的脫色能力，若在最適的狀況下最大分解效能約為: 剛果紅12.42 mg/l･g･Hr，結晶紫為21.74 mg/l･g･Hr，孔雀綠為86.96 mg/l･g･Hr，RB-19為9.28 mg /l･g･Hr。。在保久能力方面，於下4℃保存兩週後其對Congo red、Crystal violet、Malachite green 仍有90%以上的脫色率，對Reactive Blue-19則有75%以上的脫色能力。本論文實驗之結果，可說明此經過厭氧化處理之固定化顆粒可於微有氧或絕對有氧狀況下，進行染劑厭氧脫色與有氧環胺類次產物之分解外，重複使用效能高，低溫保存性良好。相較於目前應用之傳統的有氧 - 厭氧雙生物反應槽方式應有更多元的染整廢水業處理用途。

Textile wastewaters were toxic and caused serious environmental problems. Previous studies had shown that decoloration of synthetic dyes, especially azo dyes can be achieved by using reduction of these dyes under anaerobic conditions. However, the intermediate,aromatic amines, produced from reduction of these dyes are generally not degraded and accumulated under anaerobic conditions, but could be degraded under aerobic conditions.Based on this metabolism, sequencial batch water system had been established to that waste water efferent containing azo dyes. In this study, we made immobilized beads with Shewanella sp. NTOU1 in an attempt to degrade three kinds of synthetic dyes(Azo, Triphenylmethane and Anthraquinone dyes)inside the beads which were under anaerobic conditions and degrade their intermediates(Aromatic amine) outside the beads which were under aerobic conditions. We expected to make simultaneous anaerobic and aerobic degradation of synthetic dyes in aerobic or microaerophilic environments. In optimum conditions, more than 90% of these dyes(100 ppm)could be removal in 24 hours. Benzidine, the intermediates could be degraded in next 10 days and the other intermediates Aniline, could be degraded after 28 hours. The degradtion product of RB-19, 1,4 - dihydroxy- 9,10 anthracenedione could be degraded after 10 days. These immobilized beads showd very high durability and stability. Compared with the two stage anaerobic-aerobic treatment system and normal wastewater treatment system, these immobilized cells have higher applicability and efficiency in textile wastewaters treatment.

謝辭…………………………………………………………………i
中文摘要……………………………………………………………iii
英文摘要……………………………………………………………v
目錄…………………………………………………………………vii
表目錄………………………………………………………………viii
圖目錄……………………………………………………………… x
壹、前言…………………………………………………………… 1
各類型染劑簡介…………………………………………………… 1
染劑廢水的影響與處理方法……………………………………… 6
台灣染劑廢水處理現況…………………………………………… 11
Shewanella菌屬介紹……………………………………………… 14
生物固定化技術簡介……………………………………………… 23
貳、材料與方法…………………………………………………… 31
叁、結果…………………………………………………………… 49
肆、討論…………………………………………………………… 66
伍、參考文獻……………………………………………………… 81
圖表………………………………………………………………… 88

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