臺灣博碩士論文加值系統

木瓜(Carica papaya L.)的植株基本上可被區分為兩性株, 雄株及雌株三種.而從木瓜各性別間的雜交及自交的實驗結果顯示出其性別乃是受控於遺傳因子.而目前對於木瓜性別決定基因方面的研究還沒有一個具体的研究報告.本實驗嘗試以逢機擴增多形性DNA來尋找木瓜雄株和木瓜雌株兩者在基因組上的不同之處,以期能從這些不同的基因片段中找出木瓜性別的決定基因.在本實驗中我以購自Operon所生產的逢機引子(primer)來放木瓜基因組中的基因片段．而在測試了兩百種不同的引子後，我篩到由兩段不同引子(M2及O2)所放大的基因片段在雄株與雌株中有不同之處.在以O2片段為探針,來進行南方轉印及DNA雜合反應.其結果顯示在雄性或是雌性的基因組中含有O2 DNA 片段的基因可能沒有不同之處.為了要進一步的選殖木瓜的性別決定基因,更多的逢機引子來進行RAPD的實驗是必須的.

Carica papaya L. is a trimonoecious (polygamous) species that individualplants can be classified to three basic sex types:hermaphrodite, pistillate(female) and staminate (male).The results of cross- and self-pollinations in papaya have showed that the determination of sex type was caused by inherited factors.In this work, we try to clone the different DNA fragments between staminate and pistillate by randomly amplified polymorphic DNA (RAPD).In order to isolate the sex determination genes of papaya, we have screenedwith 200 primers and have cloned two specific DNA fragmentsamplified by respective two primers.A genomic DNA blot analysis by using these two fragments as probes indicates no restriction fragments length polymorphism(RFLP) between pistillate and staminate genomes.In order to isolate thesex determined gene of papaya, it is necessary to use a large number of10-meroligo primer to perform RAPD experiments.