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研究生:黃靜惠
研究生(外文):Ching-Hui, Huang
論文名稱:粒線體DNA的低拷貝數與代謝症候群之相關性
論文名稱(外文):Low Copy Number of Mitochondrial DNA Is Associated with Metabolic Syndrome
指導教授:鄭瑞棠鄭瑞棠引用關係
學位類別:碩士
校院名稱:長榮大學
系所名稱:醫學研究所
學門:醫藥衛生學門
學類:醫學學類
論文種類:學術論文
論文出版年:2009
畢業學年度:97
語文別:中文
論文頁數:62
中文關鍵詞:粒線體DNA粒線體DNA拷貝數代謝症候群
外文關鍵詞:mitochondrial DNAcopy number of mitochondrial DNAmetabolic syndrome
相關次數:
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研究背景與目的:代謝症候群是指血壓及血糖偏高、血脂異常等一群代謝危險因子群聚現象,為國人新興的健康議題。代謝症候群會伴隨增加罹患第二型糖尿病及心臟血管疾病的風險。代謝症候群及其相關疾病可能造成缺陷的原因為細胞產生過多的氧化壓力與粒線體質量的改變。有研究指出粒線體DNA拷貝數量的減少,會大幅增加罹患第二型糖尿病及心臟血管疾病的風險。因此本研究計畫擬探討白血球粒線體DNA拷貝數與代謝症候群進展之相關性。
研究方法:共計有121位台灣人(79位控制組和42位代謝症候群者),依其代謝症候群診斷條件中的五項危險因子之多寡與有無,分析其粒線體的變化。粒線體DNA 基因拷貝數測定以定量聚合酶連鎖反應法和即時聚合酶鏈鎖反應(Real-time PCR)測之。統計分析採用student t test, ANOVA多變項分析及Spearman’s rho correlation analysis.
結果: 代謝症候群組與控制組相比,白血球粒線體DNA拷貝數顯著低下。粒線體DNA拷貝數與HDL成正相關,而與體重、收縮壓、舒張壓、尿酸和RIFACTOR (Cholerterol/HDL) 成負相關。代謝症候群組血漿中有較高的insulin(胰島素)、creatinine、urate、oxLDL、ApoB 、RIFACTOR 及homocystein;且有較低的 HDL、ApoA 與adiponectin.
結論:粒線體DNA的低拷貝數,在代謝症候群中扮演重要角色。且提供代謝症候群在診斷及治療策略的參考。
Objective: The metabolic syndrome (MetS) is a group of risk factors of metabolic origin that are accompanied by increased risk for type 2 diabetes mellitus and cardiovascular disease. One of the defects in MetS and its associated diseases is excess cellular oxidative stress and mitochondrial alternation. A low copy number of mitochondrial DNA (mtDNA) has been found to be associated with enhanced risk for the development of diabetes and cardiovascular disease. Therefore, the objective of this study was to clarify the characteristics of mtDNA copy number of leukocytes in MetS.

Methods: Seventy nine control subjects and 42 metabolic syndrome subjects of Taiwanese were recruited and Quantitative polymerase chain reaction (QPCR) and real time PCR were applied in present study. A student t test, multi-variate ANOVA, and Spearman''s rho correlation analysis were used for statistic analysis.

Results: The results revealed that compared to control subjects, a significant lower copy number of mtDNA in the leukocytes of MetS subjects and mtDNA copy number was associated with a significant increase in HDL and inversely correlated with weight, SBP, DBP, Urate and RIFACTOR (cholesterol/HDL). MetS group had higher insulin, creatinine, urate, oxLDL, ApoB, RIFACTOR, and homocystein and lower HDL, ApoA and adiponectin in the plasma.

Conclusion: The present study shows that a low mtDNA copy number of leukocytes play an important role in the progression of MetS. The results may provide new diagnostic and therapeutic strategies to MetS and other associated disorder.
英文縮寫 Ⅲ
中文摘要 Ⅵ
英文摘要 Ⅷ
壹、導論 1
一、代謝症候群 1
1. 定義 1
2. 現況分析 2
3. 代謝症候群致病機轉 3
二、粒線體 4
1. 粒線體的功能與構造 4
2. 粒線體DNA 6
3. 粒線體DNA拷貝數 6
貳、研究構想 10
參、研究材料與方法 11
一、被研究者 11
二、定義代謝症候群 11
三、血壓及抽血 12
四、分離白血球DNA 12
五、粒線體DNA 基因拷貝數測定 12
六、血漿中同半胱氨酸分析 13
七、血漿中ox-LDL分析統計方法 14
八、血漿中ox-LDL antibody分析 14
九、血漿中adiponectin分析 14
十、統計方法 14
肆、結果 15
ㄧ、粒線體DNA基因拷貝數與代謝症候群危險因子個數之關係 15
二、各個影響白血球粒線體DNA拷貝數之因素的線性相關探討 15
三、代謝症候群組與非代謝症候群組兩組臨床資料和生化數值之比較 16
四、血壓高低及高密度膽固醇數值高低對白血球粒線體DNA拷貝數影響之探討 17
伍、討論 18
一、粒線體DNA基因拷貝數與氧化壓力之探討 18
二、粒線體DNA基因拷貝數與代謝症候群危險因子個數之關係 20
三、各個影響白血球粒線體DNA拷貝數之因素的線性相關探討 21
四、代謝症候群組與非代謝症候群組兩組臨床資料和生化數值之比較 29
五、研究限制 36
陸、結論 37
柒、參考文獻 38
捌、表 54

玖、圖 58

拾、附圖 62
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